Na+ channel dependence of AP initiation in cortical pyramidal neuron (Kole et al. 2008)

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Accession:114394
In this simulation action potential initiation, action potential properties and the role of axon initial segment Na+ channels are investigated in a realistic model of a layer 5 pyramidal neuron axon initial segment. The main Na+ channel properties were constrained by experimental data and the axon initial segment was reconstructed. Model parameters were constrained by direct recordings at the axon initial segment.
Reference:
1 . Kole MH, Ilschner SU, Kampa BM, Williams SR, Ruben PC, Stuart GJ (2008) Action potential generation requires a high sodium channel density in the axon initial segment. Nat Neurosci 11:178-86 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism:
Cell Type(s): Neocortex V1 pyramidal corticothalamic L6 cell;
Channel(s): I Na,t; I K; I M; I h;
Gap Junctions:
Receptor(s):
Gene(s): HCN1; HCN2;
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s): Action Potential Initiation; Active Dendrites; Axonal Action Potentials; Action Potentials;
Implementer(s): Kole, Maarten [m.kole at nin.knaw.nl];
Search NeuronDB for information about:  Neocortex V1 pyramidal corticothalamic L6 cell; I Na,t; I K; I M; I h;
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NN_kole
readme.html
h.mod
Km.mod *
kv.mod *
Kv1.mod
na.mod *
nax.mod *
A3+AIS_original.hoc
figure6a.hoc
initiate_neuron.hoc
mosinit.hoc
panels.ses
screenshot.jpg
screenshot_adjust.jpg
screenshot_morphology.jpg
                            
objectvar save_window_, rvp_
objectvar scene_vector_[5]
objectvar ocbox_, ocbox_list_, scene_, scene_list_
{ocbox_list_ = new List()  scene_list_ = new List()}
{
xpanel("sodium channel densities", 0)
na_segment = 3000
xvalue("gNa AIS (pS/um2)","na_segment", 1," init_channels()", 0, 0 )
na_soma = 60
xvalue("gNa Somato-Dendritic (pS/um2)","na_soma", 1," init_channels()", 0, 0 )
xpanel(853,83)
}
{
save_window_ = new Graph(0)
save_window_.size(-112.45,321.05,-74.5833,37.5833)
scene_vector_[2] = save_window_
{save_window_.view(-112.45, -74.5833, 433.5, 112.167, 751, 451, 412, 280)}
flush_list.append(save_window_)
save_window_.save_name("flush_list.")
objectvar rvp_
rvp_ = new RangeVarPlot("v")
apic[44] rvp_.begin(1)
myelin[26] rvp_.end(0)
rvp_.origin(21.2434)
save_window_.addobject(rvp_, 2, 1, 0.8, 0.9)
save_window_.label(54.063, 8.56149, "AIS", 1, 1, 0, 0, 3)
save_window_.label(63.6593, -6.28649, "^", 1, 1, 0, 0, 3)
save_window_.label(0.262136, 0.767857, "", 2, 1, 0, 0, 1)
save_window_.label(106.84, 9.1325, "1st Node", 1, 1, 0, 0, 4)
save_window_.label(137.225, -6.28649, "^", 1, 1, 0, 0, 4)
}
{
save_window_ = new Graph(0)
save_window_.size(296.846,315.004,-80.5083,38.4087)
scene_vector_[3] = save_window_
{save_window_.view(296.846, -80.5083, 18.1584, 118.917, 348, 448, 389, 282)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("v(.5)", 1, 1, 0.8, 0.9, 2)
save_window_.addvar("axon.v(0.725)", 3, 1, 0.8, 0.9, 2)
}
{
save_window_ = new PlotShape(0)
save_window_.size(-928.583,1175.59,-1256.29,1137.3)
save_window_.variable("v")
scene_vector_[4] = save_window_
{save_window_.view(-928.583, -1256.29, 2104.17, 2393.59, 60, 451, 269, 306)}
fast_flush_list.append(save_window_)
save_window_.save_name("fast_flush_list.")
}
{
xpanel("RunControl", 0)
v_init = -88
xvalue("Init","v_init", 1,"stdinit()", 1, 1 )
xbutton("Init & Run","run()")
xbutton("Stop","stoprun=1")
runStopAt = 5
xvalue("Continue til","runStopAt", 1,"{continuerun(runStopAt) stoprun=1}", 1, 1 )
runStopIn = 0.01
xvalue("Continue for","runStopIn", 1,"{continuerun(t + runStopIn) stoprun=1}", 1, 1 )
xbutton("Single Step","steprun()")
t = 304.46
xvalue("t","t", 2 )
tstop = 312
xvalue("Tstop","tstop", 1,"tstop_changed()", 0, 1 )
dt = 0.01
xvalue("dt","dt", 1,"setdt()", 0, 1 )
steps_per_ms = 100
xvalue("Points plotted/ms","steps_per_ms", 1,"setdt()", 0, 1 )
screen_update_invl = 0.05
xvalue("Scrn update invl","screen_update_invl", 1,"", 0, 1 )
realtime = 0.0799999
xvalue("Real Time","realtime", 0,"", 0, 1 )
xpanel(64,85)
}
objectvar scene_vector_[1]
{doNotify()}

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