Pyramidal neurons with mutated SCN2A gene (Nav1.2) (Ben-Shalom et al 2017)

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Accession:223955
Model of pyramidal neurons that either hyper or hypo excitable due to SCN2A mutations. Mutations are taken from patients with ASD or Epilepsy
Reference:
1 . Ben-Shalom R, Keeshen CM,Berrios KN, An JY, Sanders SJ, Bender KJ (2017) Opposing effects on NaV1.2 function underlie differences between SCN2A variants observed in individuals with autism spectrum disorder or infantile seizures Biological Psychiatry [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism:
Cell Type(s): Neocortex V1 pyramidal corticothalamic L6 cell;
Channel(s): I Na,t; I Sodium; I K;
Gap Junctions:
Receptor(s):
Gene(s): Nav1.2 SCN2A;
Transmitter(s):
Simulation Environment: NEURON; MATLAB;
Model Concept(s): Epilepsy; Autism spectrum disorder;
Implementer(s): Ben-Shalom, Roy [bens.roy at gmail.com];
Search NeuronDB for information about:  Neocortex V1 pyramidal corticothalamic L6 cell; I Na,t; I K; I Sodium;
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SCN2A_ASD
Excitability
AdultOrig
readme.txt *
Cad.mod *
CaH.mod *
CaT.mod *
charge.mod *
h.mod *
Kca.mod *
Kv.mod *
Kv1_axonal.mod *
Kv7.mod *
na8st.mod *
na8st1.mod *
nax8st.mod *
28_04_10_num19.hoc *
all_28_04_10_num19.ses *
Cell parameters.hoc *
charge.hoc *
mosinit.hoc *
scn2aExps.hoc
                            
{load_file("nrngui.hoc")}
objectvar save_window_, rvp_
objectvar scene_vector_[19]
objectvar ocbox_, ocbox_list_, scene_, scene_list_
{ocbox_list_ = new List()  scene_list_ = new List()}
{pwman_place(707,1065,0)}

//Begin PointProcessManager
{
load_file("pointman.hoc")
}
{
soma ocbox_ = new PointProcessManager(0)
}
{object_push(ocbox_)}
{
mt.select("IClamp") i = mt.selected()
ms[i] = new MechanismStandard("IClamp")
ms[i].set("del", 515, 0)
ms[i].set("dur", 3, 0)
ms[i].set("amp", 2.5, 0)
mt.select("IClamp") i = mt.selected() maction(i)
hoc_ac_ = 0.5
sec.sec move() d1.flip_to(0)
}
{object_pop() doNotify()}
{
ocbox_ = ocbox_.v1
ocbox_.map("PointProcessManager", 270, 82, 220, 325)
}
objref ocbox_
//End PointProcessManager

