Active dendrites and spike propagation in a hippocampal interneuron (Saraga et al 2003)

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Accession:28316
We create multi-compartment models of an Oriens-Lacunosum/Moleculare (O-LM) hippocampal interneuron using passive properties, channel kinetics, densities and distributions specific to this cell type, and explore its signaling characteristics. We find that spike initiation depends on both location and amount of input, as well as the intrinsic properties of the interneuron. Distal synaptic input always produces strong back-propagating spikes whereas proximal input could produce both forward and back-propagating spikes depending on the input strength. Please see paper for more details.
Reference:
1 . Saraga F, Wu CP, Zhang L, Skinner FK (2003) Active Dendrites and Spike Propagation in Multi-compartment Models of Oriens-Lacunosum/Moleculare Hippocampal Interneurons. J Physiol 552(3):673-689 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism:
Cell Type(s): Hippocampus CA1 interneuron oriens alveus cell;
Channel(s): I Na,t; I A; I K; I h;
Gap Junctions:
Receptor(s): AMPA;
Gene(s):
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s): Action Potential Initiation; Dendritic Action Potentials; Active Dendrites; Influence of Dendritic Geometry; Detailed Neuronal Models; Action Potentials;
Implementer(s): Saraga, Fernanda [Fernanda.Saraga at utoronto.ca];
Search NeuronDB for information about:  Hippocampus CA1 interneuron oriens alveus cell; AMPA; I Na,t; I A; I K; I h;
proc celldef() {
  topol()
  subsets()
  geom()
  biophys()
  geom_nseg()
}

create soma[9], dend2[6], dend4[22], dend3[2], dend1[20], dend7[10], dend8[11]
create dend6[3], dend5

