// replace cad with cas in ganglion cell
load_file("nrngui.hoc")
load_proc ("nrnmainmenu")
load_file("gc.nrn")
create initseg, narrowr, axon, preSyn, preSyn2
initseg { nseg = int(45/15) diam=1 L=45 }
narrowr { nseg = int(90/15) diam=0.4 L=90 }
axon{ nseg = int(1000/15) diam=1 L=1000 }
connect soma(1),initseg(0)
connect initseg(1),narrowr(0)
connect narrowr(1),axon(0)
forall{
insert cas
taur_cas=1.5
}
/*
The [Ca++] is detected at two depths in the presynaptic bipolar cell.
This is implemented by two compartments which are identical
except for the depth at which the calcium shell is set.
*/
objectvar bpclamp, bpclamp2
// Pre synaptic mechanisms (L channel and Voltage Clamp)
preSyn{
insert tsbp
uninsert cas
insert cad
L=10
diam=10
depth_cad = 0.025
taur_cad=0.1
gcabar_tsbp = 0.0012
bpclamp = new VClamp(0.5)
bpclamp.dur[0]=5500
}
preSyn2{
insert tsbp
uninsert cas
insert cad
L=10
diam=10
depth_cad = 0.05
taur_cad=0.18
gcabar_tsbp = 0.0012
bpclamp2 = new VClamp(0.5)
bpclamp2.dur[0]=5500
}
// Read the voltage file and play through the Voltage Clamps
objref f1, vec, vec2
f1 = new File()
f1.ropen("bp.dat")
vec = new Vector()
NumScan=vec.scanf(f1,192000,1,1)
f1.close()
vec2 = new Vector()
vec2 = vec.c
vec.play(&bpclamp.amp[0],0.025)
vec2.play(&bpclamp2.amp[0],0.025)
//////////
forall{ // except the bipolar cells which are uninserted below
insert spike
// dendritic channel densities
gcabar_spike = 0.002
gkbar_spike = 0.012
gabar_spike = 0.036
gkcbar_spike = 0.000001
gnabar_spike = 0.025
}
soma{
insert spike
gcabar_spike = 0.0015
gkbar_spike = 0.018
gabar_spike = 0.054
gkcbar_spike = 0.000065
gnabar_spike = 0.10
}
initseg {
insert spike
gcabar_spike = 0.0015
gkbar_spike = 0.018
gabar_spike = 0.054
gkcbar_spike = 0.000065
gnabar_spike = 0.125
}
narrowr {
insert spike
gcabar_spike = 0.0
gkbar_spike = 0.018
gabar_spike = 0.054
gkcbar_spike = 0.000065
gnabar_spike = 0.1
}
axon {
insert spike
gcabar_spike = 0.0
gkbar_spike = 0.018
gabar_spike = 0.0
gkcbar_spike = 0.000065
gnabar_spike = 0.07
}
forall{
mod_modulator = 0
ena=35
ek=-75
cao=1.8
cai=.0001
insert pas
g_pas= 1/70000
Ra=110
global_ra=110
e_pas=-65
}
access preSyn
uninsert spike
access preSyn2
uninsert spike
/* Synapses are positioned onto ganglion cell and connected presynaptically to bipolars
Each synapse has at its disposal the presynaptic [Ca++] at two depths, but at any
timestep only uses one in determining the rate constant.
