Leech heart interneuron network model (Hill et al 2001, 2002)

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Accession:19698
We have created a computational model of the timing network that paces the heartbeat of the medicinal leech, Hirudo medicinalis. In the intact nerve cord, segmental oscillators are mutually entrained to the same cycle period. Although experiments have shown that the segmental oscillators are coupled by inhibitory coordinating interneurons, the underlying mechanisms of intersegmental coordination have not yet been elucidated. To help understand this coordination, we have created a simple computational model with two variants: symmetric and asymmetric. See references for more details. Biologically realistic network models with two, six, and eight cells and a tutorial are available at the links to Calabrese's web site below.
References:
1 . Hill AA, Masino MA, Calabrese RL (2002) Model of intersegmental coordination in the leech heartbeat neuronal network. J Neurophysiol 87:1586-602 [PubMed]
2 . Hill AA, Lu J, Masino MA, Olsen OH, Calabrese RL (2001) A model of a segmental oscillator in the leech heartbeat neuronal network. J Comput Neurosci 10:281-302 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Realistic Network;
Brain Region(s)/Organism: Leech;
Cell Type(s): Leech heart interneuron;
Channel(s): I Na,p; I Na,t; I K; I h; I Calcium; I Potassium;
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: GENESIS;
Model Concept(s): Activity Patterns; Bursting; Oscillations; Spatio-temporal Activity Patterns; Tutorial/Teaching; Action Potentials; Invertebrate;
Implementer(s): Hill, Andrew A [aavhill at yahoo.com];
Search NeuronDB for information about:  I Na,p; I Na,t; I K; I h; I Calcium; I Potassium;
This directory contains source code for two new libraries that need to be added
to your GENESIS executable in order to use the network leech model.  These are
meant to be added to an already-compiled version of GENESIS, so you will _not_
need to recompile your GENESIS program; you'll just be creating a new GENESIS
program from your already-compiled version.

*  If you use a common machine, such as a Sun or a Linux machine, check to
   make sure we haven't placed a pre-compiled version on our ftp site.  It
   might save you some time to get a pre-compiled version.

To add the libraries, do the following:

1)  copy Makefile.usermake from the GENESIS/Scripts/newlib directory this
    directory and name it Makefile.

2)  Follow the directions in newly copied Makefile, such as setting the machine
    type, setting your compiler,  and giving the new executable a name.

3)  Also within Makefile, set the following:

     USERDIR	= newlib1 newlib2

     USEROBJ	= newlib1/newconnlib.o newlib2/leechlib.o

     USERLIB	= newconnlib leech

4)  Edit the line "GENESIS = /usr/local/genesis" in both Makefile and
    newlib1/Makefile so it points to your GENESIS installation directory.

5)  Make sure you have done a "make install" in the GENESIS/src directory.  (It
    is possible to use GENESIS without doing this, but this Makefile assumes
    that you have put a copy of each library in GENESIS/lib.)

6)  If you use any libraries that are not in the standard GENESIS distribution
    (such as spikelib; look for GENESIS/lib/spikelib to see if you have it), 
    add them to the "GENESIS" and "LIBLIST" variables in the Makefile.

7)  Type "make".

This directory contains an example Makefile file for Linux.  It includes the
spike library and uses GNU development tools.  (cc maps to gcc on our system.)

Questions?  Email andrew@biology.emory.edu or vanhoosr@biology.emory.edu.


P.S. In order for the leech model neurons to oscillate properly, the state 
variables must be set to appropriate intial values.  This may be done with the
genesis restore command which saves the necessary state variables of a 
simulation in a binary format.  Unfortunately a binary file created on one
machine may not work on another.  To avoid this problem, we restore the values
of the state variables  using a series of setfield commands.  If you press the
INIT button in the simulations this will cause this series of setfield 
commands to be executed.  There are a few state variables in the Synchan object
that are readonly in the standard genesis distribution.  Trying to set the 
fields of these variables will lead to error messages.  To avoid these    
errors copy the file newconn.g found in this directory to the 
/genesis/src/newconn directory.  This will overwrite the existing newconn.  
Alternatively, simply edit the newconn.g file to make Ik, Gk, X, and Y 
readwrite.  Genesis will have to be recomplied in order for these changes to 
take effect.  Another way to work around this problem is to inject a current
pulse into one of the cells (e.g. -.1 nA for 5 sec) to help the cells begin to 
oscillate and then use the binary save option to save a new set of initial 
state variables.










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