| Effect of voltage sensitive fluorescent proteins on neuronal excitability (Akemann et al. 2009) |
| Accession: 123453 |
"Fluorescent protein voltage sensors are recombinant proteins that are designed as genetically encoded cellular
probes of membrane potential using mechanisms of voltage-dependent modulation of fluorescence.
Several such proteins,
including VSFP2.3 and VSFP3.1, were recently reported with reliable function in mammalian cells.
...
Expression of these proteins in cell membranes is accompanied by additional dynamic membrane capacitance, ...
We used recordings of
sensing currents and fluorescence responses of VSFP2.3 and of VSFP3.1 to derive kinetic models of the voltage-dependent
signaling of these proteins.
Using computational neuron simulations, we quantitatively investigated the perturbing effects of
sensing capacitance on the input/output relationship in two central neuron models, a cerebellar Purkinje and a layer 5 pyramidal
neuron.
... ". The Purkinje cell model is included in ModelDB.
Reference: Akemann W, Lundby A, Mutoh H, Knopfel T (2009) Effect of voltage sensitive fluorescent proteins on neuronal excitability. Biophys J 96:3959-76 [PubMed] |
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This is the readme.txt for the model associated with the paper:
Walther Akemann, Alicia Lundby, Hiroki Mutoh, Thomas Knopfel. Effect
of voltage sensitive fluorescent proteins on neuronal
excitability. Biophys. J. 2009; 96: 3959-3976
These model files were contributed by Walther Akemann and Thomas
Knopfel.
The files contain the descriptor files for active conductances in a
monocompartment Purkinje cell model. Ion channel gating currents
(igate) are calculated from the first derivative of the state
dynamics.
Two voltage-sensitive fluorescent proteins, VSFP2.3 and VSFP3.1, are
modeled with a 8-state reaction (refered to as Model III in the paper)
to represent the sensing and fluorescence dynamics of these proteins.
To reproduce the simulations shown in Fig. 9 (left panel). Simply
auto-launch from ModelDB (after NEURON is installed) or download and
extract the archive (a new folder is created), then compile the mod
files (and start) under
Linux:
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by executing "nrnivmodl" in the expanded folder. Then type
"nrngui mosinit.hoc".
mswin:
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by running mknrndll (select the new folder). Then in Windows Explorer
double click on the mosinit.hoc file
MAC OS X:
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by dragging and dropping the new folder onto the mknrndll icon. Drag
and drop the mosinit.hoc file onto the nrngui icon.
Once the simulation has loaded:
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Press "Start". The simulation takes a little under 30 seconds on a
2.8 GhZ Pentium 4. The simulations from the left panel of figure 9
appears:
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