Cerebellar Nucleus Neuron (Steuber, Schultheiss, Silver, De Schutter & Jaeger, 2010)

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Accession:136175
This is the GENESIS 2.3 implementation of a multi-compartmental deep cerebellar nucleus (DCN) neuron model with a full dendritic morphology and appropriate active conductances. We generated a good match of our simulations with DCN current clamp data we recorded in acute slices, including the heterogeneity in the rebound responses. We then examined how inhibitory and excitatory synaptic input interacted with these intrinsic conductances to control DCN firing. We found that the output spiking of the model reflected the ongoing balance of excitatory and inhibitory input rates and that changing the level of inhibition performed an additive operation. Rebound firing following strong Purkinje cell input bursts was also possible, but only if the chloride reversal potential was more negative than -70 mV to allow de-inactivation of rebound currents. Fast rebound bursts due to T-type calcium current and slow rebounds due to persistent sodium current could be differentially regulated by synaptic input, and the pattern of these rebounds was further influenced by HCN current. Our findings suggest that active properties of DCN neurons could play a crucial role for signal processing in the cerebellum.
Reference:
1 . Steuber V, Schultheiss NW, Silver RA, De Schutter E, Jaeger D (2011) Determinants of synaptic integration and heterogeneity in rebound firing explored with data-driven models of deep cerebellar nucleus cells. J Comput Neurosci 30:633-58 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism: Cerebellum;
Cell Type(s): Cerebellum deep nucleus neuron;
Channel(s): I Na,p; I T low threshold; I h;
Gap Junctions:
Receptor(s): GabaA; AMPA; NMDA;
Gene(s):
Transmitter(s): Gaba; Glutamate;
Simulation Environment: GENESIS;
Model Concept(s): Bursting; Ion Channel Kinetics; Active Dendrites; Detailed Neuronal Models; Intrinsic plasticity; Rate-coding model neurons; Synaptic Integration; Rebound firing;
Implementer(s): Steuber, Volker [v.steuber at herts.ac.uk]; Jaeger, Dieter [djaeger at emory.edu];
Search NeuronDB for information about:  GabaA; AMPA; NMDA; I Na,p; I T low threshold; I h; Gaba; Glutamate;
//genesis

function make_cn_comps

  float shell_vol

  echo making CN compartment library...
  
  // soma prototype
  if (!({exists CN_soma}))
    create compartment CN_soma
  end

  setfield CN_soma Cm {{CM}*soma_area} Ra {8.0*{RA}/(soma_d*{PI})} \
     		   Em {ELEAK} initVm {EREST_ACT} Rm {{RMs}/soma_area} inject 0.0 \
     		   dia {soma_d} len {soma_l}
  shell_vol = {PI}/3.0*(3.0*soma_d*soma_d*shell_thick - 6.0*soma_d*shell_thick*shell_thick + 4.0*shell_thick*shell_thick*shell_thick)

  // add currents to soma prototype
  copy GHK CN_soma/GHKs
  addmsg CN_soma CN_soma/GHKs VOLTAGE Vm
  addmsg CN_soma/GHKs CN_soma CHANNEL Gk Ek			

  copy NaF CN_soma/NaFs
  addmsg CN_soma CN_soma/NaFs VOLTAGE Vm
  addmsg CN_soma/NaFs CN_soma CHANNEL Gk Ek
  setfield CN_soma/NaFs Gbar {soma_area*{GNaFs}}	
  
  copy NaP CN_soma/NaPs
  addmsg CN_soma CN_soma/NaPs VOLTAGE Vm
  addmsg CN_soma/NaPs CN_soma CHANNEL Gk Ek
  setfield CN_soma/NaPs Gbar {soma_area*{GNaPs}}
  
  copy TNC CN_soma/TNCs
  addmsg CN_soma CN_soma/TNCs VOLTAGE Vm
  addmsg CN_soma/TNCs CN_soma CHANNEL Gk Ek
  setfield CN_soma/TNCs Gbar {soma_area*{GTNCs}}	

  copy fKdr CN_soma/fKdrs
  addmsg CN_soma CN_soma/fKdrs VOLTAGE Vm
  addmsg CN_soma/fKdrs CN_soma CHANNEL Gk Ek
  setfield CN_soma/fKdrs Gbar {soma_area*{GfKdrs}}	

  copy sKdr CN_soma/sKdrs
  addmsg CN_soma CN_soma/sKdrs VOLTAGE Vm
  addmsg CN_soma/sKdrs CN_soma CHANNEL Gk Ek
  setfield CN_soma/sKdrs Gbar {soma_area*{GsKdrs}}	

