Dendritic Impedance in Neocortical L5 PT neurons (Kelley et al. 2021)

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Accession:266851
We simulated chirp current stimulation in the apical dendrites of 5 biophysically-detailed multi-compartment models of neocortical pyramidal tract neurons and found that a combination of HCN channels and TASK-like channels produced the best fit to experimental measurements of dendritic impedance. We then explored how HCN and TASK-like channels can shape the dendritic impedance as well as the voltage response to synaptic currents.
Reference:
1 . Kelley C, Dura-Bernal S, Neymotin SA, Antic SD, Carnevale NT, Migliore M, Lytton WW (2021) Effects of Ih and TASK-like shunting current on dendritic impedance in layer 5 pyramidal-tract neurons. J Neurophysiology 125:1501-1516 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism:
Cell Type(s): Neocortex L5/6 pyramidal GLU cell; Neocortex M1 L5B pyramidal pyramidal tract GLU cell;
Channel(s): I h; TASK channel;
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: NEURON; Python; NetPyNE;
Model Concept(s): Impedance;
Implementer(s): Kelley, Craig;
Search NeuronDB for information about:  Neocortex L5/6 pyramidal GLU cell; Neocortex M1 L5B pyramidal pyramidal tract GLU cell; I h; TASK channel;
# Load in the goods
import numpy as np
import sys
from scipy.io import savemat
from math import nan

# parse cmd line inputs, load PT cell template
## load cell
from getCells import KoleCell
pt_cell = KoleCell()

## specify stimulated section and soma segment
section = sys.argv[-1]
if section.split('.')[1][:4] == 'apic':
    stim_sec = pt_cell.apic[int(section.split('.')[1].split('[')[1].split(']')[0])]
else:
    stim_sec = pt_cell.dend[int(section.split('.')[1].split('[')[1].split(']')[0])]
soma_seg = pt_cell.soma(0.5)

## specify factors to change gbar ih/im by, ih comes first
if sys.argv[-3][0] == 'm':
    ih_factor = -0.15
else:
    ih_factor = float(sys.argv[-3])
if sys.argv[-2][0] == 'm':
    im_factor = -0.15
else:
    im_factor = float(sys.argv[-2])

# changes to Im/Ih
## determine original values
orig_km = []
orig_ih = []
for seg in stim_sec.allseg():
    try:
        orig_km.append(seg.Km.gbar)
    except:
        orig_km.append(0)
    try:
        orig_ih.append(seg.ih.ghdbar)
    except:
        orig_ih.append(0)
## change Ih/Im
count = 0
for seg in stim_sec.allseg():
    try:
        seg.Km.gbar = orig_km[count] + im_factor * orig_km[count]
    except:
        pass
    try:
        seg.ih.ghdbar = orig_ih[count] + ih_factor * orig_ih[count]
    except:
        pass
    count = count + 1

# define current stimulus
from chirpUtils import applyChirp
from chirpUtils import getChirp
amp = 0.0025
f0, f1, t0, Fs, delay = 0.5, 50, 50, 1000, 12
I, t = getChirp(f0, f1, t0, amp, Fs, delay)

# define output variables
ZinResAmp = []
ZinResFreq = []
ZcResAmp = []
ZcResFreq = []
dist = []
QfactorIn = []
QfactorTrans = []
fVarIn = []
fVarTrans = []
ZinPeakPhaseFreq = []
ZinLeadPhaseBW = []
ZinLeadPhaseMinFreq = []
ZinSynchFreq = []
ZinLeadPhaseBool = []
ZcPeakPhaseFreq = []
ZcLeadPhaseBW = []
ZcLeadPhaseMinFreq = []
ZcSynchFreq = []
ZcLeadPhaseBool = []

# main loop
nseg = stim_sec.nseg
if nseg == 1:
    loc = 0.5
    out = applyChirp(I, t, stim_sec(loc), soma_seg, t0, delay, Fs, f1)
    ZinResAmp.append(out['ZinResAmp'])
    ZinResFreq.append(out['ZinResFreq'])
    ZcResAmp.append(out['ZcResAmp'])
    ZcResFreq.append(out['ZcResFreq'])
    dist.append(out['dist'])
    QfactorIn.append(out['QfactorIn'])
    QfactorTrans.append(out['QfactorTrans'])
    fVarIn.append(out['fVarIn'])
    fVarTrans.append(out['fVarTrans'])

    freqs = out['Freq'][np.argwhere(out['ZinPhase'] > 0)]
    if len(freqs) > 0:
        ZinPeakPhaseFreq.append(out['Freq'][np.argwhere(out['ZinPhase'] == np.max(out['ZinPhase']))])
        ZinLeadPhaseBW.append(freqs[-1] - freqs[0])
        ZinLeadPhaseMinFreq.append(freqs[0])
        ZinSynchFreq.append(freqs[-1])
        ZinLeadPhaseBool.append(out['ZinPhase'] > 0)
    else:
        ZinPeakPhaseFreq.append(nan)
        ZinLeadPhaseBW.append(0)
        ZinLeadPhaseMinFreq.append(nan)
        ZinSynchFreq.append(nan)
        ZinLeadPhaseBool.append(out['ZinPhase'] > 0)

