load_file("nrngui.hoc")
objectvar save_window_, rvp_
objectvar scene_vector_[4]
objectvar ocbox_, ocbox_list_, scene_, scene_list_
{ocbox_list_ = new List()  scene_list_ = new List()}
{pwman_place(935,30,1)}
{
xpanel("RunControl", 0)
v_init = -65
xvalue("Init","v_init", 1,"stdinit()", 1, 1 )
xbutton("Init & Run","run()")
xbutton("Stop","stoprun=1")
runStopAt = 5
xvalue("Continue til","runStopAt", 1,"{continuerun(runStopAt) stoprun=1}", 1, 1 )
runStopIn = 1
xvalue("Continue for","runStopIn", 1,"{continuerun(t + runStopIn) stoprun=1}", 1, 1 )
xbutton("Single Step","steprun()")
t = 500
xvalue("t","t", 2 )
tstop = 500
xvalue("Tstop","tstop", 1,"tstop_changed()", 0, 1 )
dt = 0.05
xvalue("dt","dt", 1,"setdt()", 0, 1 )
steps_per_ms = 20
xvalue("Points plotted/ms","steps_per_ms", 1,"setdt()", 0, 1 )
xcheckbox("Quiet",&stdrun_quiet,"")
realtime = 44
xvalue("Real Time","realtime", 0,"", 0, 1 )
xpanel(53,132)
}
{
xpanel("VClamp2[0] at bp(0.5)", 0)
xlabel("VClamp2[0] at bp(0.5)")
bpclamp.dur[0] = 0
xvalue("dur[0]","bpclamp.dur[0]", 1,"", 0, 1 )
bpclamp.dur[1] = 500
xvalue("dur[1]","bpclamp.dur[1]", 1,"", 0, 1 )
bpclamp.dur[2] = 1000
xvalue("dur[2]","bpclamp.dur[2]", 1,"", 0, 1 )
bpclamp.dur[3] = 0
xvalue("dur[3]","bpclamp.dur[3]", 1,"", 0, 1 )
bpclamp.dur[4] = 0
xvalue("dur[4]","bpclamp.dur[4]", 1,"", 0, 1 )
bpclamp.dur[5] = 0
xvalue("dur[5]","bpclamp.dur[5]", 1,"", 0, 1 )
bpclamp.amp[0] = -65
xvalue("amp[0]","bpclamp.amp[0]", 1,"", 0, 1 )
bpclamp.amp[1] = 0
xvalue("amp[1]","bpclamp.amp[1]", 1,"", 0, 1 )
bpclamp.amp[2] = -65
xvalue("amp[2]","bpclamp.amp[2]", 1,"", 0, 1 )
bpclamp.amp[3] = 0
xvalue("amp[3]","bpclamp.amp[3]", 1,"", 0, 1 )
bpclamp.amp[4] = 0
xvalue("amp[4]","bpclamp.amp[4]", 1,"", 0, 1 )
bpclamp.amp[5] = 0
xvalue("amp[5]","bpclamp.amp[5]", 1,"", 0, 1 )
bpclamp.gain = 100000
xvalue("gain","bpclamp.gain", 1,"", 0, 1 )
bpclamp.rstim = 1
xvalue("rstim","bpclamp.rstim", 1,"", 0, 1 )
bpclamp.tau1 = 0.001
xvalue("tau1","bpclamp.tau1", 1,"", 0, 1 )
bpclamp.tau2 = 0
xvalue("tau2","bpclamp.tau2", 1,"", 0, 1 )
bpclamp.e0 = 1.3444e-06
xvalue("e0","bpclamp.e0", 1,"", 0, 1 )
bpclamp.vo0 = -0.13444
xvalue("vo0","bpclamp.vo0", 1,"", 0, 1 )
bpclamp.vi0 = 2.6889e-06
xvalue("vi0","bpclamp.vi0", 1,"", 0, 1 )
bpclamp.fac = 0
xvalue("fac","bpclamp.fac", 1,"", 0, 1 )
bpclamp.i = -0.134446
xvalue("i","bpclamp.i", 0,"", 0, 1 )
bpclamp.e = 1.34443e-06
xvalue("e","bpclamp.e", 0,"", 0, 1 )
bpclamp.vo = -0.134443
xvalue("vo","bpclamp.vo", 0,"", 0, 1 )
bpclamp.vi = 2.68886e-06
xvalue("vi","bpclamp.vi", 0,"", 0, 1 )
xpanel(452,567)
}
{
save_window_ = new Graph(0)
save_window_.size(0,500,-1320,7320)
scene_vector_[2] = save_window_
{save_window_.view(0, -1320, 500, 8640, 394, 33, 451.2, 329.92)}
graphList[2].append(save_window_)
save_window_.save_name("graphList[2].")
