Cl- homeostasis in immature hippocampal CA3 neurons (Kolbaev et al 2020)

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Accession:266811
Model used for the revision of the manuscript. Insertion of a passive Cl- flux and an active Cl-accumulation. Parameters adapted to match the properties of [Cl-]i determined in immature rat CA3 neurons in-vitro.
Reference:
1 . Kolbaev SN, Mohapatra N, Chen R, Lombardi A, Staiger JF, Luhmann HJ, Jedlicka P, Kilb W (2020) NKCC-1 mediated Cl- uptake in immature CA3 pyramidal neurons is sufficient to compensate phasic GABAergic inputs. Sci Rep 10:18399 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell; Synapse;
Brain Region(s)/Organism: Hippocampus;
Cell Type(s):
Channel(s): NKCC1;
Gap Junctions:
Receptor(s): GabaA;
Gene(s):
Transmitter(s): Gaba;
Simulation Environment: NEURON;
Model Concept(s): Synaptic Plasticity; Homeostasis;
Implementer(s): Jedlicka, Peter [jedlicka at em.uni-frankfurt.de]; Kilb, Werner [wkilb at uni-mainz.de];
Search NeuronDB for information about:  GabaA; NKCC1; Gaba;
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Models_Kolbaev et al Scientific Reports Revision
Readme.txt
asin.mod *
cldif_CA3_NKCC1_HCO3.mod *
gabaA_Cl_HCO3.mod *
tonic.mod *
vecevent.mod *
Add_tonic_Cl-current.hoc
anyl.m
Block-Tonic-Cl-current.ses
Cell_1_SciRep_ShrinkCorr.hoc
Determine_cl-Flux_w-o_NKCC1_rig.ses
Determine_R_input_rig.ses
Determine_tau_NKCC1_rig.ses
Display_Phasic-Cl-current.ses
Display_Phasic-Cl-current_for_Charge_Transfer.ses
Display_Phasic-Cl-current_forFreq.ses
Phasic_GABA_activity_Div_Freq.hoc
Phasic_GABA_activity_Div_gGABA.hoc
Phasic_GABA_activity_only_soma_Backregul.hoc
Phasic_GABA_activity_only_soma_Div_Freq.hoc
Phasic_GABA_activity_only_soma_Div_gGABA.hoc
Phasic_GABA_activity_only_soma_for_Charge_Transfer.hoc
Phasic_GABA_activity_only_soma_PlaceSynapsesForFigure.hoc
start_Add_Tonic_Cl-currents.hoc
start_Block_Tonic_Cl-currents.hoc
start_Phasic_Cl-currents.hoc
start_Phasic_Cl-currents_for _Change-transfer.hoc
start_Phasic_GABA_activity_Div_Freq.hoc
start_Phasic_GABA_activity_only_soma_Backregul.hoc
start_Phasic_GABA_activity_only_soma_Div_Freq.hoc
start_Phasic_GABA_activity_only_soma_Div_gGABA.hoc
Switch_off_tonic_Cl-current.hoc
                            
:  Vector stream of events

NEURON {
	THREADSAFE
	ARTIFICIAL_CELL VecStim
	POINTER ptr
}

ASSIGNED {
	index
	etime (ms)
	ptr
}


INITIAL {
	index = 0
	element()
	if (index > 0) {
		net_send(etime - t, 1)
	}
}

NET_RECEIVE (w) {
	if (flag == 1) {
		net_event(t)
		element()
		if (index > 0) {
			net_send(etime - t, 1)
		}
	}
}

DESTRUCTOR {
VERBATIM
	void* vv = (void*)(_p_ptr);
        if (vv) {
		hoc_obj_unref(*vector_pobj(vv));
	}
ENDVERBATIM
}

PROCEDURE element() {
VERBATIM
  { void* vv; int i, size; double* px;
	i = (int)index;
	if (i >= 0) {
		vv = (void*)(_p_ptr);
		if (vv) {
			size = vector_capacity(vv);
			px = vector_vec(vv);
			if (i < size) {
				etime = px[i];
				index += 1.;
			}else{
				index = -1.;
			}
		}else{
			index = -1.;
		}
	}
  }
ENDVERBATIM
}

PROCEDURE play() {
VERBATIM
	void** pv;
	void* ptmp = NULL;
	if (ifarg(1)) {
		ptmp = vector_arg(1);
		hoc_obj_ref(*vector_pobj(ptmp));
	}
	pv = (void**)(&_p_ptr);
	if (*pv) {
		hoc_obj_unref(*vector_pobj(*pv));
	}
	*pv = ptmp;
ENDVERBATIM
}

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