Cerebellum Purkinje cell: dendritic ion channels activated by climbing fibre (Ait Ouares et al 2019)

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Accession:244679
"In cerebellar Purkinje neuron (PN) dendrites, the transient depolarisation associated with a climbing fibre (CF) EPSP activates voltage-gated Ca2+ channels (VGCCs), voltage-gated K+ channels (VGKCs) and Ca2+ activated SK and BK K+ channels. The resulting membrane potential (Vm) and Ca2+ transients play a fundamental role in dendritic integration and synaptic plasticity of parallel fibre inputs. Here we report a detailed investigation of the kinetics of dendritic Ca2+ and K+ channels activated by CF-EPSPs, based on optical measurements of Vm and Ca2+ transients and on a single-compartment NEURON model reproducing experimental data. ... "
Reference:
1 . Ait Ouares K, Filipis L, Tzilivaki A, Poirazi P, Canepari M (2019) Two Distinct Sets of Ca2+ and K+ Channels Are Activated at Different Membrane Potentials by the Climbing Fiber Synaptic Potential in Purkinje Neuron Dendrites. J Neurosci 39:1969-1981 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Dendrite;
Brain Region(s)/Organism: Cerebellum;
Cell Type(s): Cerebellum Purkinje GABA cell;
Channel(s): I Calcium; I K,Ca; I Potassium;
Gap Junctions:
Receptor(s):
Gene(s):
Transmitter(s):
Simulation Environment: NEURON;
Model Concept(s): Simplified Models; Calcium dynamics; Active Dendrites;
Implementer(s): Filipis, Luiza [luizafilipu at gmail.com];
Search NeuronDB for information about:  Cerebellum Purkinje GABA cell; I K,Ca; I Calcium; I Potassium;
{
xpanel("RunControl", 0)
v_init = -87
xvalue("Init","v_init", 1,"stdinit()", 1, 1 )
xbutton("Init & Run","run()")
xbutton("Stop","stoprun=1")
runStopAt = 20
xvalue("Continue til","runStopAt", 1,"{continuerun(runStopAt) stoprun=1}", 1, 1 )
t = 20
xvalue("t","t", 2 )
tstop = 20
xvalue("Tstop","tstop", 1,"tstop_changed()", 0, 1 )
dt = 0.002
xvalue("dt","dt", 1,"setdt()", 0, 1 )
steps_per_ms = 5
xvalue("Points plotted/ms","steps_per_ms", 1,"setdt()", 0, 1 )
realtime = 0.19
xvalue("Real Time","realtime", 0,"", 0, 1 )
xlabel(" ")
xlabel("CF Current")
g_climbing = 0.002
xvalue("Ampl","g_climbing", 1,"", 0, 0 )
icin_climbing = 0.044
xvalue("Initial","icin_climbing", 1,"", 0, 0 )
del_climbing = 2.2
xvalue("Delay","del_climbing", 1,"", 0, 0 )
e_climbing = -200
xvalue("A","e_climbing", 1,"", 0, 0 )
tauC_climbing = 3
xvalue("dur","tauC_climbing", 1,"", 0, 0 )
tauO_climbing = 1.4
xvalue("rise","tauO_climbing", 1,"", 0, 0 )
delf_climbing = 2
xvalue("Delayf","delf_climbing", 1,"", 0, 0 )
ef_climbing = -150
xvalue("Af","ef_climbing", 1,"", 0, 0 )
tauCf_climbing = 6
