Signaling pathways In D1R containing striatal spiny projection neurons (Blackwell et al 2018)

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Accession:239744
We implemented a mechanistic model of signaling pathways activated by dopamine D1 receptors, acetylcholine receptors, and glutamate. We use our novel, computationally efficient simulator, NeuroRD, to simulate stochastic interactions both within and between dendritic spines. Results show that the combined activity of several key plasticity molecules correctly predicts the occurrence of either LTP, LTD or no plasticity for numerous experimental protocols.
Reference:
1 . Blackwell KT, Salinas AG, Tewatia P, English B, Hellgren Kotaleski J, Lovinger DM (2019) Molecular mechanisms underlying striatal synaptic plasticity: relevance to chronic alcohol consumption and seeking. Eur J Neurosci 49:768-783 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Molecular Network; Synapse;
Brain Region(s)/Organism: Basal ganglia; Striatum;
Cell Type(s): Neostriatum medium spiny direct pathway GABA cell;
Channel(s):
Gap Junctions:
Receptor(s): D1; mGluR5; M1; M4;
Gene(s):
Transmitter(s): Acetylcholine; Dopamine; Endocannabinoid; Glutamate;
Simulation Environment: NeuroRD;
Model Concept(s): G-protein coupled; Long-term Synaptic Plasticity; Signaling pathways; Alcohol Use Disorder;
Implementer(s): Blackwell, Avrama [avrama at gmu.edu];
Search NeuronDB for information about:  Neostriatum medium spiny direct pathway GABA cell; M1; M4; mGluR5; D1; Acetylcholine; Dopamine; Glutamate; Endocannabinoid;
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Stim20hzACh-sp2other.xml
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StimDend_DaBath.xml
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StimTheta10hzACh-sp1.xml
StimTheta10hzACh-sp2adj.xml
StimTheta10hzACh-sp2other.xml
StimTheta10hzACh-sp2sep.xml
StimTheta10hzACh-sp3sep.xml
                            
<StimulationSet>


    <!-- the injectionSite must match a regionLabel defined in the morphology  -->
   
    <!-- one dendrite injection, sa2[:], is distributed over all the spine
	 (each spine gets total/num spines) 
	 four Glu and Ca pulses at 50 Hz, repeated 10x= 40 pulses per train -->

  <!-- This Da is single pulse, representing 4 pulse burst, burst 1 amp = 2x 1st pulse of 20 Hz
    longer duration of inject provides for the >100msegion rise time-->
  <!-- the 15 s repetition of train provides the first burst for each of 10 trains -->
  <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            60              </duration>
        <rate>                6.0           </rate>
        <period>              105             </period>
        <end>                 20100          </end>
  </InjectionStim>

  <!-- These next Da are single pulse, represent 4 pulse burst; burst 2-10 amp = 1/2 amp of 1st burst -->
  <!-- the 15 s repetition of train provides bursts 2-10 for each of 10 trains -->
  <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               20095          </onset>
        <duration>            60              </duration>
        <rate>                2.5           </rate>
        <period>              95             </period>
        <end>                 20949          </end>
  </InjectionStim>

  <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            60              </duration>
        <rate>                4.0           </rate>
        <period>              105             </period>
        <end>                 35100          </end>
        <numTrains>           9             </numTrains>
        <interTrainInterval>  14900             </interTrainInterval>    
  </InjectionStim>

  <!-- These next Da are single pulse, represent 4 pulse burst; burst 2-10 amp = 1/2 amp of 1st burst -->
  <!-- the 15 s repetition of train provides bursts 2-10 for each of 10 trains -->
  <InjectionStim specieID="DaDbuf" injectionSite="sa1[0].pointA">
        <onset>               35095          </onset>
        <duration>            60              </duration>
        <rate>                1.5           </rate>
        <period>              95             </period>
        <end>                 35949          </end>
        <numTrains>           9             </numTrains>
        <interTrainInterval>  14051             </interTrainInterval>    
  </InjectionStim>

