Factors contribution to GDP-induced [Cl-]i transients (Lombardi et al 2019)

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Accession:253369
This models are used to evaluate which factors influence the GDP (giant depolarizing potential) induced [Cl-]I transients based on a initial model of P. Jedlicka
Reference:
1 . Lombardi A, Jedlicka P, Luhmann HJ, Kilb W (2019) Interactions Between Membrane Resistance, GABA-A Receptor Properties, Bicarbonate Dynamics and Cl-Transport Shape Activity-Dependent Changes of Intracellular Cl- Concentration Int J of Mol Sci [PubMed]
Citations  Citation Browser
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell; Dendrite; Synapse;
Brain Region(s)/Organism: Mouse; Hippocampus;
Cell Type(s): Hippocampus CA3 pyramidal GLU cell;
Channel(s):
Gap Junctions:
Receptor(s): GabaA;
Gene(s):
Transmitter(s): Gaba;
Simulation Environment: NEURON;
Model Concept(s): Synaptic Plasticity;
Implementer(s):
Search NeuronDB for information about:  Hippocampus CA3 pyramidal GLU cell; GabaA; Gaba;
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LombardiEtAl2019
Isolated_Dendrite_tauNKCC1__Fig9
cldif_CA3_NKCC1_HCO3.mod *
gabaA_Cl_HCO3.mod *
VDpas.mod *
vecevent.mod *
cell_isolated_dendrite.hoc *
cell_soma_dendrite.hoc *
cell_soma_dendrite_1000um.hoc
cell_soma_dendrite_fine.hoc
Freq_PSC.ses
gabaA_Cl_HCO3.o
GABA-Stim_1xPSC_isolated_dendrite_GUI.hoc
GABA-Stim_2xPSC_Spatial_Sum_isolated_dendrite.hoc
GABA-Stim_2xPSC_Spatial_Sum_isolated_dendrite_enlarged_tauNKCC1.hoc
GABA-Stim_3xPSC_isolated_dendrite_GUI.hoc
GABA-Stim_5xPSC_Freq_isolated_dendrite.hoc
GABA-Stim_5xPSC_Freq_isolated_dendrite_back.hoc
GABA-Stim_5xPSC_Freq_isolated_dendrite_enlarged_tauNKCC1.hoc
GABA-Stim_5xPSC_Spatial_isolated_dendrite-Draft.hoc
init_Cldif_isolated_dendrite.hoc
Isolated_Dendrite.ses *
Isolated_Dendrite_Diff_Flux_1xGABA.ses
Isolated_Dendrite_Diff_Flux_3xGABA.ses
Isolated_Dendrite_Diff_Flux_Laptop.ses
start_GABA-Stim_2xPSC_Spatial_Sum_isolated_dendrite.hoc
start_GABA-Stim_2xPSC_Spatial_Sum_isolated_dendrite_enlarged_tauNKCC1.hoc
start_GABA-Stim_5xPSC_Freq_isolated_dendrite.hoc
start_GABA-Stim_5xPSC_Freq_isolated_dendrite_enlarged_tauNKCC1.hoc
start_procedure.hoc
                            
// Simulation and analysis of Spatiotemporal Component of Cl- Gradients in a isolated dendrite

// Define spatial properties of dendrite
  ANZAHL_NODES = 100

// Determination Parameters GABA
  ANZAHL_GABA = 1        //Anzahl der synaptischen Pulse
  G_GABA = 0.000789 * 2000    //This Value was pultiplished for the analysis 
  P_GABA = 0.18
  DECAY_GABA = 37  
  GABA_SYN_LOCATION_1 = 0.5  // Position GABA Synapse for Pulses
  GABA_SYN_LOCATION_2 = 0.25  // Position GABA Synapse for Pulses
  GABA_SYN_LOCATION_3 = 0.75  // Position GABA Synapse for Pulses
  ONSET_PULSE_1 = 100
  ONSET_PULSE_2 = 600
  ONSET_PULSE_3 = 600  


//----- Insert synapses -------------------------
// Determination of Synapses
  objref gabasyn1, gabasyn2, gabasyn3 
  dend {
    // insert GABA synapse 
    gabasyn1 = new gaba(GABA_SYN_LOCATION_1)
    gabasyn2 = new gaba(GABA_SYN_LOCATION_2)
    gabasyn3 = new gaba(GABA_SYN_LOCATION_3)
    gabasyn1.tau1 = 0.1
    gabasyn1.tau2 = DECAY_GABA 
    gabasyn2.P = P_GABA
    gabasyn2.tau1 = 0.1
    gabasyn2.tau2 = DECAY_GABA 
    gabasyn3.P = P_GABA
    gabasyn3.tau1 = 0.1
    gabasyn3.tau2 = DECAY_GABA 
    gabasyn3.P = P_GABA
  }

// Definition of synaptic Stimuli
  objref stimGABApuls1, stimGABApuls2, stimGABApuls3  //Pulssequenz GABA
  stimGABApuls1 = new NetStim(GABA_SYN_LOCATION_1)
  stimGABApuls1.number = ANZAHL_GABA
  stimGABApuls1.start = ONSET_PULSE_1
  stimGABApuls2 = new NetStim(GABA_SYN_LOCATION_2)
  stimGABApuls2.number = ANZAHL_GABA
  stimGABApuls2.start = ONSET_PULSE_2
  stimGABApuls3 = new NetStim(GABA_SYN_LOCATION_3)
  stimGABApuls3.number = ANZAHL_GABA
  stimGABApuls3.start = ONSET_PULSE_3

// Linkage of synaptic Inputs
  objref synpulsegaba1,synpulsegaba2, synpulsegaba3 
  synpulsegaba1 = new NetCon(stimGABApuls1, gabasyn1, 0, 0, G_GABA)
  synpulsegaba2 = new NetCon(stimGABApuls2, gabasyn2, 0, 0, G_GABA)
  synpulsegaba3 = new NetCon(stimGABApuls3, gabasyn3, 0, 0, G_GABA)