Dentate gyrus granule cell: calcium and calcium-dependent conductances (Aradi and Holmes 1999)

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Accession:116740
We have constructed a detailed model of a hippocampal dentate granule (DG) cell that includes nine different channel types. Channel densities and distributions were chosen to reproduce reported physiological responses observed in normal solution and when blockers were applied. The model was used to explore the contribution of each channel type to spiking behavior with particular emphasis on the mechanisms underlying postspike events. ... The model was used to predict changes in channel densities that could lead to epileptogenic burst discharges and to predict the effect of altered buffering capacity on firing behavior. We conclude that the clustered spatial distributions of calcium related channels, the presence of slow delayed rectifier potassium currents in dendrites, and calcium buffering properties, together, might explain the resistance of DG cells to the development of epileptogenic burst discharges.
Reference:
1 . Aradi I, Holmes WR (1999) Role of multiple calcium and calcium-dependent conductances in regulation of hippocampal dentate granule cell excitability. J Comput Neurosci 6:215-35 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism: Dentate gyrus;
Cell Type(s): Dentate gyrus granule GLU cell;
Channel(s): I L high threshold; I N; I T low threshold; I A; I K; I K,Ca; I Calcium; I Potassium;
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s): Bursting; Calcium dynamics;
Implementer(s): Nakhoul, Hani [hnakho at lsuhsc.edu];
Search NeuronDB for information about:  Dentate gyrus granule GLU cell; I L high threshold; I N; I T low threshold; I A; I K; I K,Ca; I Calcium; I Potassium;
{load_file("nrngui.hoc")}
objectvar save_window_, rvp_
objectvar scene_vector_[5]
objectvar ocbox_, ocbox_list_, scene_, scene_list_
{ocbox_list_ = new List()  scene_list_ = new List()}
{pwman_place(0,0,0)}

//Begin ChannelBuild[0] managed KSChan[0]
{
load_file("chanbild.hoc", "ChannelBuild")
}
{ion_register("na", 1)}
{ocbox_ = new ChannelBuild(1)}
{object_push(ocbox_)}
{genprop.set_data("Na", 1, 1, 5, "na")}
{genprop.set_defstr(0, 0)}
tobj = new ChannelBuildKSGate(this)
{gatelist.append(tobj)}
{tobj.begin_restore(3)}
{tobj.set_state("m", 1, 140, 140)}
{tobj.set_trans(0, 0, 0)}
{tobj.transitions.object(0).settype(0, "")}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
1.5
0.2
-45
{tobj.transitions.object(0).set_f(0, 3, tobj1)}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
1.5
-0.2
-17
{tobj.transitions.object(0).set_f(1, 3, tobj1)}
{tobj.end_restore()}
tobj = new ChannelBuildKSGate(this)
{gatelist.append(tobj)}
{tobj.begin_restore(1)}
{tobj.set_state("h", 1, 140, 110)}
{tobj.set_trans(0, 0, 0)}
{tobj.transitions.object(0).settype(0, "")}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
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-0.05
-67
{tobj.transitions.object(0).set_f(0, 2, tobj1)}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
3.33
-0.1
-14.5
{tobj.transitions.object(0).set_f(1, 4, tobj1)}
{tobj.end_restore()}
end_restore()
{genprop.set_single(0)}
{set_alias(0)}
{usetable(0)}
{object_pop()}
{
ocbox_.map("ChannelBuild[0] managed KSChan[0]", 0, 180, 365.4, 702)
}
objref ocbox_
//End ChannelBuild[0] managed KSChan[0]


//Begin ChannelBuild[1] managed KSChan[1]
{
load_file("chanbild.hoc", "ChannelBuild")
}
{ion_register("k", 1)}