<!-- basal ACh value prior to and after stim  -->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               0          </onset>
        <duration>            21000      </duration>
        <rate>                0.2         </rate>
</InjectionStim>
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               170000          </onset>
        <duration>            200000      </duration>
        <rate>                0.2         </rate>
</InjectionStim>
<!-- basal ACh stim paused during Da stim-->
<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               23200          </onset>
        <duration>            12800      </duration>
        <rate>                0.2         </rate>
        <period>              15000             </period>
        <end>                 170000          </end>
</InjectionStim>

 <!-- burst during stim specified for each Glu/Da train (below)-->
 <!-- rebound burst: develops more slowly -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               22400          </onset>
        <duration>            800            </duration>
        <rate>                0            </rate>
        <period>              900           </period>
        <end>                 23299          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  14101          </interTrainInterval>  
</InjectionStim>


   <!-- *****************TRAIN 1 *** Glu inject **********-->   
  <InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
  </InjectionStim>

<!--  Ca pulses TRAIN 1:  40 Ca pulses -->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               20000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               20000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               20000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 20066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--**********Glu *******TRAIN 2************-->
  <InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>  
  </InjectionStim>
  
<!--**********Calcium******TRAIN 2******* 80 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               35000          </onset>
        <duration>            3              </duration>
        <rate>               156            </rate>
        <period>              20             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               35000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               35000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 35066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 3***************-->
  <InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               50000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
  </InjectionStim>
  
<!--**********Calcium******TRAIN 3******* 120 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               50000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>      
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               50000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>   
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               50000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               50000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 50066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--**********Glu******TRAIN 4******************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               65000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>
  
<!--**********Calcium******TRAIN 4******* 160 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               65000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval> 
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               65000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval> 
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               65000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               65000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 65066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--*********Glu******TRAIN 5***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               80000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>  
</InjectionStim>

<!--**********Calcium******TRAIN 5******* 200 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               80000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               80000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               80000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               80000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 80066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--**********Glu******TRAIN 6******************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>               95000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 6******* 240 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>               95000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>               95000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>   
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               95000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               95000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 95066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 7***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              110000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 7******* 280 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              110000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              110000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               110000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               110000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 110066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 8***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              125000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 8******* 320 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              125000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>      
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              125000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               125000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               125000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 125066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


<!--*********Glu******TRAIN 9***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              140000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>    
</InjectionStim>

<!--**********Calcium******TRAIN 9******* 360 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              140000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              140000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               140000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               140000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 140066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>

<!--*********Glu******TRAIN 10***************-->
<InjectionStim specieID="GluGbuf" injectionSite="sa1[0].pointA">
        <onset>              155000          </onset>
        <duration>            5              </duration>
        <rate>                1.125           </rate>
        <period>              20             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>       
      </InjectionStim>
      
<!--**********Calcium******TRAIN 10******* 320 total Ca pulses ***********-->

<InjectionStim specieID="Ca" injectionSite="sa1[0].pointA">
        <onset>              155000          </onset>
        <duration>            3              </duration>
        <rate>                154            </rate>
        <period>              20             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>

<InjectionStim specieID="Ca" injectionSite="dend:submembrane">
        <onset>              155000          </onset>
        <duration>            3              </duration>
        <rate>                156            </rate>
        <period>              20             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29             </interTrainInterval>     
</InjectionStim>

<!-- ACh neurons fire only 1 of every two 50 Hz stim -->
<InjectionStim specieID="ACh" injectionSite="sa1[0].pointA">
        <onset>               155000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
</InjectionStim>

<InjectionStim specieID="ACh" injectionSite="dend:submembrane">
        <onset>               155000          </onset>
        <duration>            15              </duration>
        <rate>               0.0          </rate>
        <period>              40             </period>
        <end>                 155066          </end>
        <numTrains>           10             </numTrains>
        <interTrainInterval>  29          </interTrainInterval>  
 </InjectionStim>


</StimulationSet>

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