Single excitatory axons form clustered synapses onto CA1 pyramidal cell dendrites (Bloss et al 2018)

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Accession:237728
" ... Here we show that single presynaptic axons form multiple, spatially clustered inputs onto the distal, but not proximal, dendrites of CA1 pyramidal neurons. These compound connections exhibit ultrastructural features indicative of strong synapses and occur much more commonly in entorhinal than in thalamic afferents. Computational simulations revealed that compound connections depolarize dendrites in a biophysically efficient manner, owing to their inherent spatiotemporal clustering. ..."
Reference:
1 . Bloss EB, Cembrowski MS, Karsh B, Colonell J, Fetter RD, Spruston N (2018) Single excitatory axons form clustered synapses onto CA1 pyramidal cell dendrites. Nat Neurosci 21:353-363 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism:
Cell Type(s): Hippocampus CA1 pyramidal GLU cell;
Channel(s):
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s):
Implementer(s):
Search NeuronDB for information about:  Hippocampus CA1 pyramidal GLU cell;
/
BlossEtAl2018
readme.html
dists.mod *
eff.mod *
gScale.mod
id.mod *
kad.mod *
kap.mod *
kdr.mod *
na3.mod *
nmdaSyn.mod
syns.mod *
addChannels.hoc *
addSpines.hoc
addSynapses.hoc
buildCell.hoc
channelParameters.hoc *
createBiophysics.hoc
createMorphology.hoc
createPlots.hoc
createPointers.hoc
doAnalysis.hoc
getBranchOrder.hoc *
idMorph.hoc
initializationAndRun.hoc
loadMorph.hoc
mosinit.hoc *
processMorph.hoc
proofreadMorph.hoc
resetNSeg.hoc *
screenshot.png
simParameters.hoc
singleSim.hoc
singleSimDist.hoc
spineGeom.hoc
spineShaftConc.hoc
start.hoc
trackVoltages.hoc
twinApical.swc *
varyDistribution.hoc
varySpaceTime.hoc
                            
////////////////////////////////////////////////////////////////////////////////
//
// Mark Cembrowski
// Janelia Research Campus
// June 4 2017
//
// Correspondence: cembrowskim@janelia.hhmi.org
//
// The following scripts examine cooperativity of inputs onto stylized and 
// realistic CA1 pyramidal cells. For some simulations, outputs are visualised
// in the NEURON environment. For others (typically, large-scale simulations), 
//
////////////////////////////////////////////////////////////////////////////////

// LOAD GUI.
{
	load_file("nrngui.hoc")
}

// ENTER IN PARAMETERS IMPORTANT FOR SIMULATION.
{
	// Choose simulation.
	theSim = 3
		// =1: integration of AMPA-based input onto stylized cell, 1 trial
		// =2: integration of AMPA-based input onto stylized cell, many trials
		// =3: integration of AMPA and NMDA-based input onto complex cell,
		//		1 trial
		// =4: integration of AMPA and NMDA-based input onto complex cell,
		//		many trials
		
	// Adjust scaling of synaptic strength for each synapse, if desired.
	// Note: this are overriden in buildCell.hoc if theSim=2,4, as these
	// simulations by definition are designed to look at the interaction of
	// two synapses.
	scaleAmp1 = 1
	scaleAmp2 = 0
		
	// Choose branch for synapses to be allocated to. This only applies to 
	// complex morphology, as the stylized morphology always has spines on the
	// only dendrite.
	dendInd = 105 
		
	// Choose location and temporal offset of synapses.
	dendLoc1 = 0.5 // location for first (reference) synapse
	dendLoc2 = 0.5  // location for second (variable) synapse. Note that this
				   // position is overrided for "many trials" simulation option.
	dt = 0		   // in msec, offset between two synapses.
}
// ENTER IN PARAMETERS ONLY SWITCHED RARELY. 

objref shaftInputs

{
	// Switch synapse location from spine to shaft? 
	// Separate param for each synapse. Assumes n=2 synapses.
	// Set =1 if switching. 
	// Note: this will rarely be case.
	shaftInputs = new Vector(2)
	shaftInputs.x[0] = 0
	shaftInputs.x[1] = 0
}

// CONSTRUCT INTRINSIC PROPERTIES OF CELL.
{
	load_file("buildCell.hoc")
}

// ADD SYNAPSES.
{
	load_file("addSynapses.hoc")
}

// DEFINE INITIALIZATION AND RUN, SET NUMERICAL PARAMETERS.
{
	load_file("initializationAndRun.hoc")
}


// TRACK VOLTAGES.
{
	load_file("trackVoltages.hoc")
}

// RUN AND ANALYSE.
{
	if(theSim==1){load_file("singleSim.hoc")}
	if(theSim==2){load_file("varySpaceTime.hoc")}
	if(theSim==3){load_file("singleSim.hoc")}
	if(theSim==4){load_file("varySpaceTime.hoc")}
}

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