{ocbox_ = new ChannelBuild(1)}
{object_push(ocbox_)}
{genprop.set_data("fKDR", 1, 1, 6, "k")}
{genprop.set_defstr(0, 0)}
tobj = new ChannelBuildKSGate(this)
{gatelist.append(tobj)}
{tobj.begin_restore(4)}
{tobj.set_state("n", 1, 140, 140)}
{tobj.set_trans(0, 0, 0)}
{tobj.transitions.object(0).settype(0, "")}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
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0.16667
-23
{tobj.transitions.object(0).set_f(0, 3, tobj1)}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
0.264
-0.025
-48
{tobj.transitions.object(0).set_f(1, 2, tobj1)}
{tobj.end_restore()}
end_restore()
{genprop.set_single(0)}
{set_alias(0)}
{usetable(0)}
{object_pop()}
{
ocbox_.map("ChannelBuild[1] managed KSChan[1]", 300, 180, 365.4, 702)
}
objref ocbox_
//End ChannelBuild[1] managed KSChan[1]


//Begin ChannelBuild[2] managed KSChan[2]
{
load_file("chanbild.hoc", "ChannelBuild")
}
{ion_register("k", 1)}
{ocbox_ = new ChannelBuild(1)}
{object_push(ocbox_)}
{genprop.set_data("sKDR", 1, 1, 6, "k")}
{genprop.set_defstr(0, 0)}
tobj = new ChannelBuildKSGate(this)
{gatelist.append(tobj)}
{tobj.begin_restore(4)}
{tobj.set_state("n", 1, 140, 140)}
{tobj.set_trans(0, 0, 0)}
{tobj.transitions.object(0).settype(0, "")}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
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0.16667
-35
{tobj.transitions.object(0).set_f(0, 3, tobj1)}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
0.1056
-0.025
-60
{tobj.transitions.object(0).set_f(1, 2, tobj1)}
{tobj.end_restore()}
end_restore()
{genprop.set_single(0)}
{set_alias(0)}
{usetable(0)}
{object_pop()}
{
ocbox_.map("ChannelBuild[2] managed KSChan[2]", 594, 180, 342.9, 702)
}
objref ocbox_
//End ChannelBuild[2] managed KSChan[2]


//Begin ChannelBuild[3] managed KSChan[3]
{
load_file("chanbild.hoc", "ChannelBuild")
}
{ion_register("k", 1)}
{ocbox_ = new ChannelBuild(1)}
{object_push(ocbox_)}
{genprop.set_data("KA", 1, 1, 6, "k")}
{genprop.set_defstr(0, 0)}
tobj = new ChannelBuildKSGate(this)
{gatelist.append(tobj)}
{tobj.begin_restore(1)}
{tobj.set_state("k", 1, 70, 140)}
{tobj.set_trans(0, 0, 0)}
{tobj.transitions.object(0).settype(0, "")}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
0.75
0.066667
-25
{tobj.transitions.object(0).set_f(0, 3, tobj1)}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
0.8
-0.125
-15
{tobj.transitions.object(0).set_f(1, 3, tobj1)}
{tobj.end_restore()}
tobj = new ChannelBuildKSGate(this)
{gatelist.append(tobj)}
{tobj.begin_restore(1)}
{tobj.set_state("l", 1, 90, 140)}
{tobj.set_trans(0, 0, 0)}
{tobj.transitions.object(0).settype(0, "")}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
0.00015
-0.066667
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{tobj.transitions.object(0).set_f(0, 2, tobj1)}
{tobj1 = new Vector(3)  for (i=0; i < 3; i += 1) tobj1.x[i] = fscan() }
0.06
-0.083333
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{tobj.transitions.object(0).set_f(1, 4, tobj1)}
{tobj.end_restore()}
end_restore()
{genprop.set_single(0)}
{set_alias(0)}
{usetable(0)}
{object_pop()}
{
ocbox_.map("ChannelBuild[3] managed KSChan[3]", 858, 180, 358.2, 702)
}
objref ocbox_
//End ChannelBuild[3] managed KSChan[3]


//Begin CellBuild[0]
{
load_file("celbild.hoc", "CellBuild")
}
{ocbox_ = new CellBuild(1)}
{object_push(ocbox_)}
{
version(5.7)
continuous = 0
}
{object_push(topol)}
{
first = 0
slist.remove_all()
sname = "distal"
objref tobj
}
{
tobj = new CellBuildSection("soma",0, 0, tobj, 0) slist.append(tobj)
