[Data]
Odor Map
Namec-fos mRNA expression patterns for male mouse urine odors
Description
SpeciesMouse
Strain
SexFemale
Age12 to 20 weeks
Weight
Brain RegionOlfactory bulb
Sub RegionGlomerular layer
Side
ViewVentral
Serial Number
Odor
Odorant Blend
Male Mouse Urine (H-2b) show other
Male Mouse Urine (H-2 bm1) show other
Male Mouse Urine (H-2 bm8) show other
Concentration20% v/v (1:5 dilution)
Exposure Time30 minutes
Exposure Frequency3 min odor on followed by 2 min odor off
Method
c-fos in situ hybridization show other
NotePrincipal Component Analysis (PCA) of c-fos mRNA expression patterns for H-2b, H-2k, H-2bm8, and H-2bm1 urine odors. A, The location of the individual components of the resulting PCA shown in two dimensions. B, A plot demonstrating that the first two PCs can completely separate the congenic haplotypes H-2k and H-2b. C, Shown is the space defined by the first three components of the PCA. Urine odors are organized into different families according to the distribution of c-fos mRNA glomerular activation patterns within this three-dimensional space. Each sphere represents a single mouse exposed to a single sample of urine. Spheres of the same color represent a single haplotype. D-F, J-X, c-fos glomerular activation patterns for H-2b, H-2bm8, and H-2bm1 male urine odor in the main olfactory bulbs of H-2d female mice. D-F, Color contour maps of averaged evoked glomerular activity. D, H-2bm8 odor map (n = 7); E, H-2bm1 odor map (n = 8); F, H-2b odor map (n = 8); G-I, normalized SD (SD/average) maps for H-2bm8 (D), H-2bm1 (E), and (F) H-2b. J-X, Color contour maps of individual evoked glomerular activity. J, M, P, S, V, H-2bm8 odor maps; K, N, Q, T, W, H-2bm1 odor maps; L, O, R, U, X, H-2b odor maps. Color bar on top of D and J shows the density of active glomeruli (number of positive glomeruli per bin). Color bar on top of G shows the range of the variability (2 is most variable).
AuthorsSchaefer ML, Yamazaki K, Osada K, Restrepo D, Beauchamp GK.
PubMed_ID12417675
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Behavioral Study

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Revisions:2
Last time:5/15/2006 5:08:51 PM
Reviewer:Nian Liu
Owner:Nian Liu
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