*/
objectvar synapse[23]
for i=0, 11{
access dend1[i]
synapse[i] = new ribbon_tiger(0.5)
setpointer synapse[i].preCA1,preSyn.cai(0.5)
setpointer synapse[i].preCA2,preSyn2.cai(0.5)
synapse[i].gmax=0.00128
synapse[i].gmaxN=0.0001
synapse[i].mg = 0.5
}
objectvar synapse2[23]
for i=0, 11{
access dend1[i]
synapse2[i] = new ribbon_tiger(0.5)
setpointer synapse2[i].preCA1,preSyn.cai(0.5)
setpointer synapse2[i].preCA2,preSyn2.cai(0.5)
synapse2[i].gmax=0.00128
synapse[i].gmaxN=0.0001
synapse2[i].mg = 0.5
}
objectvar synapse3[23]
for i=0, 11{
access dend1[i]
synapse3[i] = new ribbon_tiger(0.5)
setpointer synapse3[i].preCA1,preSyn.cai(0.5)
setpointer synapse3[i].preCA2,preSyn2.cai(0.5)
synapse3[i].gmax=0.00128
synapse3[i].gmaxN=0.0001
synapse3[i].mg = 0.5
}
access dend1[19]
synapse[12] = new ribbon_tiger(0.5)
setpointer synapse[12].preCA1,preSyn.cai(0.5)
setpointer synapse[12].preCA2,preSyn2.cai(0.5)
access dend2[0]
synapse[13] = new ribbon_tiger(0.5)
setpointer synapse[13].preCA1,preSyn.cai(0.5)
setpointer synapse[13].preCA2,preSyn2.cai(0.5)
access dend2[1]
synapse[14] = new ribbon_tiger(0.5)
setpointer synapse[14].preCA1,preSyn.cai(0.5)
setpointer synapse[14].preCA2,preSyn2.cai(0.5)
access dend2[10]
synapse[15] = new ribbon_tiger(0.5)
setpointer synapse[15].preCA1,preSyn.cai(0.5)
setpointer synapse[15].preCA2,preSyn2.cai(0.5)
access dend3[0]
synapse[16] = new ribbon_tiger(0.5)
setpointer synapse[16].preCA1,preSyn.cai(0.5)
setpointer synapse[16].preCA2,preSyn2.cai(0.5)
access dend1[22]
synapse[17] = new ribbon_tiger(0.5)
setpointer synapse[17].preCA1,preSyn.cai(0.5)
setpointer synapse[17].preCA2,preSyn2.cai(0.5)
access dend4[0]
synapse[18] = new ribbon_tiger(0.5)
setpointer synapse[18].preCA1,preSyn.cai(0.5)
setpointer synapse[18].preCA2,preSyn2.cai(0.5)
access dend5[0]
synapse[19] = new ribbon_tiger(0.5)
setpointer synapse[19].preCA1,preSyn.cai(0.5)
setpointer synapse[19].preCA2,preSyn2.cai(0.5)
access dend6[0]
synapse[20] = new ribbon_tiger(0.5)
setpointer synapse[20].preCA1,preSyn.cai(0.5)
setpointer synapse[20].preCA2,preSyn2.cai(0.5)
access dend6[1]
synapse[21] = new ribbon_tiger(0.5)
setpointer synapse[21].preCA1,preSyn.cai(0.5)
setpointer synapse[21].preCA2,preSyn2.cai(0.5)
access dend6[2]
synapse[22] = new ribbon_tiger(0.5)
setpointer synapse[22].preCA1,preSyn.cai(0.5)
setpointer synapse[22].preCA2,preSyn2.cai(0.5)
access dend1[19]
synapse2[12] = new ribbon_tiger(0.5)
setpointer synapse2[12].preCA1,preSyn.cai(0.5)
setpointer synapse2[12].preCA2,preSyn2.cai(0.5)
access dend2[0]
synapse2[13] = new ribbon_tiger(0.5)
setpointer synapse2[13].preCA1,preSyn.cai(0.5)
setpointer synapse2[13].preCA2,preSyn2.cai(0.5)
access dend2[1]
synapse2[14] = new ribbon_tiger(0.5)
setpointer synapse2[14].preCA1,preSyn.cai(0.5)
setpointer synapse2[14].preCA2,preSyn2.cai(0.5)
access dend2[10]
synapse2[15] = new ribbon_tiger(0.5)
setpointer synapse2[15].preCA1,preSyn.cai(0.5)
setpointer synapse2[15].preCA2,preSyn2.cai(0.5)
access dend3[0]
synapse2[16] = new ribbon_tiger(0.5)
setpointer synapse2[16].preCA1,preSyn.cai(0.5)
setpointer synapse2[16].preCA2,preSyn2.cai(0.5)
access dend1[22]
synapse2[17] = new ribbon_tiger(0.5)
setpointer synapse2[17].preCA1,preSyn.cai(0.5)
setpointer synapse2[17].preCA2,preSyn2.cai(0.5)
