Human L5 Cortical Circuit (Guet-McCreight)

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We used L5 Pyr neuron models fit to electrophysiology data from younger and older individuals to simulate detailed human layer 5 microcircuits. These circuits also included detailed parvalbumin+ (PV), somatostatin+ (SST), and vasoactivate intestinal polypeptide+ (VIP) inhibitory interneuron models.
Model Information (Click on a link to find other models with that property)
Model Type: Realistic Network; Neuron or other electrically excitable cell;
Brain Region(s)/Organism: Human; Neocortex;
Cell Type(s): Neocortex U1 interneuron basket PV GABA cell; Neocortex U1 interneuron bipolar VIP/CR GABA cell; Neocortex layer 5 pyramidal cell;
Channel(s): I h; I Na,t; I Na,p; I M; IK Skca; I K;
Gap Junctions:
Receptor(s): AMPA; NMDA; Gaba;
Transmitter(s): Glutamate; Gaba;
Simulation Environment: NEURON; LFPy;
Model Concept(s): Aging/Alzheimer`s;
Search NeuronDB for information about:  Neocortex U1 interneuron basket PV GABA cell; Neocortex U1 interneuron bipolar VIP/CR GABA cell; AMPA; NMDA; Gaba; I Na,p; I Na,t; I K; I M; I h; IK Skca; Gaba; Glutamate;
# Human L5 Cortical Circuit Model --- Guet-McCreight-et-al.-2022
Author: Alexandre Guet-McCreight

This is the readme for the model associated with the paper:

Guet-McCreight A, Chameh HM, Mahallati S, Wishart M, Tripathy SJ, Valiante TA, Hay E (2022) Age-dependent increased sag amplitude in human pyramidal neurons dampens baseline cortical activity. Cerebral Cortex.

Network Simulations:
Simulation code associated with the default L5 circuit used throughout the manuscript is in the /L5Circuit/default_circuit/ directory. Additional tests using heterogeneous circuits can be found in the /L5Circuit/heterogeneous/ directory.

To run simulations, install all of the necessary python modules (see lfpy_env.yml), compile the mod files within the mod folder, and submit the simulations in parallel (e.g., see Running younger and older circuits is controlled for by changing the agegroup variable in to either 'y' or 'o', e.g.:

agegroup = 'y' or 'o'

In, the 2nd to last number in the mpiexec command (see below) controls the random seed used for the circuit variance (i.e., connection matrix, synapse placement, etc.), while the last number controls the random seed number used for stimulus variance (i.e. Ornstein Uhlenbeck noise and stimulus presynaptic spike train timing).

mpiexec -n 400 python 1234 1

All code used for analyzing the circuit simulation results is found in the /L5Circuit Analyses/ directory. These codes perform analyses of multiple simulations using several circuit random seeds (e.g. fig 3) or stimulus random seeds (e.g. fig 5).

Single Cell Optimizations and Simulations:
All code associated with single cell optimizations is found in the /Single Cell Modelling/Optimizations directory. For usage of this code, we recommend starting from the file and associated code in either the /L5Pyr_young_AltMorph_Scinet/ or /L5Pyr_old_AltMorph_Scinet/ directories.

Analysis code of single cell optimization results can be found in the /Single Cell Modelling/Population Analysis/ folder. Current-step single-cell simulation code can be found in the /Single Cell Modelling/Current-Step Simulations/ folder. Code for simulating PSP summation of different connection types can be found in the /Single Cell Modelling/PSP Simulations/ folder.