Olfactory Periglomerular Cells: I-h kinetics (Cadetti, Belluzzi 2001)

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Accession:3665
NEURON mod files for the Ih current from the paper: Cadetti L, Belluzzi O. Hyperpolarisation-activated current in glomerular cells of the rat olfactory bulb. Neuroreport 12:3117-20 (2001).
Reference:
1 . Cadetti L, Belluzzi O (2001) Hyperpolarisation-activated current in glomerular cells of the rat olfactory bulb. Neuroreport 12:3117-20 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Channel/Receptor;
Brain Region(s)/Organism:
Cell Type(s):
Channel(s): I h;
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s): Ion Channel Kinetics; Olfaction;
Implementer(s): Migliore, Michele [Michele.Migliore at Yale.edu];
Search NeuronDB for information about:  I h;
load_file("nrngui.hoc")
create a
a	{nseg=1 diam=20 L=20 
	insert hpg eh_hpg=-30 
	insert pas g_pas=1/10000 Ra=150 cm=1}
access a

tstop=300
vlow=-140
vhigh=-50
dt=0.1
celsius=30
color=1

objref gk, b,gt,vc, gs,c, gf,ic
b = new VBox()
c = new VBox()
b.intercept(1)
gk = new Graph(0)
gk.view(vlow,0,vhigh-vlow,1,0,0,100,200)
gk.exec_menu("New Axis")
gk.exec_menu("10% Zoom out")
gk.label(0.1,0.9,"Steady-state; Fig.1b'")
gk.addexpr("linf_hpg",1,2, 2*tstop,0,2)

gt = new Graph(0)
gt.view(vlow,0,vhigh-vlow,600,0,0,100,200)
gt.exec_menu("New Axis")
gt.exec_menu("10% Zoom out")
gt.label(0.1,0.9,"Time constant; Fig.1c'")
gt.addexpr("taul_hpg",2,2, 2*tstop,0,2)

gs = new Graph(0)
gs.view(0,-120,tstop,50,0,0,100,200)
gs.exec_menu("New Axis")
gs.exec_menu("10% Zoom out")
gs.label(0.1,0.9,"Current injection: Fig.1a")
gs.exec_menu("Keep Lines")

xpanel("")
xbutton("run ", "run()")
xvalue("ghbar","ghbar_hpg")
xpanel()
b.intercept(0)
b.map("kinetics from Cadetti and Belluzzi (2001)",100,0,200,600)

c.intercept(1)
gf = new Graph(0)
gf.view(0,-500,1500,500,0,0,100,100)
gf.exec_menu("New Axis")
gf.exec_menu("10% Zoom out")
gf.label(0.3,0.95,"Voltage-clamp (currents in nA) protocol as in Fig.1a'")
gf.exec_menu("Keep Lines")
c.intercept(0)
c.map("activation and deactivation",435,0,500,360)

vc = new SEClamp(0.5)
ic= new IClamp(0.5)

proc run() {
gk.begin()
for (v=vlow; v<vhigh; v=v+1) {
    rate_hpg(v)
    gk.plot(v)
}
gk.flush()
doNotify()

gt.begin()
gt.mark(-70,570,"+",12,3,2)
gt.mark(-80,500,"+",12,3,2)
gt.mark(-90,350,"+",12,3,2)
gt.mark(-100,230,"+",12,3,2)
gt.mark(-110,140,"+",12,3,2)
gt.mark(-120,110,"+",12,3,2)
gt.mark(-130,90,"+",12,3,2)
for (v=vlow; v<vhigh; v=v+1) {
    rate_hpg(v)
    gt.plot(v)
}
gt.flush()
doNotify()

gs.addexpr("a.v(0.5)",color,1, 2*tstop,0,2)

gs.begin()
tstop=300
ic.del=2
ic.dur=tstop
ic.amp=-0.05
t=0
v=-70
finitialize(v)
fcurrent()
e_pas=v+(i_hpg)/g_pas
while (t<tstop) {
    fadvance()
    gs.plot(t)
    }
gs.flush()
doNotify()
ic.amp=0

gf.addexpr("i_hpg*area(0.5)*10",1 ,1, 2*tstop,0,2) // *10=pA, *1e-2=nA
ghbar_hpg=0.0004
tstop=1500
k=-50
while (k>=-140) {
t=0
vc.amp1=-40
vc.dur1=2
vc.amp2=k
vc.dur2=1000
vc.amp3=-40
vc.dur3=500
forall {finitialize(-40)}
fcurrent()
while (t<tstop) {
    fadvance()
    gf.plot(t)
    }
gf.flush()
doNotify()
k=k-10
gf.begin()
}
vc.dur1=0
vc.dur2=0
vc.dur3=0
}



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