  copy Sk CN_soma/Sks
  addmsg CN_soma/Sks CN_soma CHANNEL Gk Ek
  setfield CN_soma/Sks Gbar {soma_area*{GSks}}	

  copy h_slow CN_soma/h_slows
  addmsg CN_soma CN_soma/h_slows VOLTAGE Vm
  addmsg CN_soma/h_slows CN_soma CHANNEL Gk Ek
  setfield CN_soma/h_slows Gbar {soma_area*{Ghs}}	

  copy CaLVA CN_soma/CaLVAs
  addmsg CN_soma CN_soma/CaLVAs VOLTAGE Vm
  setfield CN_soma/CaLVAs Gbar {soma_area*{GCaLVAs}}
  addmsg CN_soma/CaLVAs CN_soma CHANNEL Gk Ek 

  copy CaHVA CN_soma/CaHVAs
  addmsg CN_soma CN_soma/CaHVAs VOLTAGE Vm
  setfield CN_soma/CaHVAs Gbar {soma_area*{GCaHVAs}}	
  addmsg CN_soma/CaHVAs CN_soma/GHKs ADD_GBAR Gk 

  create Ca_concen CN_soma/Ca_pool
  setfield CN_soma/Ca_pool tau {catau} \
                           B {{kCas}/{shell_vol}} \
                           Ca_base {CCaI} \
                           thick {shell_thick}
  addmsg CN_soma/GHKs CN_soma/Ca_pool I_Ca Ik
  addmsg CN_soma/Ca_pool CN_soma/GHKs CONCEN1 Ca
  addmsg CN_soma/Ca_pool CN_soma/Sks CONCEN Ca
  

  // axon hillock prototype
  if (!({exists CN_axHill}))
        create compartment CN_axHill
  end
  setfield CN_axHill Cm {{CM}*axon_area} Ra {4.0*{RA}*axon_l/(axon_d*axon_d*{PI})} \
     Em {ELEAK} initVm {EREST_ACT} Rm {{RMs}/axon_area} inject 0.0 \
     dia {axon_d} len {axon_l}
  shell_vol = {PI}*axon_l*(axon_d*shell_thick - shell_thick*shell_thick)

  // add currents to axon hillock prototype - initially only NaF and fKdr
  copy NaF CN_axHill/NaFa
  addmsg CN_axHill CN_axHill/NaFa VOLTAGE Vm
  addmsg CN_axHill/NaFa CN_axHill CHANNEL Gk Ek
  setfield CN_axHill/NaFa Gbar {axon_area*{GNaFaxHill}}	

  copy TNC CN_axHill/TNCa
  addmsg CN_axHill CN_axHill/TNCa VOLTAGE Vm
  addmsg CN_axHill/TNCa CN_axHill CHANNEL Gk Ek
  setfield CN_axHill/TNCa Gbar {axon_area*{GTNCaxHill}}	

  copy fKdr CN_axHill/fKdra
  addmsg CN_axHill CN_axHill/fKdra VOLTAGE Vm
  addmsg CN_axHill/fKdra CN_axHill CHANNEL Gk Ek
  setfield CN_axHill/fKdra Gbar {axon_area*{GfKdraxHill}}	

  copy sKdr CN_axHill/sKdra
  addmsg CN_axHill CN_axHill/sKdra VOLTAGE Vm
  addmsg CN_axHill/sKdra CN_axHill CHANNEL Gk Ek
  setfield CN_axHill/sKdra Gbar {axon_area*{GsKdraxHill}}	

  // axon initial segment prototype
  if (!({exists CN_axIS}))
        create compartment CN_axIS
  end
  setfield CN_axIS Cm {{CM}*axon_area} Ra {4.0*{RA}*axon_l/(axon_d*axon_d*{PI})} \
     Em {ELEAKax} initVm {EREST_ACT} Rm {{RMax}/axon_area} inject 0.0 \
     dia {axon_d} len {axon_l}
  shell_vol = {PI}*axon_l*(axon_d*shell_thick - shell_thick*shell_thick)

