strdef cmd
objref vbox1, vbox2, vbox3, vbox4
objref gv1, gi2, gi3
objref volt_cl
objref peak_vec
objref v_vec
objref ms
create soma
soma {
diam = 21.851
L = 21.851
nseg = 1
cm = 1
Ra = 60
insert CaR
cai0_ca_ion = 0.000001
cao0_ca_ion = 4
celsius = 22
volt_cl = new VClamp_plus(.5)
}
vbox4 = new VBox()
vbox4.intercept(1)
ms = new MechanismStandard("CaR")
ms.action("change_CaR()")
ms.in()
ms.panel()
vbox4.intercept(0)
vbox4.map("CaR Control", 327, 783, -1, -1)
proc change_CaR() {
ms.out()
}
y_min = -0.2
displ = 1
incr = 10
dens = 0
cl_st = -120
cl_end = 10
peak_vec = new Vector()
v_vec = new Vector()
soma volt_cl=new VClamp_plus(.5)
proc Clamp() {
peak_curr = 0
volt_cl.amp[1] = $1
finitialize(hold_pot)
while (t<tstop) {
dens=soma.ica(.5)
if (displ==1) {
gv1.line(t, soma.v(.5))
gv1.flush()
gi2.line(t, dens)
gi2.flush()
}
if (t>(dur_st_cond+10)) {
if (abs(dens)>peak_curr) {
peak_curr=abs(dens)
}
}
fadvance()
}
peak_vec.append(peak_curr)
v_vec.append($1)
print peak_curr, $1
doEvents()
}
proc erase() {
// gv1.erase(0)
// gi2.erase(0)
gv1.size(0, tstop, cl_st, cl_end)
gi2.size(volt_cl.dur[0]+dur_st_cond, volt_cl.dur[0]+dur_st_cond+dur_st_test+volt_cl.dur[3], y_min, 0.1)
gv1.beginline()
gi2.beginline(2,1)
}
proc start() {
peak_vec.resize(0)
v_vec.resize(0)
Tstop()
for (i=cl_st; i<=cl_end; i=i+incr) {
Clamp(i)
erase()
}
peak_norm = peak_vec.max()
for i=0, peak_vec.size()-1 {
peak_vec.x[i]=peak_vec.x[i]/peak_norm
}
gi3.erase()
gi3.size(cl_st-10, cl_end+10, 0, 1)
gi3.begin()
peak_vec.line(gi3,v_vec,1,1)
for i=0, peak_vec.size()-1 {
gi3.flush()
doNotify()
}
}
proc Tstop() {
volt_cl.dur[0] = 5
volt_cl.amp[0] = hold_pot
volt_cl.dur[1] = dur_st_cond
volt_cl.dur[2] = dur_st_test
volt_cl.amp[2] = amp_st_test
volt_cl.dur[3] = 5
volt_cl.amp[3] = hold_pot
tstop = 0
for i=0, 3 {
tstop = tstop + volt_cl.dur[i]
}
}
access soma
execute(cmd)
{
xpanel("Run Control", 0)
hold_pot = -80
v_init = hold_pot
xvalue("Init","v_init", 1,"stdinit()", 1, 1 )
xbutton("Init & Run","run()")
xbutton("Stop","stoprun=1")
runStopAt = 5
xvalue("Continue til","runStopAt", 1,"{continuerun(runStopAt) stoprun=1}", 1, 1 )
runStopIn = 1
xvalue("Continue for","runStopIn", 1,"{continuerun(t + runStopIn) stoprun=1}", 1, 1 )
xbutton("Single Step","steprun()")
t = 140
xvalue("t","t", 2 )
tstop = 140
xvalue("Tstop","tstop", 1,"tstop_changed()", 0, 1 )
dt = 0.25
xvalue("dt","dt", 1,"setdt()", 0, 1 )
steps_per_ms = 40
xvalue("Points plotted/ms","steps_per_ms", 1,"setdt()", 0, 1 )
screen_update_invl = 0.05
xvalue("Scrn update invl","screen_update_invl", 1,"", 0, 1 )
realtime = 0.00999999
xvalue("Real Time","realtime", 0,"", 0, 1 )
xpanel(2,102)
}
{
xpanel("Clamp Control", 0)
xbutton("Start","start()")
hold_pot=v_init
volt_cl.amp[0]=hold_pot
xvalue("Holding voltage", "volt_cl.amp[0]")
volt_cl.dur[0]=5
xvalue("Pre-conditioning duration", "volt_cl.dur[0]", 1, "Tstop()")
dur_st_cond = 2000
xvalue("Conditioning duration", "dur_st_cond", 1, "Tstop()")
amp_st_test = 10
xvalue("Test pulse voltage", "amp_st_test")
dur_st_test = 25
xvalue("Test pulse duration", "dur_st_test", 1, "Tstop()")
volt_cl.amp[3]=hold_pot
xvalue("Post-test voltage", "volt_cl.amp[3]")
volt_cl.dur[3]=5
xvalue("Post-test duration", "volt_cl.dur[3]", 1, "Tstop()")
xvalue("Start voltage", "cl_st")
xvalue("End voltage", "cl_end")
xvalue("Increment", "incr")
xstatebutton("Display", &displ)
xpanel(2,537)
}
vbox1=new VBox()
vbox1.intercept(1)
gv1=new Graph()
gv1.size(0, tstop, cl_st, cl_end)
vbox1.intercept(0)
vbox1.map("Voltage protocol", 330, 56, 531, 136.9)
vbox2=new VBox()
vbox2.intercept(1)
gi2=new Graph()
gi2.size(volt_cl.dur[0]+dur_st_cond, volt_cl.dur[0]+dur_st_cond+dur_st_test+volt_cl.dur[3], y_min, 0.1)
vbox2.intercept(0)
vbox2.map("PPI currents", 330, 321, 531, 287)
vbox3=new VBox()
vbox3.intercept(1)
gi3=new Graph()
gi3.size(cl_st-10, cl_end+10, 0, 1)
vbox3.intercept(0)
vbox3.map("PPI relationship", 1057, 321, 329.4, 287)
