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Data
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Correcting space clamp in dendrites (Schaefer et al. 2003 and 2007)
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In voltage-clamp experiments, incomplete space clamp distorts the recorded currents, rendering accurate analysis impossible. Here, we present
a simple numerical algorithm that corrects such distortions. The method enabled accurate
retrieval of the local densities, kinetics, and density gradients of somatic and dendritic channels. The correction method was applied to two-electrode voltage-clamp recordings of K currents from the apical dendrite of layer 5 neocortical pyramidal
neurons. The generality and robustness of the algorithm make it a useful tool for voltage-clamp analysis of voltage-gated
currents in structures of any morphology that is amenable to the voltage-clamp technique.
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Neocortex M1 L5B pyramidal pyramidal tract GLU cell Show
Other
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Neuron or other electrically excitable cell Show
Other
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Schaefer AT, Helmstaedter M, Sakmann B, Korngreen A (2003) Show
Other
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Schaefer AT, Helmstaedter M, Schmitt AC, Bar-Yehuda D, Almog M, Ben-Porat H, Sakmann B, Korngreen A (2007) Show
Other
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Schaefer, Andreas T [andreas.schaefer at crick.ac.uk] Show
Other
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209
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