  // add currents - initially only NaF and fKdr
  copy NaF CN_axIS/NaFa
  addmsg CN_axIS CN_axIS/NaFa VOLTAGE Vm
  addmsg CN_axIS/NaFa CN_axIS CHANNEL Gk Ek
  setfield CN_axIS/NaFa Gbar {axon_area*{GNaFaxIS}}	

  copy TNC CN_axIS/TNCa
  addmsg CN_axIS CN_axIS/TNCa VOLTAGE Vm
  addmsg CN_axIS/TNCa CN_axIS CHANNEL Gk Ek
  setfield CN_axIS/TNCa Gbar {axon_area*{GTNCaxIS}}	

  copy fKdr CN_axIS/fKdra
  addmsg CN_axIS CN_axIS/fKdra VOLTAGE Vm
  addmsg CN_axIS/fKdra CN_axIS CHANNEL Gk Ek
  setfield CN_axIS/fKdra Gbar {axon_area*{GfKdraxIS}}

  copy sKdr CN_axIS/sKdra
  addmsg CN_axIS CN_axIS/sKdra VOLTAGE Vm
  addmsg CN_axIS/sKdra CN_axIS CHANNEL Gk Ek
  setfield CN_axIS/sKdra Gbar {axon_area*{GsKdraxIS}}

  // axon internodal segment prototype - no channels
  if (!({exists CN_axIN}))
        create compartment CN_axIN
  end
  setfield CN_axIN Cm {{CMmy}*axon_area} Ra {4.0*{RA}*axon_l/(axon_d*axon_d*{PI})} \
     Em {ELEAKax} initVm {EREST_ACT} Rm {{RMmy}/axon_area} inject 0.0 \
     dia {axon_d} len {axon_l}
  shell_vol = {PI}*axon_l*(axon_d*shell_thick - shell_thick*shell_thick)

  
  // proximal dendrite prototype
  if (!({exists CN_pdend}))
        create compartment CN_pdend
  end
  setfield CN_pdend Cm {{CM}*dend_area} Ra {4.0*{RA}*dend_l/(dend_d*dend_d*{PI})} \
     Em {ELEAK} initVm {EREST_ACT} Rm {{RMd}/dend_area} inject 0.0 \
     dia {dend_d} len {dend_l}
  shell_vol = {PI}*dend_l*(dend_d*shell_thick - shell_thick*shell_thick)
  
  // first copy proximal to distal dendrite prototype
  if (!({exists CN_ddend}))
        copy CN_pdend CN_ddend 
  end

  // add currents to proximal dendrite prototype
  copy GHK CN_pdend/GHKpd
  addmsg CN_pdend CN_pdend/GHKpd VOLTAGE Vm
  addmsg CN_pdend/GHKpd CN_pdend CHANNEL Gk Ek			

  copy NaF CN_pdend/NaFpd
  addmsg CN_pdend CN_pdend/NaFpd VOLTAGE Vm
  addmsg CN_pdend/NaFpd CN_pdend CHANNEL Gk Ek
  setfield CN_pdend/NaFpd Gbar {dend_area*{GNaFpd}}	

  copy TNC CN_pdend/TNCpd
  addmsg CN_pdend CN_pdend/TNCpd VOLTAGE Vm
  addmsg CN_pdend/TNCpd CN_pdend CHANNEL Gk Ek
  setfield CN_pdend/TNCpd Gbar {dend_area*{GTNCpd}}	

  copy NaP CN_pdend/NaPpd
  addmsg CN_pdend CN_pdend/NaPpd VOLTAGE Vm
  addmsg CN_pdend/NaPpd CN_pdend CHANNEL Gk Ek
  setfield CN_pdend/NaPpd Gbar {dend_area*{GNaPpd}}	

  copy fKdr CN_pdend/fKdrpd
  addmsg CN_pdend CN_pdend/fKdrpd VOLTAGE Vm
  addmsg CN_pdend/fKdrpd CN_pdend CHANNEL Gk Ek
  setfield CN_pdend/fKdrpd Gbar {dend_area*{GfKdrpd}}	

  copy sKdr CN_pdend/sKdrpd
  addmsg CN_pdend CN_pdend/sKdrpd VOLTAGE Vm
  addmsg CN_pdend/sKdrpd CN_pdend CHANNEL Gk Ek
  setfield CN_pdend/sKdrpd Gbar {dend_area*{GsKdrpd}}	

  copy Sk CN_pdend/Skpd
  addmsg CN_pdend/Skpd CN_pdend CHANNEL Gk Ek
  setfield CN_pdend/Skpd Gbar {dend_area*{GSkpd}}	

  copy h_slow CN_pdend/h_slowpd
  addmsg CN_pdend CN_pdend/h_slowpd VOLTAGE Vm
  addmsg CN_pdend/h_slowpd CN_pdend CHANNEL Gk Ek
  setfield CN_pdend/h_slowpd Gbar {dend_area*{Ghpd}}	