    freqs = out['Freq'][np.argwhere(out['ZcPhase'] > 0)]
    if len(freqs) > 0:
        ZcPeakPhaseFreq.append(out['Freq'][np.argwhere(out['ZcPhase'] == np.max(out['ZcPhase']))])
        ZcLeadPhaseBW.append(freqs[-1] - freqs[0])
        ZcLeadPhaseMinFreq.append(freqs[0])
        ZcSynchFreq.append(freqs[-1])
        ZcLeadPhaseBool.append(out['ZcPhase'])
    else:
        ZcPeakPhaseFreq.append(nan)
        ZcLeadPhaseBW.append(0)
        ZcLeadPhaseMinFreq.append(nan)
        ZcSynchFreq.append(nan)
        ZcLeadPhaseBool.append(out['ZcPhase'])
    print(str(sec) + ' ' + str(loc))
else:
    for loc in np.linspace(1/(nseg+1), nseg/(nseg+1), nseg):
        out = applyChirp(I, t, stim_sec(loc), soma_seg, t0, delay, Fs, f1)
        ZinResAmp.append(out['ZinResAmp'])
        ZinResFreq.append(out['ZinResFreq'])
        ZcResAmp.append(out['ZcResAmp'])
        ZcResFreq.append(out['ZcResFreq'])
        dist.append(out['dist'])
        QfactorIn.append(out['QfactorIn'])
        QfactorTrans.append(out['QfactorTrans'])
        fVarIn.append(out['fVarIn'])
        fVarTrans.append(out['fVarTrans'])

        freqs = out['Freq'][np.argwhere(out['ZinPhase'] > 0)]
        if len(freqs) > 0:
            ZinPeakPhaseFreq.append(out['Freq'][np.argwhere(out['ZinPhase'] == np.max(out['ZinPhase']))])
            ZinLeadPhaseBW.append(freqs[-1] - freqs[0])
            ZinLeadPhaseMinFreq.append(freqs[0])
            ZinSynchFreq.append(freqs[-1])
            ZinLeadPhaseBool.append(out['ZinPhase'] > 0)
        else:
            ZinPeakPhaseFreq.append(nan)
            ZinLeadPhaseBW.append(0)
            ZinLeadPhaseMinFreq.append(nan)
            ZinSynchFreq.append(nan)
            ZinLeadPhaseBool.append(out['ZinPhase'] > 0)

        freqs = out['Freq'][np.argwhere(out['ZcPhase'] > 0)]
        if len(freqs) > 0:
            ZcPeakPhaseFreq.append(out['Freq'][np.argwhere(out['ZcPhase'] == np.max(out['ZcPhase']))])
            ZcLeadPhaseBW.append(freqs[-1] - freqs[0])
            ZcLeadPhaseMinFreq.append(freqs[0])
            ZcSynchFreq.append(freqs[-1])
            ZcLeadPhaseBool.append(out['ZcPhase'])
        else:
            ZcPeakPhaseFreq.append(nan)
            ZcLeadPhaseBW.append(0)
            ZcLeadPhaseMinFreq.append(nan)
            ZcSynchFreq.append(nan)
            ZcLeadPhaseBool.append(out['ZcPhase'])

output = { 'ZinResAmp' : ZinResAmp,
            'ZinResFreq' : ZinResFreq,
            'ZcResAmp' : ZcResAmp,
            'ZcResFreq' : ZcResFreq,
            'dist' : dist,
            'QfactorIn' : QfactorIn,
            'QfactorTrans' : QfactorTrans,
            'fVarIn' : fVarIn,
            'fVarTrans' : fVarTrans,
            'ZinPeakPhaseFreq' : ZinPeakPhaseFreq, 
            'ZinLeadPhaseBW' : ZinLeadPhaseBW,
            'ZinLeadPhaseMinFreq' : ZinLeadPhaseMinFreq,
            'ZinSynchFreq' : ZinSynchFreq,
            'ZinLeadPhaseBool' : ZinLeadPhaseBool,
            'ZcPeakPhaseFreq' : ZcPeakPhaseFreq,
            'ZcLeadPhaseBW' : ZcLeadPhaseBW,
            'ZcLeadPhaseMinFreq' : ZcLeadPhaseMinFreq,
            'ZcSynchFreq' : ZcSynchFreq,
            'ZcLeadPhaseBool' : ZcLeadPhaseBool}

savemat('/home/craig_kelley_downstate_edu/L5PYR_Resonance/Kole/Vary_Global_Gs/ih_' + sys.argv[-3] + '_im_' + sys.argv[-2] + '/' + stim_sec.name() + '.mat', output)

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