170. Monaghan DT, Yao D and Cotman CW. (1984) Distribution of [3H]AMPA binding sites in rat brain as determined by quantitative autoradiography. Brain Res 324:160-4.

NeuronCompartmentPropertyConnectivityNotes
Hippocampus CA3 pyramidal cellDistal apical dendriteAMPAPerforant pathway entorhinal pyramidal neuron terminals (T)Quantitative autoradiography has been used to localize [3H]AMPA binding sites. It was found that AMPARs are found in a high concentration in the hippocampus relative to other areas in the brain. In CA3, labeling was substantially heavier in s. pyramidale than in s.radiatum and s. lacunosum-moleculare (Monaghan DT et al, 1984 [rat ]170 ). The physiology of these receptors has been studied in outside-out patches from the proximal apical dendrites. It was found that a CNQX-sensitive component of the synaptic current evoked by fast aplication of glutamate could be isolated (and was presumed to be the result of AMPA channel opening). It was calculated that AMPA channels had a mean elementary conductance of 10 pS (estimated by non-stationary fluctuation analysis) and was found that the channels had a low permeability to Ca2+. The reversal potential for AMPA receptors was found to be about 0 mV with an almost linear peak current-voltage relationship (Spruston N et al, 1995 [rat ]174 ; see also Jahr CE and Stevens CF, 2000 [rat ]159 ). It has also been found that CNQX does not block the intracellular calcium concentration increase normally associated with stratum lucidum stimulation (Pozzo Miller LD et al, 1996168 ). Recordings from membrane patches of dendrites and soma reveal fast and slow responses to fast application of glutamate, mediated by AMPA amd NMDA receptors, respectively (Spruston N et al, 1995434 ).
Hippocampus CA3 pyramidal cellMiddle apical dendriteAMPACA3 Pyramidal neuron terminals (T)Quantitative autoradiography has been used to localize [3H]AMPA binding sites. It was found that AMPARs are found in a high concentration in the hippocampus relative to other areas in the brain. In CA3, labeling was substantially heavier in s. pyramidale than in s.radiatum and s. lacunosum-moleculare (Monaghan DT et al, 1984 [rat ]170 ). The physiology of these receptors has been studied in outside-out patches from the proximal apical dendrites. It was found that a CNQX-sensitive component of the synaptic current evoked by fast aplication of glutamate could be isolated (and was presumed to be the result of AMPA channel opening). It was calculated that AMPA channels had a mean elementary conductance of 10 pS (estimated by non-stationary fluctuation analysis) and was found that the channels had a low permeability to Ca2+. The reversal potential for AMPA receptors was found to be about 0 mV with an almost linear peak current-voltage relationship (Spruston N et al, 1995 [rat ]174 ; see also Jahr CE and Stevens CF, 2000 [rat ]159 ). It has also been found that CNQX does not block the intracellular calcium concentration increase normally associated with stratum lucidum stimulation (Sheng M et al, 1994 [rat ]173 ).
Hippocampus CA3 pyramidal cellSomaAMPA-----Quantitative autoradiography has been used to localize [3H]AMPA binding sites. In CA3, labeling was substantially heavier in s. pyramidale than in s.radiatum and s. lacunosum-moleculare (Monaghan DT et al, 1984 [rat ]170 ). [ed. note: we are not aware of glutamatergic synapses onto the soma] Recordings from membrane patches of dendrites and soma reveal fast and slow responses to fast application of glutamate, mediated by AMPA amd NMDA receptors, respectively (Spruston N et al, 1995434 ).

References
170. Monaghan DT, Yao D and Cotman CW. (1984) Distribution of [3H]AMPA binding sites in rat brain as determined by quantitative autoradiography. Brain Res 324:160-4.
174. Spruston N, Schiller Y, Stuart G and Sakmann B. (1995) Activity-dependent action potential invasion and calcium influx into hippocampal CA1 dendrites. Science 268:297-300.
159. Jahr CE and Stevens CF. (2000) Glutamate activates multiple single channel conductances in hippocampal neurons. Nature 325:522-5.
168. Pozzo Miller LD, Petrozzino JJ, Golarai G and Connor JA. (1996) Ca2+ release from intracellular stores induced by afferent stimulation of CA3 pyramidal neurons in hippocampal slices. J Neurophysiol 76:554-62.
434. Spruston N, Jonas P and Sakmann B. (1995) Dendritic glutamate receptor channels in rat hippocampal CA3 and CA1 pyramidal neurons. J Physiol 482 ( Pt 2):325-52.
173. Sheng M, Tsaur ML, Jan YN and Jan LY. (1994) Contrasting subcellular localization of the Kv1.2 K+ channel subunit in different neurons of rat brain. J Neurosci 14:2408-17.
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