{
xpanel("RunControl", 0)
v_init = -85
xvalue("Init","v_init", 1,"stdinit()", 1, 1 )
xbutton("Init & Run","run()")
xbutton("Stop","stoprun=1")
runStopAt = 5
xvalue("Continue til","runStopAt", 1,"{continuerun(runStopAt) stoprun=1}", 1, 1 )
runStopIn = 1
xvalue("Continue for","runStopIn", 1,"{continuerun(t + runStopIn) stoprun=1}", 1, 1 )
xbutton("Single Step","steprun()")
t = 540
xvalue("t","t", 2 )
tstop = 540
xvalue("Tstop","tstop", 1,"tstop_changed()", 0, 1 )
dt = 0.01
xvalue("dt","dt", 1,"setdt()", 0, 1 )
steps_per_ms = 100
xvalue("Points plotted/ms","steps_per_ms", 1,"setdt()", 0, 1 )
screen_update_invl = 0.05
xvalue("Scrn update invl","screen_update_invl", 1,"", 0, 1 )
realtime = 52.14
xvalue("Real Time","realtime", 0,"", 0, 1 )
xpanel(11,83)
}
{
xpanel("charge (Globals)", 0)
tStart_charge_ = 517
xvalue("tStart_charge_","tStart_charge_", 1,"", 0, 0 )
tEnd_charge_ = 535
xvalue("tEnd_charge_","tEnd_charge_", 1,"", 0, 0 )
peak_tolerance_charge_ = 0.01
xvalue("peak_tolerance_charge_","peak_tolerance_charge_", 1,"", 0, 0 )
peak_lowest_charge_ = -60
xvalue("peak_lowest_charge_","peak_lowest_charge_", 1,"", 0, 0 )
xpanel(813,34)
}
{
save_window_ = new Graph(0)
save_window_.size(-1600,900,1.2,2.7)
scene_vector_[3] = save_window_
{save_window_.view(-1600, 1.2, 2500, 1.5, 819, 168, 304, 122)}
flush_list.append(save_window_)
save_window_.save_name("flush_list.")
objectvar rvp_
rvp_ = new RangeVarPlot("na_ch_excess_ratio_charge_")
apic[56] rvp_.begin(1)
my[9] rvp_.end(0)
rvp_.origin(17.5097)
save_window_.addobject(rvp_, 1, 1, 0.8, 0.9)
}
{
save_window_ = new Graph(0)
save_window_.size(-1677.25,970.25,0.182,1.222)
scene_vector_[4] = save_window_
{save_window_.view(-1677.25, 0.182, 2647.5, 1.04, 826, 319, 296, 111)}
flush_list.append(save_window_)
save_window_.save_name("flush_list.")
objectvar rvp_
rvp_ = new RangeVarPlot("overl_charge_")
apic[56] rvp_.begin(1)
my[9] rvp_.end(0)
rvp_.origin(17.5097)
save_window_.addobject(rvp_, 1, 1, 0.8, 0.9)
}
{
save_window_ = new Graph(0)
save_window_.size(517,522,-76,2)
scene_vector_[5] = save_window_
{save_window_.view(517, -76, 5, 78, 256, 678, 196, 183)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("apic[18].v(.9)", 1, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(517,524,-74,-30)
scene_vector_[6] = save_window_
{save_window_.view(517, -74, 7, 44, 32, 675, 201, 190)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("apic[36].v(.2)", 1, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(517,524,-0.046,0.002)
scene_vector_[7] = save_window_
{save_window_.view(517, -0.046, 7, 0.048, 32, 446, 199, 200)}
graphList[1].append(save_window_)
save_window_.save_name("graphList[1].")
save_window_.addexpr("apic[36].ina(.2)", 2, 1, 0.8, 0.9, 2)
save_window_.addexpr("apic[36].ik(.2)", 3, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(517,522,-0.76,0.06)
scene_vector_[8] = save_window_
{save_window_.view(517, -0.76, 5, 0.82, 257, 447, 191, 205)}
graphList[1].append(save_window_)
save_window_.save_name("graphList[1].")
save_window_.addexpr("apic[18].ina(.9)", 2, 1, 0.8, 0.9, 2)
save_window_.addexpr("apic[18].ik(.9)", 3, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(516,520,-80,30)
scene_vector_[9] = save_window_
{save_window_.view(516, -80, 4, 110, 484, 686, 199, 174)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("v(.5)", 1, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(516,520,-1.2,0.7)
scene_vector_[10] = save_window_
{save_window_.view(516, -1.2, 4, 1.9, 480, 450, 199, 209)}
graphList[1].append(save_window_)
save_window_.save_name("graphList[1].")
save_window_.addexpr("soma.ina(.5)", 2, 1, 0.8, 0.9, 2)
save_window_.addexpr("soma.ik(.5)", 3, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(516,520,-80,40)
scene_vector_[11] = save_window_
{save_window_.view(516, -80, 4, 120, 712, 685, 188, 172)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("axon[0].v(.9)", 1, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(516,520,-16,8)
scene_vector_[12] = save_window_
{save_window_.view(516, -16, 4, 24, 711, 460, 189, 190)}
graphList[1].append(save_window_)
save_window_.save_name("graphList[1].")
save_window_.addexpr("axon[0].ina(.9)", 2, 1, 0.8, 0.9, 2)
save_window_.addexpr("axon[0].ik(.9)", 3, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(518,522,-100,20)
scene_vector_[13] = save_window_
{save_window_.view(518, -100, 4, 120, 1121, 689, 176, 169)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("axon[8].v(.5)", 1, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(518,522,-1.2,0.8)
scene_vector_[14] = save_window_
{save_window_.view(518, -1.2, 4, 2, 1117, 458, 180, 195)}
graphList[1].append(save_window_)
save_window_.save_name("graphList[1].")
save_window_.addexpr("axon[8].ina(.5)", 2, 1, 0.8, 0.9, 2)
save_window_.addexpr("axon[8].ik(.5)", 3, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(517,521,-100,40)
scene_vector_[15] = save_window_
{save_window_.view(517, -100, 4, 140, 923, 687, 183, 163)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("node[6].v(.5)", 1, 1, 0.8, 0.9, 2)
}
{
save_window_ = new Graph(0)
save_window_.size(517,521,-14,6)
scene_vector_[16] = save_window_
{save_window_.view(517, -14, 4, 20, 923, 461, 178, 194)}
graphList[1].append(save_window_)
save_window_.save_name("graphList[1].")
save_window_.addvar("node[6].ina( 0.5 )", 2, 1, 0.8, 0.9, 2)
save_window_.addexpr("node[6].ik( 0.5 )", 3, 1, 0.8, 0.9, 2)
}
{
ocbox_ = new VBox()
ocbox_list_.prepend(ocbox_)
ocbox_.intercept(1)
}
{
save_window_ = new PlotShape(0)
save_window_.size(-679.903,1036.8,-954.47,1350.14)
save_window_.variable("overl_charge_")
scene_vector_[17] = save_window_
{save_window_.view(-679.903, -954.47, 1716.7, 2304.61, 500, 20, 292, 392)}
fast_flush_list.append(save_window_)
save_window_.save_name("fast_flush_list.")
}
{
ocbox_ = ocbox_list_.object(0)
ocbox_.intercept(0)
ocbox_.map("Shape plot", 500, 20, 300, 400)
}
objref ocbox_
{
ocbox_ = new VBox()
ocbox_list_.prepend(ocbox_)
ocbox_.intercept(1)
}
{
save_window_ = new PlotShape(0)
save_window_.size(-679.903,1036.79,-954.47,1350.14)
save_window_.variable("overl_charge_")
scene_vector_[18] = save_window_
{save_window_.view(-679.903, -954.47, 1716.7, 2304.61, 500, 20, 292, 392)}
fast_flush_list.append(save_window_)
save_window_.save_name("fast_flush_list.")
}
{
ocbox_ = ocbox_list_.object(0)
ocbox_.intercept(0)
ocbox_.map("Shape plot", 500, 20, 300, 400)
}
objref ocbox_
objectvar scene_vector_[1]
{doNotify()}

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