CA1 pyramidal neuron: depolarization block (Bianchi et al. 2012)

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Accession:143719
NEURON files from the paper: On the mechanisms underlying the depolarization block in the spiking dynamics of CA1 pyramidal neurons by D.Bianchi, A. Marasco, A.Limongiello, C.Marchetti, H.Marie,B.Tirozzi, M.Migliore (2012). J Comput. Neurosci. In press. DOI: 10.1007/s10827-012-0383-y. Experimental findings shown that under sustained input current of increasing strength neurons eventually stop firing, entering a depolarization block. We analyze the spiking dynamics of CA1 pyramidal neuron models using the same set of ionic currents on both an accurate morphological reconstruction and on its reduction to a single-compartment. The results show the specic ion channel properties and kinetics that are needed to reproduce the experimental findings, and how their interplay can drastically modulate the neuronal dynamics and the input current range leading to depolarization block.
Reference:
1 . Bianchi D, Marasco A, Limongiello A, Marchetti C, Marie H, Tirozzi B, Migliore M (2012) On the mechanisms underlying the depolarization block in the spiking dynamics of CA1 pyramidal neurons. J Comput Neurosci 33:207-25 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism: Hippocampus;
Cell Type(s): Hippocampus CA1 pyramidal cell;
Channel(s): I Na,t; I A; I K; I M; I h; I K,Ca; I_AHP;
Gap Junctions:
Receptor(s): GabaA; AMPA; NMDA;
Gene(s):
Transmitter(s): Gaba; Glutamate;
Simulation Environment: NEURON; Mathematica;
Model Concept(s): Simplified Models; Depolarization block; Bifurcation;
Implementer(s): Bianchi, Daniela [danielabianchi12 -at- gmail.com]; Limongiello, Alessandro [alessandro.limongiello at unina.it];
Search NeuronDB for information about:  Hippocampus CA1 pyramidal cell; GabaA; AMPA; NMDA; I Na,t; I A; I K; I M; I h; I K,Ca; I_AHP; Gaba; Glutamate;
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Ca1_Bianchi
experiment
cad.mod *
cagk.mod *
cal.mod *
calH.mod *
car.mod *
cat.mod *
d3.mod *
h.mod *
kadist.mod *
kaprox.mod *
kca.mod *
kdr.mod *
km.mod *
na3.mod *
na3dend.mod *
na3notrunk.mod *
nap.mod *
nax.mod *
somacar.mod *
cell-setup.hoc
mosinit.hoc
sessio.ses
Simulation.hoc
                            

//////// load needed templates////////////
load_file("nrngui.hoc")
load_file("../template/load_templates.hoc")

objref econ                                                                  
show_errs=1
debug_lev=1
econ=new ExperimentControl(show_errs,debug_lev)
econ.self_define(econ)

econ.morphology_dir = "../morphology/n123"       // set location for morphology files
econ.add_lib_dir("Terrence","../lib")                     // set location for library files
econ.generic_dir    = "../experiment"             // set location for cell-setup file 

v_init=-70

econ.xopen_geometry_dependent("cell")                                        // load raw cell morphology
econ.xopen_geometry_dependent("cell-analysis")                               // load user-defined semantics on morphology
cell_analysis(econ)
printf("Opening cell setup\n")                                               // load cell-setup to
econ.xopen_generic("cell-setup")                                             // specify all mechanisms,
printf("Opened. Setting up cell\n")                                          // membrane properties etc
cell_setup(econ)

///////////////////Load File//////////////////////

load_file("sessio.ses")
cvode_active(1)

//////   Spike counter//////////

objref apc, v1

proc insert_APC() {
apc = new APCount(0.5)
apc.thresh = $1
v1 = new Vector()
apc.record(v1)
}

//////    IClamp    ///////
objectvar clamp
proc insert_IClamp() {
     clamp = new IClamp(0.5)
     clamp.del = $3
     clamp.dur = $1
     clamp.amp = $2
}



            
////////////////////Main///////////////////


proc main(){

  del=100
  tstop=1000+del+200
  tstopI=1000
  
  in=0
  
  th=-20
  access soma
  insert_APC(th)
  
  for u= in,15{

 
current=u*0.1
access soma
insert_IClamp(tstopI,current,del)

//////////////run//////////////////////// 
 finitialize(v_init)
 fcurrent()
 run()
/////////////////////////////////////
 
  // print the spike number 
  printf("\n Current: %.2f nA | # Spikes: %d\n", current,  apc.n)
  printf("___________________\n")
   
}   // for u

}

main()
/********    end file    ******/


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