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Hippocampus CA3 pyramidal cell
GM Shepherd, Synaptic Organization of the Brain, New York: Oxford University Press 1979.
45
84,87
The principal neuron of region CA3 of the hippocampus. It forms a single cell body layer. It gives rise to an apical dendritic tree and several basal dendrites, which are covered with spines. The apical shaft has thorny excresences which receive the excitatory glutamatergic synapses of the mossy fibers from the dentate granule cells. The output of the cell is carried in an axon which recurs to pass through the apical dendritic tree before becoming the Shafer collateral which has excitatory glutamatergic synapses on the apical dendrites of CA1 pyramidal neurons. The axon also makes excitatory glutamatergic synapses on the dendrites of the CA3 pyramidal neurons as it passes through them.
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 Neuronal Receptors (22)
  
SN Property present CF-Compartment Receptor Connect Note Publication facts
1 Yes Middle basal dendrite Glutamate CA3 Pyramidal Neuron terminals (T)
2 Yes Axon terminal NMDA Unlike postsynaptic NMDAR's, they are potentiatedby physiologically relevant concentrations of taurine >551<
3 Yes Distal basal dendrite Glutamate CA3 Pyramidal Neuron terminals (T)
4 Yes Soma Nicotinic
5 Yes Proximal basal dendrite Glutamate CA3 Pyramidal Neuron terminals (T)
6 Yes Middle apical dendrite Glutamate CA3 Pyramidal Neuron terminals (T)
7 Yes Proximal apical dendrite Glutamate Dentate Granule Cell mossy terminals (T)
8 Yes Distal apical dendrite AMPA Perforant pathway entorhinal pyramidal neuron terminals (T) Recordings from membrane patches of dendrites and soma reveal fast and slow responses to fast application of glutamate, mediated by AMPA amd NMDA receptors, respectively >434<
9 Yes Distal apical dendrite NMDA Perforant pathway entorhinal pyramidal neuron terminals (T) Differential induction of potentiation and depression at commissural and mossy fiber synapses has also been shown by #R#158#E#. Recordings from membrane patches of dendrites and soma reveal fast and slow responses to fast application of glutamate, mediated by AMPA amd NMDA receptors, respectively >434<
10 Yes Middle apical dendrite AMPA CA3 Pyramidal neuron terminals (T) It has also been found that CNQX does not block the intracellular calcium concentration increase normally associated with stratum lucidum stimulation >173<
11 Yes Middle apical dendrite NMDA CA3 Pyramidal neuron terminals (T) Differential induction of potentiation and depression at commissural and mossy fiber synapses has also been shown by >152<
12 Yes Middle apical dendrite mGluR Receptor Notes Dentate Granule Cell mossy terminals (T) It has also been shown (using whole and perforated patch recording from acutely isolated CA3 pyramidal neurons) that application of Glu and quisqualatic acid (in the presence of D-AP5, an NMDAR-antagonist, and CNQX, an AMPAR antagonist) results in responses that consist of an inward current that may be preceded by an outward current. Both of these currents are affected by bath [K] and they had different pharmacological properties (Harata et al, 1996).