proc topol() { local i
  connect soma[1](0), soma(0)
  for i = 2, 8 connect soma[i](0), soma[i-2](1)
  connect dend2(0), soma(0)
  for i = 1, 2 connect dend2[i](0), dend2[i-1](1)
  connect dend2[3](0), dend2[1](1)
  for i = 4, 5 connect dend2[i](0), dend2[3](1)
  connect dend4(0), soma(1)
  for i = 1, 2 connect dend4[i](0), dend4(1)
  for i = 3, 4 connect dend4[i](0), dend4[1](1)
  for i = 5, 6 connect dend4[i](0), dend4[2](1)
  for i = 7, 8 connect dend4[i](0), dend4[3](1)
  for i = 9, 10 connect dend4[i](0), dend4[4](1)
  for i = 11, 12 connect dend4[i](0), dend4[6](1)
  for i = 13, 14 connect dend4[i](0), dend4[7](1)
  for i = 15, 16 connect dend4[i](0), dend4[10](1)
  for i = 17, 18 connect dend4[i](0), dend4[11](1)
  for i = 19, 20 connect dend4[i](0), dend4[12](1)
  connect dend4[21](0), dend4[15](1)
  connect dend3(0), dend2(1)
  connect dend3[1](0), dend3(1)
  connect dend1(0), soma[1](1)
  for i = 1, 2 connect dend1[i](0), dend1(1)
  for i = 3, 4 connect dend1[i](0), dend1[i-2](1)
  for i = 5, 8 connect dend1[i](0), dend1[i-3](1)
  for i = 9, 10 connect dend1[i](0), dend1[i-4](1)
  for i = 11, 12 connect dend1[i](0), dend1[i-1](1)
  for i = 13, 14 connect dend1[i](0), dend1[i-2](1)
  for i = 15, 16 connect dend1[i](0), dend1[i-3](1)
  connect dend1[17](0), dend1[13](1)
  for i = 18, 19 connect dend1[i](0), dend1[15](1)
  connect dend7(0), soma[3](1)
  for i = 1, 2 connect dend7[i](0), dend7[i-1](1)
  for i = 3, 4 connect dend7[i](0), dend7[i-2](1)
  for i = 5, 7 connect dend7[i](0), dend7[2](1)
  for i = 8, 9 connect dend7[i](0), dend7[i-5](1)
  connect dend8(0), soma[8](1)
  for i = 1, 4 connect dend8[i](0), dend8(1)
  connect dend8[5](0), dend8[1](1)
  for i = 6, 7 connect dend8[i](0), dend8[5](1)
  connect dend8[8](0), dend8[6](1)
  for i = 9, 10 connect dend8[i](0), dend8[8](1)
  connect dend6(0), soma[8](1)
  for i = 1, 2 connect dend6[i](0), dend6(1)
  connect dend5(0), soma[8](1)
  basic_shape()
}
proc shape3d_1() {
  soma {pt3dclear()
	pt3dadd(-14, -164, 0, 9.22)
	pt3dadd(-11.08, -164, 0, 9.22)
  }
  dend2 {pt3dclear()
	pt3dadd(-14, -164, 0, 1.07)
	pt3dadd(-16.7235, -158.553, 0, 1.07)
  }
  soma[1] {pt3dclear()
	pt3dadd(-14, -164, 0, 8.03)
	pt3dadd(-16.92, -164, 0, 8.03)
  }
  dend4 {pt3dclear()
	pt3dadd(-11.08, -164, 0, 1.38)
	pt3dadd(-11.08, -126.28, 0, 1.38)
  }
  soma[2] {pt3dclear()
	pt3dadd(-11.08, -164, 0, 9.82)
	pt3dadd(-8.16, -164, 0, 9.82)
  }
  dend3 {pt3dclear()
	pt3dadd(-16.7235, -158.553, 0, 0.68)
	pt3dadd(-100.264, -158.553, 0, 0.68)
  }
  dend2[1] {pt3dclear()
	pt3dadd(-16.7235, -158.553, 0, 0.89)
	pt3dadd(-93.3491, -120.24, 0, 0.89)
  }
  dend1 {pt3dclear()
	pt3dadd(-16.92, -164, 0, 1.29)
	pt3dadd(-112.958, -144.792, 0, 1.29)
  }
  soma[3] {pt3dclear()
	pt3dadd(-16.92, -164, 0, 6.78)
	pt3dadd(-19.84, -164, 0, 6.78)
  }
  dend4[1] {pt3dclear()
	pt3dadd(-11.08, -126.28, 0, 1.15)
	pt3dadd(0.279225, -103.562, 0, 1.15)
  }
  dend4[2] {pt3dclear()
	pt3dadd(-11.08, -126.28, 0, 1.07)
	pt3dadd(-11.08, -105.28, 0, 1.07)
  }
  soma[4] {pt3dclear()
	pt3dadd(-8.16, -164, 0, 7.6)
	pt3dadd(-5.24, -164, 0, 7.6)
  }
  dend3[1] {pt3dclear()
	pt3dadd(-100.264, -158.553, 0, 0.64)
	pt3dadd(-161.81, -220.1, 0, 0.64)
  }
  dend2[2] {pt3dclear()
	pt3dadd(-93.3491, -120.24, 0, 0.68)
	pt3dadd(-164.19, -63.5678, 0, 0.68)
  }
  dend2[3] {pt3dclear()
	pt3dadd(-93.3491, -120.24, 0, 0.68)
	pt3dadd(-111.359, -120.24, 0, 0.68)
  }
  dend1[1] {pt3dclear()
	pt3dadd(-112.958, -144.792, 0, 0.86)
	pt3dadd(-120.757, -152.592, 0, 0.86)
