Cerebellum Purkinje cell: dendritic ion channels activated by climbing fibre (Ait Ouares et al 2019)

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Accession:244679
"In cerebellar Purkinje neuron (PN) dendrites, the transient depolarisation associated with a climbing fibre (CF) EPSP activates voltage-gated Ca2+ channels (VGCCs), voltage-gated K+ channels (VGKCs) and Ca2+ activated SK and BK K+ channels. The resulting membrane potential (Vm) and Ca2+ transients play a fundamental role in dendritic integration and synaptic plasticity of parallel fibre inputs. Here we report a detailed investigation of the kinetics of dendritic Ca2+ and K+ channels activated by CF-EPSPs, based on optical measurements of Vm and Ca2+ transients and on a single-compartment NEURON model reproducing experimental data. ... "
Reference:
1 . Ait Ouares K, Filipis L, Tzilivaki A, Poirazi P, Canepari M (2019) Two Distinct Sets of Ca2+ and K+ Channels Are Activated at Different Membrane Potentials by the Climbing Fiber Synaptic Potential in Purkinje Neuron Dendrites. J Neurosci 39:1969-1981 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Dendrite;
Brain Region(s)/Organism: Cerebellum;
Cell Type(s): Cerebellum Purkinje GABA cell;
Channel(s): I Calcium; I K,Ca; I Potassium;
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s): Simplified Models; Calcium dynamics; Active Dendrites;
Implementer(s): Filipis, Luiza [luizafilipu at gmail.com];
Search NeuronDB for information about:  Cerebellum Purkinje GABA cell; I K,Ca; I Calcium; I Potassium;
load_file("nrngui.hoc")

create  dend
access dend


 nseg = 1
 L = 20
 diam = 4
 cm=1.5


   insert climbing
       del_climbing=2 
       g_climbing = 0.002 
       icin_climbing=-0.016 
       e_climbing=-74
       tauC_climbing=10 
       tauO_climbing=0.4 
       delf_climbing=2 
       ef_climbing=-74
       tauCf_climbing=10 
       tauOf_climbing=10 
       fastfact_climbing=0
       

   insert leak
       gbar_leak =9e-5
   

     
   insert CaT3_1
       pcabar_CaT3_1=0.000 
       t1_CaT3_1=0.6
        t2_CaT3_1=55 


      insert KA1
       gkbar_KA1=0.03 
       vh1_KA1=50 
       vm1_KA1=27 
       vm2_KA1=30 



     insert newCaP1
       pcabar_newCaP1=0.000 
       pp_newCaP1=0.75

       

  
   insert Kdr1
     gkbar_Kdr1=0.092
     pp_Kdr1=8
     pdr_Kdr1=0.4
     vsh1_Kdr1=0
     vsh2_Kdr1=0
     hdr_Kdr1=1

   
   insert KC3
       gkbar_KC3=4.800
       tin_KC3=4

    insert SK2
       gkbar_SK2 =0.005   


   insert cdp5
        Nannuli_cdp5 = 0.326 + (1.94 * diam ) + (0.289*diam *diam ) - ((3.33e-2)*diam *diam *diam ) + ((1.55e-3)*diam *diam *diam *diam ) - (2.55e-5*diam *diam *diam *diam *diam )
        Buffnull2_cdp5  = 64.2 - 57.3*exp(-diam /1.4)
        rf3_cdp5  = 0.162 - 0.106*exp(-diam /2.29)
        if (diam >=2) {
                rf4_cdp5  = 0.000267 + 0.0167*exp(-diam /0.722) + 0.0028*exp(-diam /4)
        } else {
                rf4_cdp5  = 0.003
        }


//Pump
     kpmp1_cdp5    = 3e-3       
     kpmp2_cdp5   = 1.75e-5   
     kpmp3_cdp5   = 7.255e-5  
     TotalPump_cdp5 = 1e-9    

    //Dye Fura-ff
     BTCnull_cdp5 =1     
     b1_cdp5 =570   
     b2_cdp5 = 3.5 

    //Buffer
     DMNPEnull_cdp5=0.75
     c1_cdp5=570
     c2_cdp5=4

    //Calbindin
     CBnull_cdp5=0.16
     nf1_cdp5=43.5
     nf2_cdp5=3.58e-2
     ns1_cdp5=5.5
     ns2_cdp5=0.26e-2

    //Parvalbumin
     PVnull_cdp5=0.075
     m1_cdp5=107
     m2_cdp5=9.5e-4
     p1_cdp5=0.8
     p2_cdp5=2.5e-2

global_ra = 250
set_ra()
celsius=37

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