13 Yes Middle apical dendrite Gaba CA3 Basket Cell terminals (T) A study using simultaneous intracellular recording from interneurons and pyramidal neurons combined with biocytin cell fills and morphological reconstructions revealed that the interneurons made connections onto the soma and proximal dendrites of the pyramidal neuron and that stimulation of the interneurons evoked IPSPs in the pyramidal neurons. EM microscopy revealed differential numbers of terminals depending on the subcellular locus of the connections. >167<
14 Yes Middle apical dendrite GabaA CA3 Basket Cell terminals (T) There is also evidence that Zn+ can modulate bicuculline-sensitive responses to GABA >165< early in development in rat (studied <8 days old)
15 Yes Middle apical dendrite GabaB CA3 Basket Cell terminals (T) Hilar stimulation has been used to elicit (pharmacologically isolated) IPSPs in CA3 pyramidal neurons (recorded by means of intracellular or whole cell methods depending on the age of the animal). Paired pulse stimulation in this preparation resulted in paired pulse depression, which could be reduced by bath application of CGP35348 (a GABA(B)R antagonist) in adult rats. Neonatal rats (5-7 days old) showed paired pulse depression only within a much shorter range of interstimulus intervals and it was not affected by CGP35348 unless transmitter release was facilitated by raising the bath [Ca2+] and lowering the bath [Mg+] >150<
16 Yes Soma AMPA ----- [ed. note: we are not aware of glutamatergic synapses onto the soma] Recordings from membrane patches of dendrites and soma reveal fast and slow responses to fast application of glutamate, mediated by AMPA amd NMDA receptors, respectively >434<
17 Yes Soma NMDA ----- [ed. note: These data disagree on the presence of NMDA receptors in the soma. For a full description of the properties of NMDA receptors in CA3 pyramidal neurons, please see the apical dendritic compartments.] Recordings from membrane patches of dendrites and soma reveal fast and slow responses to fast application of glutamate, mediated by AMPA amd NMDA receptors, respectively >434<
18 Yes Soma mGluR ----- It has also been shown (using whole cell and perforated patch recording from acutely isolated CA3 pyramidal neurons) that application of Glu and quisqualatic acid (in the presence of D-AP5, an NMDAR-antagonist, and CNQX, an AMPAR antagonist) results in responses that consist of an inward current that may be preceded by an outward current. Both of these currents are affected by bath [K] and they had different pharmacological properties (Harata et al. 1996 #8680866).
19 Yes Soma Gaba CA3 Basket Cell terminals (T) A study using simultaneous intracellular recording from interneurons and pyramidal neurons combined with biocytin cell fills and morphological reconstructions revealed that the interneurons made connections onto the soma and proximal dendrites of the pyramidal neuron and that stimulation of the interneurons evoked IPSPs in the pyramidal neurons. EM microscopy revealed differential numbers of terminals depending on the subcellular locus of the connections. >167<
20 Yes Soma GabaA CA3 Basket Cell terminals (T) There is also evidence that Zn+ can modulate bicuculline-sensitive responses to GABA >165< early in development in rat (studied <8 days old)
21 Yes Soma GabaB CA3 Basket Cell terminals (T) Hilar stimulation has been used to elicit (pharmacologically isolated) IPSPs in CA3 pyramidal neurons (recorded by means of intracellular or whole cell methods depending on the age of the animal). Paired pulse stimulation in this preparation resulted in paired pulse depression, which could be reduced by bath application of CGP35348 (a GABA(B)R antagonist) in adult rats. Neonatal rats (5-7 days old) showed paired pulse depression only within a much shorter range of interstimulus intervals and it was not affected by CGP35348 unless transmitter release was facilitated by raising the bath [Ca2+] and lowering the bath [Mg+] >150<
22 Yes Axon terminal NO Experimental findings support a cascade for induction of homosynaptic, NO-dependent LTD involving activation of guanylyl cyclase, production of guanosine 3',5' cyclic monophosphate and subsequent PKG activation. This process has an additional requirement for release of Ca2+ from ryanodine-sensitive stores >263<
 Neuronal Currents (22)
  
SN Property present CF-Compartment Current Connect Note Publication facts
1 Yes Distal apical dendrite I p,q