  copy CaLVA CN_pdend/CaLVApd
  addmsg CN_pdend CN_pdend/CaLVApd VOLTAGE Vm
  setfield CN_pdend/CaLVApd Gbar {dend_area*{GCaLVApd}}	
  addmsg CN_pdend/CaLVApd CN_pdend CHANNEL Gk Ek 

  copy CaHVA CN_pdend/CaHVApd
  addmsg CN_pdend CN_pdend/CaHVApd VOLTAGE Vm
  setfield CN_pdend/CaHVApd Gbar {dend_area*{GCaHVApd}}	
  addmsg CN_pdend/CaHVApd CN_pdend/GHKpd ADD_GBAR Gk 

  create Ca_concen CN_pdend/Ca_pool
  setfield CN_pdend/Ca_pool tau {catau} \
                              B {{kCad}/{shell_vol}} \
                              Ca_base {CCaI} \
                              thick {shell_thick}
  addmsg CN_pdend/GHKpd CN_pdend/Ca_pool I_Ca Ik 
  addmsg CN_pdend/Ca_pool CN_pdend/GHKpd CONCEN1 Ca
  addmsg CN_pdend/Ca_pool CN_pdend/Skpd CONCEN Ca
 

  // add currents to distal dendrite prototype
  copy GHK CN_ddend/GHKdd
  addmsg CN_ddend CN_ddend/GHKdd VOLTAGE Vm
  addmsg CN_ddend/GHKdd CN_ddend CHANNEL Gk Ek			

  copy NaF CN_ddend/NaFdd
  addmsg CN_ddend CN_ddend/NaFdd VOLTAGE Vm
  addmsg CN_ddend/NaFdd CN_ddend CHANNEL Gk Ek
  setfield CN_ddend/NaFdd Gbar {dend_area*{GNaFdd}}	

  copy TNC CN_ddend/TNCdd
  addmsg CN_ddend CN_ddend/TNCdd VOLTAGE Vm
  addmsg CN_ddend/TNCdd CN_ddend CHANNEL Gk Ek
  setfield CN_ddend/TNCdd Gbar {dend_area*{GTNCdd}}	

  copy NaP CN_ddend/NaPdd
  addmsg CN_ddend CN_ddend/NaPdd VOLTAGE Vm
  addmsg CN_ddend/NaPdd CN_ddend CHANNEL Gk Ek
  setfield CN_ddend/NaPdd Gbar {dend_area*{GNaPdd}}	

  copy fKdr CN_ddend/fKdrdd
  addmsg CN_ddend CN_ddend/fKdrdd VOLTAGE Vm
  addmsg CN_ddend/fKdrdd CN_ddend CHANNEL Gk Ek
  setfield CN_ddend/fKdrdd Gbar {dend_area*{GfKdrdd}}	

  copy Sk CN_ddend/Skdd
  addmsg CN_ddend/Skdd CN_ddend CHANNEL Gk Ek
  setfield CN_ddend/Skdd Gbar {dend_area*{GSkdd}}	

  copy h_slow CN_ddend/h_slowdd
  addmsg CN_ddend CN_ddend/h_slowdd VOLTAGE Vm
  addmsg CN_ddend/h_slowdd CN_ddend CHANNEL Gk Ek
  setfield CN_ddend/h_slowdd Gbar {dend_area*{Ghdd}}	

  copy CaLVA CN_ddend/CaLVAdd
  addmsg CN_ddend CN_ddend/CaLVAdd VOLTAGE Vm
  setfield CN_ddend/CaLVAdd Gbar {dend_area*{GCaLVAdd}}	
  addmsg CN_ddend/CaLVAdd CN_ddend CHANNEL Gk Ek  

  copy CaHVA CN_ddend/CaHVAdd
  addmsg CN_ddend CN_ddend/CaHVAdd VOLTAGE Vm
  setfield CN_ddend/CaHVAdd Gbar {dend_area*{GCaHVAdd}}	
  addmsg CN_ddend/CaHVAdd CN_ddend/GHKdd ADD_GBAR Gk 

  create Ca_concen CN_ddend/Ca_pool
  setfield CN_ddend/Ca_pool tau {catau} \
                              B {{kCad}/{shell_vol}} \
                              Ca_base {CCaI} \
                              thick {shell_thick}
  addmsg CN_ddend/GHKdd CN_ddend/Ca_pool I_Ca Ik
  addmsg CN_ddend/Ca_pool CN_ddend/GHKdd CONCEN1 Ca
  addmsg CN_ddend/Ca_pool CN_ddend/Skdd CONCEN Ca

  echo done.

end

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