  }
  dend1[2] {pt3dclear()
	pt3dadd(-112.958, -144.792, 0, 1.07)
	pt3dadd(-164.51, -127.609, 0, 1.07)
  }
  dend7 {pt3dclear()
	pt3dadd(-19.84, -164, 0, 0.91)
	pt3dadd(-15.0414, -173.597, 0, 0.91)
  }
  soma[5] {pt3dclear()
	pt3dadd(-19.84, -164, 0, 5.87)
	pt3dadd(-22.76, -164, 0, 5.87)
  }
  dend4[3] {pt3dclear()
	pt3dadd(0.279225, -103.562, 0, 0.91)
	pt3dadd(13.3091, -84.0167, 0, 0.91)
  }
  dend4[4] {pt3dclear()
	pt3dadd(0.279225, -103.562, 0, 0.86)
	pt3dadd(0.279225, -71.1015, 0, 0.86)
  }
  dend4[5] {pt3dclear()
	pt3dadd(-11.08, -105.28, 0, 0.86)
	pt3dadd(-24.3204, 7.26383, 0, 0.86)
  }
  dend4[6] {pt3dclear()
	pt3dadd(-11.08, -105.28, 0, 1.07)
	pt3dadd(-75.3074, -23.635, 0, 1.07)
  }
  soma[6] {pt3dclear()
	pt3dadd(-5.24, -164, 0, 4.92)
	pt3dadd(-2.32, -164, 0, 4.92)
  }
  dend2[4] {pt3dclear()
	pt3dadd(-111.359, -120.24, 0, 0.61)
	pt3dadd(-220.009, -120.24, 0, 0.61)
  }
  dend2[5] {pt3dclear()
	pt3dadd(-111.359, -120.24, 0, 0.64)
	pt3dadd(-126.781, -135.662, 0, 0.64)
  }
  dend1[3] {pt3dclear()
	pt3dadd(-120.757, -152.592, 0, 0.86)
	pt3dadd(-131.724, -148.936, 0, 0.86)
  }
  dend1[4] {pt3dclear()
	pt3dadd(-164.51, -127.609, 0, 0.86)
	pt3dadd(-298.417, -144.347, 0, 0.86)
  }
  dend1[5] {pt3dclear()
	pt3dadd(-164.51, -127.609, 0, 0.86)
	pt3dadd(-170.422, -126.426, 0, 0.86)
  }
  dend7[1] {pt3dclear()
	pt3dadd(-15.0414, -173.597, 0, 0.91)
	pt3dadd(-25.6834, -184.239, 0, 0.91)
  }
  soma[7] {pt3dclear()
	pt3dadd(-22.76, -164, 0, 4.15)
	pt3dadd(-25.68, -164, 0, 4.15)
  }
  dend4[7] {pt3dclear()
	pt3dadd(13.3091, -84.0167, 0, 0.88)
	pt3dadd(48.0887, -75.3218, 0, 0.88)
  }
  dend4[8] {pt3dclear()
	pt3dadd(13.3091, -84.0167, 0, 0.86)
	pt3dadd(47.674, -16.0505, 0, 0.86)
  }
  dend4[9] {pt3dclear()
	pt3dadd(0.279225, -71.1015, 0, 0.91)
	pt3dadd(-16.4101, 61.3008, 0, 0.91)
  }
  dend4[10] {pt3dclear()
	pt3dadd(0.279225, -71.1015, 0, 0.86)
	pt3dadd(-7.78179, -63.0405, 0, 0.86)
  }
  dend4[11] {pt3dclear()
	pt3dadd(-75.3074, -23.635, 0, 0.91)
	pt3dadd(-100.596, 13.4543, 0, 0.91)
  }
  dend4[12] {pt3dclear()
	pt3dadd(-75.3074, -23.635, 0, 0.96)
	pt3dadd(-111.346, -6.81688, 0, 0.96)
  }
  soma[8] {pt3dclear()
	pt3dadd(-2.32, -164, 0, 3.24)
	pt3dadd(0.599997, -164, 0, 3.24)
  }
  dend1[6] {pt3dclear()
	pt3dadd(-131.724, -148.936, 0, 0.86)
	pt3dadd(-184.884, -148.936, 0, 0.86)
  }
  dend1[7] {pt3dclear()
	pt3dadd(-298.417, -144.347, 0, 0.86)
	pt3dadd(-298.417, -155.307, 0, 0.86)
  }
  dend1[8] {pt3dclear()
	pt3dadd(-170.422, -126.426, 0, 0.86)
	pt3dadd(-312.032, -126.426, 0, 0.86)
  }
  dend1[9] {pt3dclear()
	pt3dadd(-170.422, -126.426, 0, 0.86)
	pt3dadd(-214.192, -82.6561, 0, 0.86)
  }
  dend7[2] {pt3dclear()
	pt3dadd(-25.6834, -184.239, 0, 0.86)
	pt3dadd(-25.6834, -195.629, 0, 0.86)
  }
  dend7[3] {pt3dclear()
	pt3dadd(-25.6834, -184.239, 0, 0.86)
	pt3dadd(-70.3394, -217.731, 0, 0.86)
  }
  dend4[13] {pt3dclear()
	pt3dadd(48.0887, -75.3218, 0, 0.86)
	pt3dadd(106.58, -63.6235, 0, 0.86)
  }
  dend4[14] {pt3dclear()
	pt3dadd(48.0887, -75.3218, 0, 0.64)
	pt3dadd(67.784, -85.1694, 0, 0.64)
  }
  dend4[15] {pt3dclear()
	pt3dadd(-7.78179, -63.0405, 0, 0.77)
	pt3dadd(-7.78179, -49.2205, 0, 0.77)
  }
  dend4[16] {pt3dclear()
	pt3dadd(-7.78179, -63.0405, 0, 0.77)
	pt3dadd(-31.5911, -51.3376, 0, 0.77)
  }
  dend4[17] {pt3dclear()
	pt3dadd(-100.596, 13.4543, 0, 0.88)
	pt3dadd(-85.3362, 28.7137, 0, 0.88)
  }
  dend4[18] {pt3dclear()
	pt3dadd(-100.596, 13.4543, 0, 0.91)
	pt3dadd(-129.552, 42.4103, 0, 0.91)
  }
  dend4[19] {pt3dclear()