2 Yes Middle apical dendrite I p,q
3 Yes Proximal apical dendrite I p,q
4 Yes Axon hillock I Na,t
5 Yes Axon hillock I K
6 Yes Axon fiber I Na,t
7 Yes Axon terminal I N
8 Proximal apical dendrite I Na,t Extracellular recordings in vivo suggested that the dendritic density of these channels rapidly decreases with distance from soma >399<
9 Yes Soma I K,Ca In situ hybridization of three cloned SK channel subunits (SK1-3), the prime candidates likely to underlie Ca(2+)-dependent AHPs showed high levels of expression in regions presenting prominent AHP currents including CA1-3 regions of the hippocampus (SK1 and SK2), reticularis thalami (SK1 and SK2), supraoptic nucleus (SK3), and inferior olivary nucleus (SK2 and SK3) >194<
10 Yes Proximal apical dendrite I K A combined in situ hybridization and immunocytochemical study demonstrated that Kv1.2 (which probably corresponds to I(K) channels) is concentrated in the dendrites of CA3 neurons >173<
11 Yes Soma I h Depolarizing "sag" during larger hyperpolarizing voltage transients is indicative of Ih current in determinating the passive membrane properties of CA1 pyramidal neurons >432<
12 Yes Distal apical dendrite I K A combined in situ hybridization and immunocytochemical study demonstrated that Kv1.2 (which may correspond to I(K) channels) is concentrated in the dendrites of CA3 neurons >173<
13 Yes Middle apical dendrite I K A combined in situ hybridization and immunocytochemical study demonstrated that Kv1.2 (which probably corresponds to I(K) channels) is concentrated in the dendrites of CA3 neurons >173<
14 Yes Middle apical dendrite I L high threshold and it has been shown that nimodipine (an L-channel antagonist) prevents certain mossy fiber LTP-types from taking place >161<
15 Yes Middle apical dendrite I T low threshold Whole cell recording from acutely isolated rat CA3 pyramidal neurons revealed a transient (59 msec decay time constant) that was inhibited by Ni2+ and amiloride >149<
16 Yes Soma I Na,t Recordings using the intracellular perfusion method showed no differences between the I-V characteristics of CA1 and CA3 neurones for this current. In contrast to this, the steady-state inactivation of both types of neurones was significantly different >292<
17 Yes Soma I L high threshold It has been suggested that voltage-gated calcium channels play a role in LTP (Johnston et al. 1992), and it has been shown that nimodipine (an L-channel antagonist) prevents certain mossy fiber LTP-types from taking place >161<
18 Yes Soma I N Bath application of omega-conotoxin MVIIC blocked (with rapid kinetics) approximately 20% of the high-voltage activated Ca2+ current, suggesting the presence of N-channels measured in whole-cell recordings >166<
19 Yes Soma I T low threshold Whole cell recording from acutely isolated rat CA3 pyramidal neurons revealed a transient (59 msec decay time constant) that was inhibited by Ni2+ and amiloride >149<
20 Yes Soma I p,q Bath application of omega-conotoxin MVIIC (an antagonist of N and P channels) blocked (with slow kinetics) approximately 30% of the high-voltage activated Ca2+ current measured in whole-cell recordings >166<
21 Yes Soma I A In a study of acutely isolated rat cells under whole cell recording across developmental states (Day 6 - Day 29), I(A) was separated from I(K) by subtraction methods. It was found that I(A) was rapidly activated and inactivated and was 80% blocked by 4-AP (4-aminopyridine), but was insensitive to TEA (tetraethylammonium) and dendrotoxin (Klee et al, 1995). The current has also been shown to be modulated by K concentration (Eder et al, 1996), though this effect appears to be restricted to very early in development (Klee et al, 1997).
22 Yes Soma I K A combined in situ hybridization and immunocytochemical study demonstrated that Kv1.2 (which may correspond to I(K) channels) is concentrated in the dendrites of CA3 neurons >173<
 Neuronal Transmitters (1)
  
SN Property present CF-Compartment transmitter Connect Note Publication facts
1 Yes Axon terminal Glutamate CA1 Pyramidal Neuron: Dam
Other categories referring to Hippocampus CA3 pyramidal cell
Computational model.Model Neurons   (44)
Pathological mechanism.Neuron   (1)
2 Objects Relationship (edge).Object Two (target)   (1)
ABA Genes.Neuron   (1)
Neuronal Structure.Neurons   (1)
Revisions: 13
Last Time: 12/18/2015 11:12:17 AM
Reviewer: System Administrator
Owner: System Administrator