" ... With the aid of patch-clamp technology and simulation modeling,
the effects of (Rotenone) Rot on membrane ion currents present in
mHippoE-14 cells were investigated. Results: Addition of Rot produced
an inhibitory action on the peak amplitude of INa ...; however,
neither activation nor inactivation kinetics of INa was changed during
cell exposure to this compound. Addition of Rot produced little or no
modifications in the steady-state inactivation curve of INa. Rot
increased the amplitude of Ca2+-activated Cl- current in response to
membrane depolarization ... . Moreover, when these cells were exposed
to 10 µM Rot, a specific population of ATP-sensitive K+ channels
... was measured, despite its inability to alter single-channel
conductance. Under current clamp condition, the frequency of miniature
end-plate potentials in mHippoE-14 cells was significantly raised in
the presence of Rot (10 µM) with no changes in their amplitude and
time course of rise and decay. In simulated model of hippocampal
neurons incorporated with chemical autaptic connection, increased
autaptic strength to mimic the action of Rot was noted to change the
bursting pattern with emergence of subthreshold
potentials. Conclusions: The Rot effects presented herein might exert
a significant action on functional activities of hippocampal neurons
occurring in vivo. "
Reference:
1 .
Huang CW, Lin KM, Hung TY, Chuang YC, Wu SN (2018) Multiple Actions of Rotenone, an Inhibitor of Mitochondrial Respiratory Chain, on Ionic Currents and Miniature End-Plate Potential in Mouse Hippocampal (mHippoE-14) Neurons. Cell Physiol Biochem 47:330-343 [PubMed]
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