Hippocampal Mossy Fiber bouton: presynaptic KV7 channel function (Martinello et al 2019)

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Accession:245417

Reference:
1 . Martinello K, Giacalone E, Migliore M, Brown DA, Shah MM (2019) The subthreshold-active KV7 current limits spike-induced Ca2+ influx in hippocampal mossy fiber synaptic terminals to regulate neurotransmission Communications Biology, in press
Citations  Citation Browser
Model Information (Click on a link to find other models with that property)
Model Type: Synapse;
Brain Region(s)/Organism: Hippocampus;
Cell Type(s):
Channel(s): I A; I CAN; I K,leak; I M; I Na,t; I K;
Gap Junctions:
Receptor(s): AMPA;
Gene(s):
Transmitter(s): Glutamate;
Simulation Environment: NEURON;
Model Concept(s): Action Potentials;
Implementer(s): Migliore, Michele [Michele.Migliore at Yale.edu]; Giacalone, Elisabetta [elisabetta.giacalone at pa.ibf.cnr.it];
Search NeuronDB for information about:  AMPA; I Na,t; I A; I K; I K,leak; I M; I CAN; Glutamate;
/
modeldb_Kv7_MFB
README.html
cacumm.mod *
can2t.mod
kaprox.mod *
kdrca1.mod *
kir.mod
kmb.mod *
naxn_J.mod
fig4_modeldb.hoc
fig4_modeldb.ses
mosinit.hoc
screenshot1.png
screenshot2.png
                            
load_file("nrngui.hoc")
cvode_active(1)
celsius=34
create soma
diam=2
L=3.5

Vrest=-80
tstop=2000

objref stim[20], a
forall{	insert pas cm=1  e_pas=-65 Ra=150 g_pas=1/30000
		
	insert naxj  ena = 50 gbar_naxj=0.12
        insert kdr  ek = -80 gkdrbar_kdr=0.001
	insert kap gkabar_kap=0.01
	insert kmb  sh_kmb=-30 gbar_kmb=0.005
	insert can gcanbar_can = 0.6
	insert cacum tau_cacum=500
	insert kir gkbar_kir=0.021
	
}

proc init() {
	t=0
        forall {
        v=Vrest
        if (ismembrane("naxj") || ismembrane("na3")) {ena=55}
        if (ismembrane("kdr") || ismembrane("kap") || ismembrane("kad")) {ek=-80}
        if (ismembrane("hd") ) {ehd_hd=-30}
	}
	finitialize(Vrest)
        fcurrent()

	cvode.re_init()
	cvode.event(tstop)
}


for i=0, 19 {
	    stim[i] = new IClamp(0.5)
	    stim[i].del=100+20*i
	    stim[i].dur = 0.35
	    stim[i].amp=0.0
}

proc Single_AP(){
	for i=1,19 {stim[i].amp=0.0}
	stim[0].amp=0.03
	gbar_kmb=0.005
	Graph[0].erase_all()
	Graph[0].size(98,106,-80,20)
	Graph[1].size(0,2000,0,0.13)
	Graph[0].exec_menu("Keep Lines")
	Graph[1].exec_menu("Keep Lines")
	Graph[0].erase_all()
	Graph[0].addvar("v(.5)",1,1)
	Graph[1].addvar("soma.cai( 0.5 )",1,1)
	run()
	gbar_kmb=0.00
	Graph[0].exec_menu("Keep Lines")
	Graph[1].exec_menu("Keep Lines")
	Graph[0].addvar("v(.5)",2,1)
	Graph[1].addvar("soma.cai( 0.5 )",2,1)
	run()
	gcanbar_can=0.0
	Graph[0].exec_menu("Keep Lines")
	Graph[1].exec_menu("Keep Lines")
	Graph[0].addvar("v(.5)",3,1)
	Graph[1].addvar("soma.cai( 0.5 )",3,1)
	run()
	gbar_kmb=0.005
	gcanbar_can=0.6
}

proc Train(){
	for i=1,19 {stim[i].amp=0.03}
	gbar_kmb=0.005
	Graph[0].erase_all()
	Graph[0].size(0,600,-80,20)
	Graph[0].exec_menu("Keep Lines")
	Graph[1].erase_all()
	Graph[0].addvar("v(.5)",1,1)
	run()
	gbar_kmb=0.00
	Graph[0].exec_menu("Keep Lines")
	Graph[0].addvar("v(.5)",2,1)
	Graph[1].erase_all()
	run()
	gcanbar_can=0.0
	Graph[0].exec_menu("Keep Lines")
	Graph[0].addvar("v(.5)",3,1)
	Graph[1].erase_all()
	run()
	gbar_kmb=0.005
	gcanbar_can=0.6
}
a = new VBox()
a.intercept(1)
xpanel("Protocols",0)
xbutton("Single AP","Single_AP()")
xbutton("50 Hz train","Train()")
xpanel()
a.intercept(0)
a.map()

load_file("fig4_modeldb.ses")