  tobj.position(0,0,15,0) tobj.lx=7.69231 tobj.ly=18.7314 tobj.i3d=0
tobj = new CellBuildSection("axon",0, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(0)
  tobj.position(15,0,30,0) tobj.lx=18.6235 tobj.ly=-11.6329 tobj.i3d=0
tobj = new CellBuildSection("axon",1, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(1)
  tobj.position(30,0,45,0) tobj.lx=41.7004 tobj.ly=-18.9204 tobj.i3d=0
tobj = new CellBuildSection("axon",2, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(2)
  tobj.position(45,0,60,0) tobj.lx=59.919 tobj.ly=-29.8516 tobj.i3d=0
tobj = new CellBuildSection("axon",3, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(3)
  tobj.position(60,0,120,0) tobj.lx=101.215 tobj.ly=-5.56006 tobj.i3d=0
tobj = new CellBuildSection("GCL",0, 0, tobj, 0) slist.append(tobj)
tobj.parent=slist.object(0)
  tobj.position(0,0,-30,30) tobj.lx=-5.66801 tobj.ly=23.5897 tobj.i3d=0
tobj = new CellBuildSection("GCL",1, 0, tobj, 0) slist.append(tobj)
tobj.parent=slist.object(0)
  tobj.position(0,0,-30,-30) tobj.lx=-3.23887 tobj.ly=-22.5641 tobj.i3d=0
tobj = new CellBuildSection("prox",0, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(5)
  tobj.position(-30,30,-45,30) tobj.lx=-26.3158 tobj.ly=49.0958 tobj.i3d=0
tobj = new CellBuildSection("prox",1, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(6)
  tobj.position(-30,-30,-45,-30) tobj.lx=-11.7409 tobj.ly=-46.8556 tobj.i3d=0
tobj = new CellBuildSection("middle",0, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(7)
  tobj.position(-45,30,-60,30) tobj.lx=-65.1822 tobj.ly=49.0958 tobj.i3d=0
tobj = new CellBuildSection("middle",1, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(8)
  tobj.position(-45,-30,-60,-30) tobj.lx=-61.5385 tobj.ly=-46.8556 tobj.i3d=0
tobj = new CellBuildSection("distal",0, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(9)
  tobj.position(-60,30,-120,30) tobj.lx=-107.692 tobj.ly=49.0958 tobj.i3d=0
tobj = new CellBuildSection("distal",1, 0, tobj, 1) slist.append(tobj)
tobj.parent=slist.object(10)
  tobj.position(-60,-30,-120,-30) tobj.lx=-114.98 tobj.ly=-46.8556 tobj.i3d=0
all_init()
}
for i=0, slist.count-1 {slist.object(i).rdses()}
{object_pop()}
{
}
{object_push(subsets)}
{first = 0}
{ tobj = snlist.object(0)}
{tobj = new SNList("dendrites") snlist.append(tobj)}
for i=0,7 tobj.add(bild.topol.slist.object(fscan()))
5
6
7
8
9
10
11
12
{tobj = new SNList("GCLs") snlist.append(tobj)}
for i=0,1 tobj.add(bild.topol.slist.object(fscan()))
5
6
{tobj = new SNList("proxs") snlist.append(tobj)}
for i=0,1 tobj.add(bild.topol.slist.object(fscan()))
7
8
{tobj = new SNList("middles") snlist.append(tobj)}
for i=0,1 tobj.add(bild.topol.slist.object(fscan()))
9
10
{tobj = new SNList("distals") snlist.append(tobj)}
for i=0,1 tobj.add(bild.topol.slist.object(fscan()))
11
12
{tobj = new SNList("allaxon") snlist.append(tobj)}
for i=0,3 tobj.add(bild.topol.slist.object(fscan()))
1
2
3
4
{consist()}
{object_pop()}
{
}
{object_push(geom)}
{
first = 0
tobj = new GeoSpec(2)
tobj.value = 150
bild.subsets.snlist.object(1).geo.append(tobj)
tobj = new GeoSpec(3)
tobj.value = 3
bild.subsets.snlist.object(1).geo.append(tobj)
tobj = new GeoSpec(6)
tobj.value = 4
bild.subsets.snlist.object(1).geo.append(tobj)
tobj = new GeoSpec(6)
tobj.value = 2
bild.subsets.snlist.object(2).geo.append(tobj)
tobj = new GeoSpec(2)
tobj.value = 50
bild.subsets.snlist.object(2).geo.append(tobj)
tobj = new GeoSpec(1, bild.subsets.snlist.object(6))