	pt3dadd(-111.346, -6.81688, 0, 0.86)
	pt3dadd(-146.56, 10.7899, 0, 0.86)
  }
  dend4[20] {pt3dclear()
	pt3dadd(-111.346, -6.81688, 0, 0.88)
	pt3dadd(-187.2, 5.8254, 0, 0.88)
  }
  dend8 {pt3dclear()
	pt3dadd(0.599997, -164, 0, 0.91)
	pt3dadd(4.86194, -172.524, 0, 0.91)
  }
  dend6 {pt3dclear()
	pt3dadd(0.599997, -164, 0, 1.09)
	pt3dadd(65.07, -164, 0, 1.09)
  }
  dend5 {pt3dclear()
	pt3dadd(0.599997, -164, 0, 0.91)
	pt3dadd(61.9352, -139.466, 0, 0.91)
  }
  dend1[10] {pt3dclear()
	pt3dadd(-184.884, -148.936, 0, 0.86)
	pt3dadd(-180.154, -153.667, 0, 0.86)
  }
  dend7[4] {pt3dclear()
	pt3dadd(-25.6834, -195.629, 0, 0.86)
	pt3dadd(-12.4872, -200.028, 0, 0.86)
  }
  dend7[5] {pt3dclear()
	pt3dadd(-25.6834, -195.629, 0, 0.77)
	pt3dadd(-53.3343, -278.582, 0, 0.77)
  }
  dend7[6] {pt3dclear()
	pt3dadd(-25.6834, -195.629, 0, 0.86)
	pt3dadd(-25.6834, -218.559, 0, 0.86)
  }
  dend7[7] {pt3dclear()
	pt3dadd(-25.6834, -195.629, 0, 0.86)
	pt3dadd(-38.6013, -234.383, 0, 0.86)
  }
  dend7[8] {pt3dclear()
	pt3dadd(-70.3394, -217.731, 0, 0.77)
	pt3dadd(-173.073, -286.22, 0, 0.77)
  }
  dend4[21] {pt3dclear()
	pt3dadd(-7.78179, -49.2205, 0, 0.77)
	pt3dadd(14.7874, -41.6975, 0, 0.77)
  }
  dend8[1] {pt3dclear()
	pt3dadd(4.86194, -172.524, 0, 0.86)
	pt3dadd(9.89213, -192.645, 0, 0.86)
  }
  dend8[2] {pt3dclear()
	pt3dadd(4.86194, -172.524, 0, 0.77)
	pt3dadd(-83.4101, -238.728, 0, 0.77)
  }
  dend8[3] {pt3dclear()
	pt3dadd(4.86194, -172.524, 0, 0.86)
	pt3dadd(-18.9301, -190.368, 0, 0.86)
  }
  dend8[4] {pt3dclear()
	pt3dadd(4.86194, -172.524, 0, 0.86)
	pt3dadd(31.3759, -179.152, 0, 0.86)
  }
  dend6[1] {pt3dclear()
	pt3dadd(65.07, -164, 0, 0.86)
	pt3dadd(96.8907, -171.955, 0, 0.86)
  }
  dend6[2] {pt3dclear()
	pt3dadd(65.07, -164, 0, 0.86)
	pt3dadd(178.38, -164, 0, 0.86)
  }
  dend1[11] {pt3dclear()
	pt3dadd(-180.154, -153.667, 0, 0.86)
	pt3dadd(-188.108, -158.97, 0, 0.86)
  }
  dend7[9] {pt3dclear()
	pt3dadd(-12.4872, -200.028, 0, 0.86)
	pt3dadd(33.3249, -269.323, 0, 0.86)
  }
  dend8[5] {pt3dclear()
	pt3dadd(9.89213, -192.645, 0, 0.86)
	pt3dadd(20.2865, -223.828, 0, 0.86)
  }
  dend1[12] {pt3dclear()
	pt3dadd(-188.108, -158.97, 0, 0.77)
	pt3dadd(-194.128, -171.009, 0, 0.77)
  }
  dend1[13] {pt3dclear()
	pt3dadd(-188.108, -158.97, 0, 0.77)
	pt3dadd(-291.35, -173.719, 0, 0.77)
  }
  dend8[6] {pt3dclear()
	pt3dadd(20.2865, -223.828, 0, 0.86)
	pt3dadd(49.4123, -252.954, 0, 0.86)
  }
  dend8[7] {pt3dclear()
	pt3dadd(20.2865, -223.828, 0, 0.86)
	pt3dadd(-15.6345, -259.749, 0, 0.86)
  }
  dend1[14] {pt3dclear()
	pt3dadd(-194.128, -171.009, 0, 0.77)
	pt3dadd(-164.947, -188.517, 0, 0.77)
  }
  dend1[15] {pt3dclear()
	pt3dadd(-194.128, -171.009, 0, 0.77)
	pt3dadd(-204.051, -174.316, 0, 0.77)
  }
  dend1[16] {pt3dclear()
	pt3dadd(-291.35, -173.719, 0, 0.77)
	pt3dadd(-305.428, -180.758, 0, 0.77)
  }
  dend1[17] {pt3dclear()
	pt3dadd(-291.35, -173.719, 0, 0.64)
	pt3dadd(-319.27, -164.412, 0, 0.64)
  }
  dend8[8] {pt3dclear()
	pt3dadd(49.4123, -252.954, 0, 0.64)
	pt3dadd(49.4123, -316.314, 0, 0.64)
  }
  dend1[18] {pt3dclear()
	pt3dadd(-204.051, -174.316, 0, 0.77)
	pt3dadd(-212.119, -190.452, 0, 0.77)
  }
  dend1[19] {pt3dclear()
	pt3dadd(-204.051, -174.316, 0, 0.77)
	pt3dadd(-305.796, -194.665, 0, 0.77)
  }
  dend8[9] {pt3dclear()
	pt3dadd(49.4123, -316.314, 0, 0.64)
	pt3dadd(61.0866, -327.988, 0, 0.64)
  }
  dend8[10] {pt3dclear()
	pt3dadd(49.4123, -316.314, 0, 0.64)
	pt3dadd(24.6724, -328.684, 0, 0.64)
  }
}
proc basic_shape() {
  shape3d_1()
}

