DRG neuron models investigate how ion channel levels regulate firing properties (Zheng et al 2019)

 Download zip file   Auto-launch 
Help downloading and running models
Accession:256632
We present computational models for an Abeta-LTMR (low-threshold mechanoreceptor) and a C-LTMR expressing four Na channels and four K channels to investigate how the expression level of Kv1 and Kv4 regulate number of spikes (repetitive firing) and onset latency to action potentials in Abeta-LTMRs and C-LTMRs, respectively.
Reference:
1 . Zheng Y, Liu P, Bai L, Trimmer JS, Bean BP, Ginty DD (2019) Deep Sequencing of Somatosensory Neurons Reveals Molecular Determinants of Intrinsic Physiological Properties Neuron
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell;
Brain Region(s)/Organism: Mouse;
Cell Type(s): Dorsal Root Ganglion (DRG) cell;
Channel(s): I Sodium; I Potassium; I K; I A;
Gap Junctions:
Receptor(s):
Gene(s): Nav1.1 SCN1A; Nav1.6 SCN8A; Nav1.7 SCN9A; Nav1.8 SCN10A; Kv1.1 KCNA1; Kv1.2 KCNA2; Kv2.1 KCNB1; Kv3.1 KCNC1; Kv3.3 KCNC3; Kv3.4 KCNC4; Kv4.3 KCND3;
Transmitter(s):
Simulation Environment: NEURON; R;
Model Concept(s): Action Potential Initiation; Action Potentials; Activity Patterns; Delay; Ion Channel Kinetics; Membrane Properties;
Implementer(s): Zheng, Yang [zylittlep at gmail.com]; Bean, Bruce [bruce_bean at hms.harvard.edu];
Search NeuronDB for information about:  I A; I K; I Sodium; I Potassium;
{load_file("nrngui.hoc")}
objectvar save_window_, rvp_
objectvar scene_vector_[6]
objectvar ocbox_, ocbox_list_, scene_, scene_list_
{ocbox_list_ = new List()  scene_list_ = new List()}
{pwman_place(0,0,0)}

//Begin PointProcessGroupManager
{
load_file("pointgrp.hoc")
}
{
ocbox_ = new PointProcessGroupManager(0)
}
{object_push(ocbox_)}
{
ms = new MechanismStandard("IClamp")
ms.set("del", 100, 0)
ms.set("dur", 500, 0)
ms.set("amp", 0, 0)
mnews("IClamp", ms)
select(0)
execute("soma ocbox_.move(0.5)")
ms = new MechanismStandard("APCount")
ms.set("n", 1, 0)
ms.set("thresh", -12, 0)
ms.set("time", 103.15, 0)
mnews("APCount", ms)
select(1)
execute("soma ocbox_.move(0.5)")
}
{object_pop() doNotify()}
{
ocbox_ = ocbox_.v1
ocbox_.map("PointProcessGroupManager", 9, 542, 553.92, 194.88)
}
objref ocbox_
//End PointProcessGroupManager

{
save_window_ = new Graph(0)
save_window_.size(0,700,-80,40)
scene_vector_[3] = save_window_
{save_window_.view(0, -80, 700, 120, 684, 0, 556.8, 180.16)}
graphList[0].append(save_window_)
save_window_.save_name("graphList[0].")
save_window_.addexpr("v(.5)", 1, 1, 0.8, 0.9, 2)
}

//Begin Grapher
{
load_file("grapher.hoc")}
{
ocbox_=new Grapher(1)

ocbox_.info("IClamp[0].amp","IClamp[0].amp", "run()", 0, 10, -10, 10, 75, 0, 1.5)
{
save_window_=ocbox_.g
save_window_.size(0,10,-10,10)
scene_vector_[4] = save_window_
ocbox_.g = save_window_
save_window_.save_name("ocbox_.g")
save_window_.xexpr("IClamp[0].amp", 0)
save_window_.addvar("APCount[0].n", 1, 1, 0.735268, 0.839562, 2)
}
ocbox_ = ocbox_.vbox
ocbox_.map("Grapher", 296, 0, 367.68, 488.64)
}
objref ocbox_
//End Grapher

{
xpanel("RunControl", 0)
v_init = -80
xvalue("Init","v_init", 1,"stdinit()", 1, 1 )
xbutton("Init & Run","run()")
xbutton("Stop","stoprun=1")
runStopAt = 5
xvalue("Continue til","runStopAt", 1,"{continuerun(runStopAt) stoprun=1}", 1, 1 )
runStopIn = 1
xvalue("Continue for","runStopIn", 1,"{continuerun(t + runStopIn) stoprun=1}", 1, 1 )
xbutton("Single Step","steprun()")
t = 700
xvalue("t","t", 2 )
tstop = 700
xvalue("Tstop","tstop", 1,"tstop_changed()", 0, 1 )
dt = 0.025
xvalue("dt","dt", 1,"setdt()", 0, 1 )
steps_per_ms = 40
xvalue("Points plotted/ms","steps_per_ms", 1,"setdt()", 0, 1 )
screen_update_invl = 0.05
xvalue("Scrn update invl","screen_update_invl", 1,"", 0, 1 )
realtime = 0.1
xvalue("Real Time","realtime", 0,"", 0, 1 )
xpanel(3,113)
}
{
save_window_ = new Graph(0)
save_window_.size(0,700,-1,1)
scene_vector_[5] = save_window_
{save_window_.view(0, -1, 700, 2, 684, 243, 549.12, 400)}
graphList[1].append(save_window_)
save_window_.save_name("graphList[1].")
save_window_.addvar("soma.ik_kv1( 0.5 )", 3, 1, 0.714333, 0.9, 2)
save_window_.addvar("soma.ik_kv2( 0.5 )", 4, 1, 0.720923, 0.9, 2)
save_window_.addvar("soma.ik_kv3( 0.5 )", 2, 1, 0.72257, 0.9, 2)
save_window_.addvar("soma.ik_kv4( 0.5 )", 7, 1, 0.724217, 0.9, 2)
save_window_.addvar("soma.ina_nav1p1( 0.5 )", 3, 1, 0.697858, 0.549961, 2)
save_window_.addvar("soma.ina_nav1p6( 0.5 )", 4, 1, 0.696211, 0.542913, 2)
save_window_.addvar("soma.ina_nav1p7( 0.5 )", 1, 1, 0.697858, 0.540564, 2)
save_window_.addvar("soma.ina_nav1p8( 0.5 )", 9, 1, 0.699506, 0.533516, 2)
}
objectvar scene_vector_[1]
{doNotify()}

Loading data, please wait...