Cerebellar purkinje cell: K and Ca channels regulate APs (Miyasho et al 2001)

 Download zip file   Auto-launch 
Help downloading and running models
Accession:17664
We adopted De Schutter and Bower's model as the starting point, then modified the descriptions of several ion channels, such as the P-type Ca channel and the delayed rectifier K channel, and added class-E Ca channels and D-type K channels to the model. Our new model reproduces most of our experimental results and supports the conclusions of our experimental study that class-E Ca channels and D-type K channels are present and functioning in the dendrites of Purkinje neurons.
Reference:
1 . Miyasho T, Takagi H, Suzuki H, Watanabe S, Inoue M, Kudo Y, Miyakawa H (2001) Low-threshold potassium channels and a low-threshold calcium channel regulate Ca2+ spike firing in the dendrites of cerebellar Purkinje neurons: a modeling study. Brain Res 891:106-15 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism:
Cell Type(s): Cerebellum Purkinje GABA cell;
Channel(s): I Na,p; I Na,t; I T low threshold; I A; I K; I M; I K,Ca; I Sodium; I Calcium; I Potassium; I A, slow; I_KD;
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s): Action Potential Initiation; Activity Patterns; Dendritic Action Potentials; Bursting; Active Dendrites; Influence of Dendritic Geometry; Detailed Neuronal Models; Action Potentials; Calcium dynamics;
Implementer(s): Miyakawa, H [Miyakawa at ls.toyaku.ac.jp];
Search NeuronDB for information about:  Cerebellum Purkinje GABA cell; I Na,p; I Na,t; I T low threshold; I A; I K; I M; I K,Ca; I Sodium; I Calcium; I Potassium; I A, slow; I_KD;
/
prknj
readme
CaE.mod *
CalciumP.mod *
CaP.mod *
CaP2.mod *
CaT.mod *
K2.mod *
K22.mod *
K23.mod *
KA.mod *
KC.mod *
KC2.mod *
KC3.mod *
KD.mod *
Kdr.mod *
Kh.mod *
Khh.mod *
KM.mod *
Leak.mod *
NaF.mod *
NaP.mod *
mosinit.hoc
purkinje.hoc
purkinje.ses
                            
TITLE decay of submembrane calcium concentration
: Internal calcium concentration due to calcium currents and pump.
: Differential equations.
:
: This file contains two mechanisms:
:
: 1. Simple model of ATPase pump with 3 kinetic constants (Destexhe 1992)
:
:      Cai + P <-> CaP -> Cao + P  (k1,k2,k3)
:
: A Michaelis-Menten approximation is assumed, which reduces the complexity
: of the system to 2 parameters:
:    kt = <tot enzyme concentration> * k3 -> TIME CONSTANT OF THE PUMP
:    kd = k2/k1 (dissociation constant)  -> EQUILIBRIUM CALCIUM VALE
: The values of these parameters are chosen assuming a high affinity of
: the pump to calcium and a low transport capacity (cfr. Blaustein,
: TINS, 11: 438, 1988, and references therein).
:
: For further information about this this mechanism, see Destexhe,A.
: Babloysntz,A. and Sejnowski,TJ. Ionic mechanisms for intrinsic slow
: oscillations in thalamic relay neurons. Biophys.J.65:1538-1552,1933.
:
:
: 2. Simple first-order decay or buffering:
:
:      Cai + B <->...
:
: which can be ritten as:
:
:      dCai/dt = (cainf-Cai) / taur
:
: where cainf is the equilibrium intracellular calcium value (usually
: inthe range of 200-300 nM) and tsur is the time constant of calcium
: removal. The dynamics of submembranal calcium is usually thought to
: be relativly fast, inthe 1-10 millisecond range (see Balaustein,
: TINS, 11:438,1988).
:
: All variables are range variables
:
: Written by Alain Destexhe, Salk Institute,Nov 12,1992 
: 

INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)}

NEURON {
	SUFFIX cad
	USEION ca READ ica,cai WRITE cai
	RANGE depth,kt,kd,cainf,taur
}

UNITS {
	(molar) = (1/liter)      :moles do not appear in units
	(mM)	= (millimolar)
	(um)	= (micron)
	(mA)	= (milliamp)
	(msM)   = (ms mM)
}

CONSTANT{
	FARADAY = 96489 (coul)   : moles do not appear in units
}

PARAMETER {
	depth = .1	(um)     : depth of shell
	taur  = 1e10    (ms)     : remove first-order decay
	cainf = 2.4e-4	(mM)
	kt    = 1e-4	(mM/ms)
	kd    = 1e-4	(mM)
}

STATE {
	cai   (mM)
}

INITIAL {
	cai = kd
}

ASSIGNED{
	ica		(mA/cm2)
	drive_channel   (mM/ms)
	drive_pump	(mM/ms)
}

BREAKPOINT{
	SOLVE state METHOD derivimplicit
}

DERIVATIVE state {

	drive_channel = -(10000)*ica/(2*FARADAY*depth)

	if(drive_channel <= 0.) {drive_channel = 0.}:cannot pump inward

	drive_pump = -kt*cai/(cai+kd)  :Michaelis-Menten

	cai' =drive_channel+drive_pump+(cainf-cai)/taur
}

Loading data, please wait...