objref all, Soma, Dendrites, Proximal, Distal
proc subsets() { local i
  objref all, Soma, Dendrites, Proximal, Distal
  all = new SectionList()
    for i=0, 8 soma[i] all.append()
    for i=0, 5 dend2[i] all.append()
    for i=0, 21 dend4[i] all.append()
    for i=0, 1 dend3[i] all.append()
    for i=0, 19 dend1[i] all.append()
    for i=0, 9 dend7[i] all.append()
    for i=0, 10 dend8[i] all.append()
    for i=0, 2 dend6[i] all.append()
    dend5 all.append()

  Soma = new SectionList()
    for i=0, 8 soma[i] Soma.append()

  Dendrites = new SectionList()
    for i=0, 5 dend2[i] Dendrites.append()
    for i=0, 21 dend4[i] Dendrites.append()
    for i=0, 1 dend3[i] Dendrites.append()
    for i=0, 19 dend1[i] Dendrites.append()
    for i=0, 9 dend7[i] Dendrites.append()
    for i=0, 10 dend8[i] Dendrites.append()
    for i=0, 2 dend6[i] Dendrites.append()
    dend5 Dendrites.append()

  Proximal = new SectionList()
    for i=0, 1 dend2[i] Proximal.append()
    for i=0, 4 dend4[i] Proximal.append()
    dend3 Proximal.append()
    dend1 Proximal.append()
    for i=0, 3 dend7[i] Proximal.append()
    dend7[6] Proximal.append()
    for i=0, 5 dend8[i] Proximal.append()
    dend6 Proximal.append()
    dend5 Proximal.append()

  Distal = new SectionList()
    for i=2, 5 dend2[i] Distal.append()
    for i=5, 21 dend4[i] Distal.append()
    dend3[1] Distal.append()
    for i=1, 19 dend1[i] Distal.append()
    for i=4, 5 dend7[i] Distal.append()
    for i=7, 9 dend7[i] Distal.append()
    for i=6, 10 dend8[i] Distal.append()
    for i=1, 2 dend6[i] Distal.append()

}
proc geom() {
}
proc geom_nseg() {
  soma area(.5) // make sure diam reflects 3d points
}
proc biophys() {
  forsec all {
    Ra = 150
    cm = 1.3
  }
  forsec Soma {
    insert IA
      gkAbar_IA = 0.0165
    insert Ih
      gkhbar_Ih = 0.001385
    insert Ksoma
      gksoma_Ksoma = 0.0319
    insert Nasoma
      gnasoma_Nasoma = 0.0107
      gl_Nasoma = 5e-05
      el_Nasoma = -70
  }
  forsec Dendrites {
    insert Kdend
      gkdend_Kdend = 0.023
    insert Nadend
      gnadend_Nadend = 0.0117
      gl_Nadend = 5e-05
      el_Nadend = -70
  }
  forsec Proximal {
    insert IA1
      gkAbar_IA1 = 0.013
  }
}
forall{
	celsius = 24
	insert na_ion ena =90
	insert k_ion ek = -100
	insert h_ion eh = -32.9
}
access soma

celldef()

forall{
	nseg=L/10+1
	}

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