for i=0, 3 {tobj.snlist.list.object(i).ldiaminfo.x[1] = fscan()}}
0.9
0.7
0.5
0.4
{
bild.subsets.snlist.object(6).geo.append(tobj)
tobj = new GeoSpec(2)
tobj.value = 50
bild.subsets.snlist.object(6).geo.append(tobj)
tobj = new GeoSpec(6)
tobj.value = 1
bild.subsets.snlist.object(6).geo.append(tobj)
tobj = new GeoSpec(6)
tobj.value = 2
bild.topol.slist.object(0).geo.append(tobj)
tobj = new GeoSpec(2)
tobj.value = 16.8
bild.topol.slist.object(0).geo.append(tobj)
tobj = new GeoSpec(3)
tobj.value = 16.8
bild.topol.slist.object(0).geo.append(tobj)
tobj = new GeoSpec(2)
tobj.value = 1400
bild.topol.slist.object(4).geo.append(tobj)
tobj = new GeoSpec(6)
tobj.value = 28
bild.topol.slist.object(4).geo.append(tobj)
set_default()
}
{object_pop()}
{
}
{object_push(memb)}
{first=0}
{
tobj = new FakeMechStan(0)
tobj.value = 210
tobj.set_default()
tobj = new MStanWrap(tobj, 0)
bild.subsets.snlist.object(0).ml.append(tobj)
}
{
tobj = new FakeMechStan(1)
tobj.value = 1
tobj.set_default()
tobj = new MStanWrap(tobj, 0)
bild.subsets.snlist.object(0).ml.append(tobj)
}
{
tobj = new MechanismStandard("pas")
tobj.set("g_pas", 2.5e-05, 0)
tobj.set("e_pas", -70, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(0).ml.append(tobj)
}
{
tobj = new FakeMechStan(1)
tobj.value = 1.6
tobj.set_default()
tobj = new MStanWrap(tobj, 0)
bild.subsets.snlist.object(1).ml.append(tobj)
}
{
tobj = new MechanismStandard("pas")
tobj.set("g_pas", 4e-05, 0)
tobj.set("e_pas", -70, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(1).ml.append(tobj)
}
{
tobj = new FakeMechStan(1)
tobj.value = 1
tobj.set_default()
tobj = new MStanWrap(tobj, 0)
bild.subsets.snlist.object(2).ml.append(tobj)
}
{
tobj = new MechanismStandard("pas")
tobj.set("g_pas", 2.5e-05, 0)
tobj.set("e_pas", -70, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(2).ml.append(tobj)
}
{
tobj = new MechanismStandard("Ca")
tobj.set("gtcabar_Ca", 0.0003, 0)
tobj.set("gncabar_Ca", 0.003, 0)
tobj.set("glcabar_Ca", 0.015, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(2).ml.append(tobj)
}
{
tobj = new MechanismStandard("CadepK")
tobj.set("gbkbar_CadepK", 0.0003, 0)
tobj.set("gskbar_CadepK", 0.0002, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(2).ml.append(tobj)
}
{
tobj = new MechanismStandard("Na")
tobj.set("gmax_Na", 0.018, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(2).ml.append(tobj)
}
{
tobj = new MechanismStandard("fKDR")
tobj.set("gmax_fKDR", 0.004, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(2).ml.append(tobj)
}
{
tobj = new MechanismStandard("sKDR")
tobj.set("gmax_sKDR", 0.003, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(2).ml.append(tobj)
}
{
tobj = new MechanismStandard("Na")
tobj.set("gmax_Na", 0.013, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(3).ml.append(tobj)
}
{
tobj = new MechanismStandard("fKDR")
tobj.set("gmax_fKDR", 0.004, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(3).ml.append(tobj)
}
{
tobj = new MechanismStandard("sKDR")
tobj.set("gmax_sKDR", 0.003, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(3).ml.append(tobj)
}
{
tobj = new MechanismStandard("Ca")
tobj.set("gtcabar_Ca", 0.001, 0)
tobj.set("gncabar_Ca", 0.001, 0)
tobj.set("glcabar_Ca", 0.015, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(3).ml.append(tobj)
}
{
tobj = new MechanismStandard("CadepK")
tobj.set("gbkbar_CadepK", 0.0005, 0)
tobj.set("gskbar_CadepK", 0.0001, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(3).ml.append(tobj)
}
{
