163. Lüthi A, Gähwiler BH and Gerber U. (1997) 1S, 3R-ACPD induces a region of negative slope conductance in the steady-state current-voltage relationship of hippocampal pyramidal cells. J Neurophysiol 77:221-8.

NeuronCompartmentPropertyConnectivityNotes
Hippocampus CA3 pyramidal cellMiddle apical dendritemGluRReceptor Notes Dentate Granule Cell mossy terminals (T) Intracellular recording from CA3 pyramidal neurons in a slice culture from rat found a slow excitatory response to Glu application in the presence of blocking agents for the ionotropic GluRs. Further experimentation revealed that ACPD could evoke the same response, which was due to the depression of I(K, Ca) and voltage-gated I(K) (Charpak S et al, 1990151 ). A subsequent study in rat slice cultures has shown that bath application of MCPG (a mGluR antagonist) blocks the inward Ca-dependent K-current associated with ACPD application or mossy fiber stimulation in the presence on ionotropic GluR antagonists (Gerber U et al, 1993 [rat ]155 ). Another study using bath application of 1S,3R-ACPD in rat slice cultures during single electrode voltage clamp recording showed that depolarizing current steps revealed a suppression of K currents leading to a negative slope conductance at potential between -55mV and -40 mV (Lüthi A et al, 1997163 ). mGluR2 knockout mice show normal LTP and synaptic transmission but not LTD (Yokoi M et al, 1996 [mice ]175 ). It has also been shown (using whole and perforated patch recording from acutely isolated CA3 pyramidal neurons) that application of Glu and quisqualatic acid (in the presence of D-AP5, an NMDAR-antagonist, and CNQX, an AMPAR antagonist) results in responses that consist of an inward current that may be preceded by an outward current. Both of these currents are affected by bath [K] and they had different pharmacological properties (Harata et al, 1996).
Hippocampus CA3 pyramidal cellSomamGluR-----Intracellular recording from CA3 pyramidal neurons in a slice culture from rat found a slow excitatory response to Glu application in the presence of blocking agents for the ionotropic GluRs. Further experimentation revealed that ACPD could evoke the same response, which was due to the depression of I(K,Ca) and voltage-gated I(K) (Charpak S et al, 1990151 ). A subsequent study in rat slice cultures has shown that bath application of MCPG (a mGluR antagonist) blocks the inward Ca-dependent K-current associated with ACPD application or mossy fiber stimulation in the presence on ionotropic GluR antagonists (Gerber U et al, 1993 [rat ]155 ). Another study using bath application of 1S,3R-ACPD in rat slice cultures during single electrode voltage clamp recording showed that depolarizing current steps revealed a suppression of K currents leading to a negative slope conductance at potential between -55mV and -40 mV (Lüthi A et al, 1997163 ). mGluR2 knockout mice show normal LTP and synaptic transmission but not LTD (Yokoi M et al, 1996 [mice ]175 ). It has also been shown (using whole cell and perforated patch recording from acutely isolated CA3 pyramidal neurons) that application of Glu and quisqualatic acid (in the presence of D-AP5, an NMDAR-antagonist, and CNQX, an AMPAR antagonist) results in responses that consist of an inward current that may be preceded by an outward current. Both of these currents are affected by bath [K] and they had different pharmacological properties (Harata et al. 1996 #8680866).

References
151. Charpak S, Gahwiler BH, Do KQ and Knopfel T. (1990) Potassium conductances in hippocampal neurons blocked by excitatory amino-acid transmitters. Nature 347:765-7.
155. Gerber U, Lüthi A and Gähwiler BH. (1993) Inhibition of a slow synaptic response by a metabotropic glutamate receptor antagonist in hippocampal CA3 pyramidal cells. Proc Biol Sci 254:169-72 [Journal] .
163. Lüthi A, Gähwiler BH and Gerber U. (1997) 1S, 3R-ACPD induces a region of negative slope conductance in the steady-state current-voltage relationship of hippocampal pyramidal cells. J Neurophysiol 77:221-8.
175. Yokoi M, Kobayashi K, Manabe T, Takahashi T, Sakaguchi I, Katsuura G, Shigemoto R, Ohishi H, Nomura S, Nakamura K, Nakao K, Katsuki M and Nakanishi S. (1996) Impairment of hippocampal mossy fiber LTD in mice lacking mGluR2. Science 273:645-7.
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