save_window_.addvar("total_count_ribbon_ca", 1, 1, 0.229378, 0.964306, 2)
}
{
xpanel("VClamp2[1] at bp2(0.5)", 0)
xlabel("VClamp2[1] at bp2(0.5)")
bpclamp2.dur[0] = 0
xvalue("dur[0]","bpclamp2.dur[0]", 1,"", 0, 1 )
bpclamp2.dur[1] = 500
xvalue("dur[1]","bpclamp2.dur[1]", 1,"", 0, 1 )
bpclamp2.dur[2] = 1000
xvalue("dur[2]","bpclamp2.dur[2]", 1,"", 0, 1 )
bpclamp2.dur[3] = 0
xvalue("dur[3]","bpclamp2.dur[3]", 1,"", 0, 1 )
bpclamp2.dur[4] = 0
xvalue("dur[4]","bpclamp2.dur[4]", 1,"", 0, 1 )
bpclamp2.dur[5] = 0
xvalue("dur[5]","bpclamp2.dur[5]", 1,"", 0, 1 )
bpclamp2.amp[0] = -65
xvalue("amp[0]","bpclamp2.amp[0]", 1,"", 0, 1 )
bpclamp2.amp[1] = 0
xvalue("amp[1]","bpclamp2.amp[1]", 1,"", 0, 1 )
bpclamp2.amp[2] = -65
xvalue("amp[2]","bpclamp2.amp[2]", 1,"", 0, 1 )
bpclamp2.amp[3] = 0
xvalue("amp[3]","bpclamp2.amp[3]", 1,"", 0, 1 )
bpclamp2.amp[4] = 0
xvalue("amp[4]","bpclamp2.amp[4]", 1,"", 0, 1 )
bpclamp2.amp[5] = 0
xvalue("amp[5]","bpclamp2.amp[5]", 1,"", 0, 1 )
bpclamp2.gain = 100000
xvalue("gain","bpclamp2.gain", 1,"", 0, 1 )
bpclamp2.rstim = 1
xvalue("rstim","bpclamp2.rstim", 1,"", 0, 1 )
bpclamp2.tau1 = 0.001
xvalue("tau1","bpclamp2.tau1", 1,"", 0, 1 )
bpclamp2.tau2 = 0
xvalue("tau2","bpclamp2.tau2", 1,"", 0, 1 )
bpclamp2.e0 = 2.0709e-06
xvalue("e0","bpclamp2.e0", 1,"", 0, 1 )
bpclamp2.vo0 = -0.20709
xvalue("vo0","bpclamp2.vo0", 1,"", 0, 1 )
bpclamp2.vi0 = 4.1418e-06
xvalue("vi0","bpclamp2.vi0", 1,"", 0, 1 )
bpclamp2.fac = 0
xvalue("fac","bpclamp2.fac", 1,"", 0, 1 )
bpclamp2.i = -0.207093
xvalue("i","bpclamp2.i", 0,"", 0, 1 )
bpclamp2.e = 2.07088e-06
xvalue("e","bpclamp2.e", 0,"", 0, 1 )
bpclamp2.vo = -0.207088
xvalue("vo","bpclamp2.vo", 0,"", 0, 1 )
bpclamp2.vi = 4.14177e-06
xvalue("vi","bpclamp2.vi", 0,"", 0, 1 )
xpanel(715,569)
}
{
ocbox_ = new VBox()
ocbox_list_.prepend(ocbox_)
ocbox_.intercept(1)
}
{
xpanel("", 0)
RIN = 0.125
xvalue("RC IN","RIN", 1,"set_stim()", 0, 0 )
TR = 0.8
xvalue("Taur BP","TR", 1,"set_stim()", 0, 0 )
TR2 = 0.18
xvalue("Taur BP-2","TR2", 1,"set_stim()", 0, 0 )
MAXRVP = 5
xvalue("MAX RVP","MAXRVP", 1,"set_stim()", 0, 0 )
GCABP = 0.0012
xvalue("gcabar bp","GCABP", 1,"set_stim()", 0, 0 )
GCABP2 = 0.0012
xvalue("gcabar bp2","GCABP2", 1,"set_stim()", 0, 0 )
dcad1 = 0.025
xvalue("depth_cad bp","dcad1", 1,"set_stim()", 0, 0 )
dcad2 = 0.05
xvalue("depth_cad bp2","dcad2", 1,"set_stim()", 0, 0 )
xpanel()
}
{
ocbox_ = ocbox_list_.object(0)
ocbox_.intercept(0)
ocbox_.map("nrniv", 998, 335, 242.88, 252.48)
}
objref ocbox_
{
save_window_ = new Graph(0)
save_window_.size(0,500,0,1)
scene_vector_[3] = save_window_
{save_window_.view(0, 0, 500, 1, 53, 536, 300.48, 200.32)}
graphList[2].append(save_window_)
save_window_.save_name("graphList[2].")
save_window_.label(0.172524, 0.896166, "Fig. 8A", 2, 1, 0, 0, 1)
save_window_.label(0.0958466, 0.800319, "Vary the pulse-duration in the pre-synaptic", 2, 1, 0, 0, 1)
save_window_.label(0.0990415, 0.675719, "cell by adjusting dur[1] in BOTH clamp", 2, 1, 0, 0, 1)
save_window_.label(0.111821, 0.560703, "panels", 2, 1, 0, 0, 1)
save_window_.label(0.108626, 0.436102, "The paper used an average of 10 runs", 2, 1, 0, 0, 1)
save_window_.label(0.111821, 0.335463, "for each data point.", 2, 1, 0, 0, 1)
save_window_.xaxis(3)
}
objectvar scene_vector_[1]
{doNotify()}