tobj = new MechanismStandard("Ca")
tobj.set("gtcabar_Ca", 0.002, 0)
tobj.set("gncabar_Ca", 0.001, 0)
tobj.set("glcabar_Ca", 0.001, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(4).ml.append(tobj)
}
{
tobj = new MechanismStandard("CadepK")
tobj.set("gbkbar_CadepK", 0.0012, 0)
tobj.set("gskbar_CadepK", 0, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(4).ml.append(tobj)
}
{
tobj = new MechanismStandard("Na")
tobj.set("gmax_Na", 0.008, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(4).ml.append(tobj)
}
{
tobj = new MechanismStandard("fKDR")
tobj.set("gmax_fKDR", 0.001, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(4).ml.append(tobj)
}
{
tobj = new MechanismStandard("sKDR")
tobj.set("gmax_sKDR", 0.003, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(4).ml.append(tobj)
}
{
tobj = new MechanismStandard("Ca")
tobj.set("gtcabar_Ca", 0.002, 0)
tobj.set("gncabar_Ca", 0.001, 0)
tobj.set("glcabar_Ca", 0, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(5).ml.append(tobj)
}
{
tobj = new MechanismStandard("CadepK")
tobj.set("gbkbar_CadepK", 0.0012, 0)
tobj.set("gskbar_CadepK", 0, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(5).ml.append(tobj)
}
{
tobj = new MechanismStandard("fKDR")
tobj.set("gmax_fKDR", 0.001, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(5).ml.append(tobj)
}
{
tobj = new MechanismStandard("sKDR")
tobj.set("gmax_sKDR", 0.004, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(5).ml.append(tobj)
}
{
tobj = new MechanismStandard("Na")
tobj.set("gmax_Na", 0.21, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(6).ml.append(tobj)
}
{
tobj = new MechanismStandard("fKDR")
tobj.set("gmax_fKDR", 0.028, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(6).ml.append(tobj)
}
{
tobj = new MechanismStandard("KA")
tobj.set("gmax_KA", 0.004, 0)
tobj = new MStanWrap(tobj, 1)
bild.subsets.snlist.object(6).ml.append(tobj)
}
{
tobj = new MechanismStandard("Ca")
tobj.set("gtcabar_Ca", 0.00015, 0)
tobj.set("gncabar_Ca", 0.002, 0)
tobj.set("glcabar_Ca", 0.01, 0)
tobj = new MStanWrap(tobj, 1)
bild.topol.slist.object(0).ml.append(tobj)
}
{
tobj = new MechanismStandard("CadepK")
tobj.set("gbkbar_CadepK", 0.0003, 0)
tobj.set("gskbar_CadepK", 0.0005, 0)
tobj = new MStanWrap(tobj, 1)
bild.topol.slist.object(0).ml.append(tobj)
}
{
tobj = new MechanismStandard("Na")
tobj.set("gmax_Na", 0.12, 0)
tobj = new MStanWrap(tobj, 1)
bild.topol.slist.object(0).ml.append(tobj)
}
{
tobj = new MechanismStandard("fKDR")
tobj.set("gmax_fKDR", 0.016, 0)
tobj = new MStanWrap(tobj, 1)
bild.topol.slist.object(0).ml.append(tobj)
}
{
tobj = new MechanismStandard("sKDR")
tobj.set("gmax_sKDR", 0.003, 0)
tobj = new MStanWrap(tobj, 1)
bild.topol.slist.object(0).ml.append(tobj)
}
{
tobj = new MechanismStandard("KA")
tobj.set("gmax_KA", 0.012, 0)
tobj = new MStanWrap(tobj, 1)
bild.topol.slist.object(0).ml.append(tobj)
}
{object_pop()}
{
}
{object_push(manage)}
{
first = 0
classname = "Cell"
etop=1 esub=1 egeom=1 emem=1
itop=1 isub=0 igeom=0 imem=0
bild.topol.names_off = 0
bild.topol.circles_off = 0
output_index = 0  output_x = 1
thresh = 10
}
{object_pop()}
{
cexport()
}
{object_pop()}
{
save_window_=ocbox_.gtopol
save_window_.size(-200,200,-150,150)
scene_vector_[2] = save_window_
ocbox_.gtopol = save_window_
save_window_.save_name("ocbox_.gtopol")
}
{
ocbox_.map("CellBuild[0]", 49, 66, 723.6, 477.9)
}
objref ocbox_
//End CellBuild[0]

objectvar scene_vector_[1]
{doNotify()}

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