In voltage-clamp experiments, incomplete space clamp distorts the recorded currents, rendering accurate analysis impossible. Here, we present
a simple numerical algorithm that corrects such distortions. The method enabled accurate
retrieval of the local densities, kinetics, and density gradients of somatic and dendritic channels. The correction method was applied to two-electrode voltage-clamp recordings of K currents from the apical dendrite of layer 5 neocortical pyramidal
neurons. The generality and robustness of the algorithm make it a useful tool for voltage-clamp analysis of voltage-gated
currents in structures of any morphology that is amenable to the voltage-clamp technique.
Schaefer AT, Helmstaedter M, Sakmann B, Korngreen A (2003) Correction of conductance measurements in non-space-clamped structures: 1. Voltage-gated k(+) channels. Biophys J 84:3508-28 [PubMed]
Schaefer AT, Helmstaedter M, Schmitt AC, Bar-Yehuda D, Almog M, Ben-Porat H, Sakmann B, Korng (2007) Dendritic voltage-gated K+ conductance gradient in pyramidal neurones of neocortical layer 5B from rats. J Physiol 579:737-52 [PubMed]