access dend4[0]
synapse2[18] = new ribbon_tiger(0.5)
setpointer synapse2[18].preCA1,preSyn.cai(0.5)
setpointer synapse2[18].preCA2,preSyn2.cai(0.5)
access dend5[0]
synapse2[19] = new ribbon_tiger(0.5)
setpointer synapse2[19].preCA1,preSyn.cai(0.5)
setpointer synapse2[19].preCA2,preSyn2.cai(0.5)
access dend6[0]
synapse2[20] = new ribbon_tiger(0.5)
setpointer synapse2[20].preCA1,preSyn.cai(0.5)
setpointer synapse2[20].preCA2,preSyn2.cai(0.5)
access dend6[1]
synapse2[21] = new ribbon_tiger(0.5)
setpointer synapse2[21].preCA1,preSyn.cai(0.5)
setpointer synapse2[21].preCA2,preSyn2.cai(0.5)
access dend6[2]
synapse2[22] = new ribbon_tiger(0.5)
setpointer synapse2[22].preCA1,preSyn.cai(0.5)
setpointer synapse2[22].preCA2,preSyn2.cai(0.5)
access dend1[19]
synapse3[12] = new ribbon_tiger(0.5)
setpointer synapse3[12].preCA1,preSyn.cai(0.5)
setpointer synapse3[12].preCA2,preSyn2.cai(0.5)
access dend2[0]
synapse3[13] = new ribbon_tiger(0.5)
setpointer synapse3[13].preCA1,preSyn.cai(0.5)
setpointer synapse3[13].preCA2,preSyn2.cai(0.5)
access dend2[1]
synapse3[14] = new ribbon_tiger(0.5)
setpointer synapse3[14].preCA1,preSyn.cai(0.5)
setpointer synapse3[14].preCA2,preSyn2.cai(0.5)
access dend2[10]
synapse3[15] = new ribbon_tiger(0.5)
setpointer synapse3[15].preCA1,preSyn.cai(0.5)
setpointer synapse3[15].preCA2,preSyn2.cai(0.5)
access dend3[0]
synapse3[16] = new ribbon_tiger(0.5)
setpointer synapse3[16].preCA1,preSyn.cai(0.5)
setpointer synapse3[16].preCA2,preSyn2.cai(0.5)
access dend1[22]
synapse3[17] = new ribbon_tiger(0.5)
setpointer synapse3[17].preCA1,preSyn.cai(0.5)
setpointer synapse3[17].preCA2,preSyn2.cai(0.5)
access dend4[0]
synapse3[18] = new ribbon_tiger(0.5)
setpointer synapse3[18].preCA1,preSyn.cai(0.5)
setpointer synapse3[18].preCA2,preSyn2.cai(0.5)
access dend5[0]
synapse3[19] = new ribbon_tiger(0.5)
setpointer synapse3[19].preCA1,preSyn.cai(0.5)
setpointer synapse3[19].preCA2,preSyn2.cai(0.5)
access dend6[0]
synapse3[20] = new ribbon_tiger(0.5)
setpointer synapse3[20].preCA1,preSyn.cai(0.5)
setpointer synapse3[20].preCA2,preSyn2.cai(0.5)
access dend6[1]
synapse3[21] = new ribbon_tiger(0.5)
setpointer synapse3[21].preCA1,preSyn.cai(0.5)
setpointer synapse3[21].preCA2,preSyn2.cai(0.5)
access dend6[2]
synapse3[22] = new ribbon_tiger(0.5)
setpointer synapse3[22].preCA1,preSyn.cai(0.5)
setpointer synapse3[22].preCA2,preSyn2.cai(0.5)
GMAX = 0.00256
GMAXN = 0.00011
ABSR=0
proc set_stim(){ // Used by the control panel defined below
for i = 0, 22 {
synapse[i].gmax = GMAX
synapse[i].gmaxN = GMAXN
synapse[i].AbsRefract = ABSR
synapse[i].mg = 0.5
}
for i= 0, 22 {
synapse2[i].gmax = GMAX
synapse2[i].gmaxN = GMAXN
synapse2[i].AbsRefract = ABSR
synapse2[i].mg = 0.5
}
for i = 0, 22 {
synapse3[i].gmax = GMAX
synapse3[i].gmaxN = GMAXN
synapse3[i].AbsRefract = ABSR
synapse3[i].mg = 0.5
}
}
set_stim()
objectvar vbox,vbox3, tempobj3
proc receptor_panel(){
vbox3 = new VBox()
vbox3.intercept(1)
xpanel("Single Release Site")
xvalue("dAMPA gmax","GMAX",1, "set_stim()")
xvalue("NMDA gmax","GMAXN",1, "set_stim()")
// xvalue("Absolute Refractory", "ABSR",1, "set_stim()")
xpanel()
vbox3.intercept(0)
vbox3.map
}
access soma
objectvar soma_clamp
soma_clamp = new VClamp(0.5)
xopen("tiger.ses")
Simulation Platform