This is the readme for the model associated with the paper:
Donohue DE, Ascoli GA (2008) A comparative computer simulation of
dendritic morphology. PLoS Comput Biol 4:e1000089
Model descriptions and directions.
Overview:
Two closely related models for creating virtual branching structures
based on 3D reconstructions of real neurons. The models take as input
a parameter file (CA1pA_Amaral23Example.prn) which gives the file path
of several neuronal reconstructions in .swc format. Five basic
parameters are measured from each branch of the input neurons;
branching probability, taper rate, daughter ratio, parent daughter
ratio, and branch length. They are then organized and reduced to
statistical distributions based on three fundamental determinants
(FDs); radius, path distance from the soma, and branch order. The
distributions are then placed into a table file. Morphometrics are
resampled from the tables based on the current virtual fundamental
determinant values to create virtual 2D branching structures.
Model variants:
There are two different model variants. They differ in the way in
which they use three FDs in combination to create virtual trees. The
percent mix variant determines what basic parameter will be sampled by
allowing each fundamental determinant to exert a fraction of the
influence for all of the basic parameters. For example, the sampling
of each basic parameter could be based 30% on branch order, 20% on
path distance, and 50% on radius. The percent step by which the
influence of the three FDs is varied can be adjusted in the .prn file.
The default is 10% steps, leading to 66 unique variations of the
model.
In the parameter mixing variant each basic parameter can be controlled
by a separate FD. Given that there are 5 basic parameters and 3 FDs,
there are 5^3, or 243 unique variants of this model. Note that the
three cases where all five parameters are under the control of the
same FD are equivalent to the three percent mix cases where one FD has
100% influence (although due to differences in random number
generation they are only statistically identical and will not provide
the exact same results). In either case these are referred to as
"pure" models.
Running the program:
The models are designed to be executed from the command line. The
format (in mswin) is:
Java lnded2_0 inputFileName OutputFilePostfix
Where lnded2_0 is the .class file created from the .java files
included in each directory. The input file is a text file with the
extension of .prn in our examples. See the ExampleCommandLine.bat
file for a specific example. When the program is run a table file
prefixed "Table_" is created which gives the statistical distributions
of the basic parameters binned by FDs. For each bin a distribution
type (Uniform (1), Gaussian (2), and Gamma (3)) is chosen based on a
least mean squares matching between the real data and generated data
of each distribution type. An empty file is "OutputInfo_ " file is
also created. If the "DEBUGGING" switch is set to "true" in the .prn
file (explained below) this "OutputInfo_" file will be filled with all
of the raw data used to create the table file. Finally, four .csv
files are created beginning with "VirtAsymetry_", "VirtBifs_",
"VirtSurface_", and "VirtSurfaceAsym_". These contain the group means
and standard deviations for bifurcation asymmetry, bifurcation
numbers, surface area, and surface area asymmetry of the virtual trees
for each model variant. The mean and standard deviation of the given
emergent morphometric for the real input trees is included at the end
of the file.
.prm file options:
The following are the options which can be adjusted through the
parameter file. For each option the value to be assigned is placed on
the following line of the .prm file. Unless otherwise noted all
options apply to both model variants.
INPUT
Takes an integer value referring to the number of .swc files
which are to be loaded. On subsequent individual lines the
full file path of each input files is given. A large number
of .swc files are available at NeuroMorpho.org.
TODO
Takes an integer value giving the number of group of virtual
trees to create. If for example there were 12 input .swc
files with three trees each and TODO is set to 10 (the default
value) then 10 groups of 36 virtual trees will be created.
The mean and standardizations given in the .csv files refer to
theses group means and standardizations.
BINNING
Takes an integer value giving the minimum number of points to
be placed into each FD bin. The default is 85. It is
important to note that this is the minimum and for those FDs
which can be heavily discretized (branch order and radius)
bins may contain many more data points than this. For
example, if there are 50 input branches of branch order 1, 75
of branch order 2, and 130 of branch order three the first bin
will contain 125 data points and the second 130 if the minimum
is set at 85.
TYPETODO
Takes an integer which specifies which tree type to be
analyzed and recreated from the .swc file. The default is 3
(regular or basal dendrite). 4 (apical dendrite) and 2 (axon)
are also accepted as is any custom type value.
MINRAD
Takes a positive double value specifying the minimum allowed
radius. Because the model is stochastic it is possible to
continue reducing the radius values of virtual trees beyond
realistic boundaries. This value specifies the radius values
below which termination is not allowed. Default is .15.
SEED
Takes an integer and sets the random number seed value.
Useful for doing multiple runs without getting the exact same
results each time.
DEBUGGING
Takes either "true" or "false". Boolean which if true
(default) populates the file prefixed with "OutputInfo_" with
many details about the input .swc files parameter values.
SWCOUT
Takes either "true" or "false". Boolean which if true writes
a separate .swc file for each virtual tree created for
analysis by external programs. Because no 2D or 3D
information is included these files will appear as single
lines in visualization software. Default is false.
DENDRO
Takes either "true" or "false". Boolean which if true writes
a separate .swc file for each virtual tree created in the form
of a 2D dendrogram. These files are useful for visualization
and figures, but the lateral offset which separates the
branches adds length to the trees which is not part of the
model. Default is false.
PERCENTSTEP
Takes a double value between 0 and 1. The default of .1 means
that in the percent mix model the various contributions of the
three FDs will be adjusted through all possible combinations
in 10% increments.