xvalue("durf","tauCf_climbing", 1,"", 0, 0 )
tauOf_climbing = 5
xvalue("risef","tauOf_climbing", 1,"", 0, 0 )
fastfact_climbing = 0
xvalue("fact","fastfact_climbing", 1,"", 0, 0 )
xlabel(" ")
xlabel("Buffer")
Buffnull2_cdp5 = 0
xvalue("Total","Buffnull2_cdp5", 1,"", 0, 0 )
rf3_cdp5 = 0.14352
xvalue("k1","rf3_cdp5", 1,"", 0, 0 )
rf4_cdp5 = 0.0013626
xvalue("k2","rf4_cdp5", 1,"", 0, 0 )
xpanel(512,54)
}
{
xpanel("Buffers", 0)
BTCnull_cdp5 = 1
xvalue("Dye","BTCnull_cdp5", 1,"", 0, 0 )
b1_cdp5 = 570
xvalue("Dye-Kon","b1_cdp5", 1,"", 0, 0 )
b2_cdp5 = 5.7
xvalue("Dye-Koff","b2_cdp5", 1,"", 0, 0 )
DMNPEnull_cdp5 = 0.75
xvalue("Imobile","DMNPEnull_cdp5", 1,"", 0, 0 )
c1_cdp5 = 570
xvalue("Imob-Kon","c1_cdp5", 1,"", 0, 0 )
c2_cdp5 = 4
xvalue("Imob-Koff","c2_cdp5", 1,"", 0, 0 )
CBnull_cdp5 = 0.6
xvalue("Calbindin","CBnull_cdp5", 1,"", 0, 0 )
nf1_cdp5 = 217.5
xvalue("CBf-Kon","nf1_cdp5", 1,"", 0, 0 )
nf2_cdp5 = 0.0358
xvalue("CBf-Koff","nf2_cdp5", 1,"", 0, 0 )
ns1_cdp5 = 27.5
xvalue("CBs-Kon","ns1_cdp5", 1,"", 0, 0 )
ns2_cdp5 = 0.0026
xvalue("CBs-Koff","ns2_cdp5", 1,"", 0, 0 )
PVnull_cdp5 = 0.075
xvalue("Parvalbumin","PVnull_cdp5", 1,"", 0, 0 )
m1_cdp5 = 535
xvalue("PVca-Kon","m1_cdp5", 1,"", 0, 0 )
m2_cdp5 = 0.00095
xvalue("PVca-Koff","m2_cdp5", 1,"", 0, 0 )
p1_cdp5 = 4
xvalue("PVmg-Kon","p1_cdp5", 1,"", 0, 0 )
p2_cdp5 = 0.025
xvalue("PVmg-Koff","p2_cdp5", 1,"", 0, 0 )
xpanel(48,870)
}
{
xpanel("(Parameters)", 0)
gbar_leak = 0.002
xvalue("leak","gbar_leak", 1,"", 0, 0 )
pcabar_CaT3_1 = 0.00015
xvalue("Tm","pcabar_CaT3_1", 1,"", 0, 0 )
t1_CaT3_1 = 0.3
xvalue("t1","t1_CaT3_1", 1,"", 0, 0 )
t2_CaT3_1 = 53
xvalue("t2","t2_CaT3_1", 1,"", 0, 0 )
xlabel("A")
gkbar_KA1 = 0.07
xvalue("A","gkbar_KA1", 1,"", 0, 0 )
vm1_KA1 = 50
xvalue("left","vm1_KA1", 1,"", 0, 0 )
vm2_KA1 = 30
xvalue("up","vm2_KA1", 1,"", 0, 0 )
t1_KA1 = 0.2
xvalue("t1","t1_KA1", 1,"", 0, 0 )
t2_KA1 = 50
xvalue("t2","t2_KA1", 1,"", 0, 0 )
pcabar_newCaP1 = 0.00075
xvalue("PQm","pcabar_newCaP1", 1,"", 0, 0 )
pp_newCaP1 = 0.6
xvalue("t","pp_newCaP1", 1,"", 0, 0 )
t1_newCaP1 = 0.1
xvalue("c1","t1_newCaP1", 1,"", 0, 0 )
t2_newCaP1 = 50
xvalue("c2","t2_newCaP1", 1,"", 0, 0 )
xlabel("Kdr")
gkbar_Kdr1 = 0.032
xvalue("Kdr","gkbar_Kdr1", 1,"", 0, 0 )
pp_Kdr1 = 18
xvalue("t1","pp_Kdr1", 1,"", 0, 0 )
pdr_Kdr1 = 1
xvalue("t2","pdr_Kdr1", 1,"", 0, 0 )
vsh1_Kdr1 = 0
xvalue("vact","vsh1_Kdr1", 1,"", 0, 0 )
vsh2_Kdr1 = 0
xvalue("vinact","vsh2_Kdr1", 1,"", 0, 0 )
hdr_Kdr1 = 1
xvalue("tinact","hdr_Kdr1", 1,"", 0, 0 )
xlabel("K-Ca")
gkbar_KC3 = 2.500
xvalue("BK","gkbar_KC3", 1,"", 0, 0 )
gkbar_SK2 = 0.03
xvalue("SK","gkbar_SK2", 1,"", 0, 0 )
xpanel(156,126)
}

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