CA1 pyramidal neuron: dendritic spike initiation (Gasparini et al 2004)

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Accession:44050
NEURON mod files from the paper: Sonia Gasparini, Michele Migliore, and Jeffrey C. Magee On the initiation and propagation of dendritic spikes in CA1 pyramidal neurons, J. Neurosci., J. Neurosci. 24:11046-11056 (2004).
Reference:
1 . Gasparini S, Migliore M, Magee JC (2004) On the initiation and propagation of dendritic spikes in CA1 pyramidal neurons. J Neurosci 24:11046-56 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Dendrite;
Brain Region(s)/Organism:
Cell Type(s): Hippocampus CA1 pyramidal GLU cell;
Channel(s): I Na,t; I A; I K; I h;
Gap Junctions:
Receptor(s): AMPA; NMDA;
Gene(s):
Transmitter(s): Glutamate;
Simulation Environment: NEURON;
Model Concept(s): Action Potential Initiation; Dendritic Action Potentials; Coincidence Detection; Active Dendrites; Detailed Neuronal Models; Action Potentials;
Implementer(s): Migliore, Michele [Michele.Migliore at Yale.edu];
Search NeuronDB for information about:  Hippocampus CA1 pyramidal GLU cell; AMPA; NMDA; I Na,t; I A; I K; I h; Glutamate;
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gaspiriniEtAl2004
readme.txt *
exp2i.mod *
h.mod *
kadist.mod *
kaprox.mod *
kdrca1.mod *
na3s.mod *
naxn.mod *
netstims.mod *
nmdanet.mod *
forfig5A.hoc *
mosinit.hoc *
n160.nrn *
                            
load_file("nrngui.hoc")
cvode_active(1)

dist=14
rel=0.75

nsyn=5
weight=0.02

Rm = 28000
RmDend = Rm/2
RmSoma = Rm
RmAx = Rm

Cm    = 1
CmSoma= Cm
CmAx  = Cm
CmDend = Cm*2

RaAll= 150
RaSoma=150  
RaAx = 50

Vrest = -65
dt = 0.01
gna =  .03
AXONM = 2
gkdr = 0.005
celsius = 35.0  
KMULT =  0.022
KMULTP = 0.022
ghd=0.00005
nash=10

xopen("n160.nrn")             // geometry file

access soma[5]
distance()

tstop=20

objref g, b,c, stim, p, s[nsyn], rsyn[nsyn], nc[nsyn], rc, rd, rs
objref rsynmda[nsyn], ncnmda[nsyn]

b = new VBox()
b.intercept(1)
g = new Graph()
g.size(0,tstop,-70,-10)
g.family(1)
g.addvar("apical[dist].v(rel)",2,2,2*tstop,0,2)
g.xaxis(1)
xpanel(" ",1)
xbutton("current ", "runc()")
xbutton("AMPA", "runa()")
xbutton("AMPA+NMDA", "runm()")
xpanel()
b.intercept(0)
b.map("gasparini et al.", 100,100,250,500)

apical[dist] { 
print distance(rel)
stim= new IClamp(rel)
stim.amp=0
stim.dur=150
stim.del=1
	for i=0, nsyn-1 {
		s[i] = new NetStims(rel)
		s[i].interval=0
		s[i].number = 1
		s[i].start=1.e9
		s[i].noise=0
		rsynmda[i] = new nmdanet(rel)
		rsyn[i] = new Exp2Syn(rel)
		rsyn[i].e=0
		rsyn[i].tau1 = .5
		rsyn[i].tau2 = 1
		nc[i] = new NetCon(s[i],rsyn[i],0,0,4.2e-3)
                ncnmda[i] = new NetCon(s[i],rsynmda[i],0,0,1*0.0035)
	}
}

axon[1] {
                insert nax gbar_nax=gna * AXONM	sh_nax=3
                insert kdr gkdrbar_kdr=gkdr
                insert pas e_pas=Vrest g_pas = 1/RmAx Ra=RaAx cm=CmAx
                insert kap gkabar_kap = KMULTP*0.2
}

axon[0] {   
                insert nax  gbar_nax=gna * AXONM sh_nax=3
                insert kdr  gkdrbar_kdr=gkdr 
                insert pas e_pas=Vrest g_pas = 1/RmAx Ra=RaAx cm=CmAx
                insert kap gkabar_kap = KMULTP*0.2
}

for i=0,NumSoma-1 soma[i] {   
		insert hd ghdbar_hd=ghd	vhalfl_hd=-73
                insert nas  gbar_nas=gna sh_nas=nash ar_nas=1
                insert kdr gkdrbar_kdr=gkdr
                insert kap gkabar_kap = KMULTP
                insert pas e_pas=Vrest g_pas = 1/RmSoma Ra=RaSoma cm=CmSoma
}


for i=0,NumBasal-1 basal[i] {
                insert nas gbar_nas=gna sh_nas=nash ar_nas=1
                insert kdr gkdrbar_kdr=gkdr 
                insert kap gkabar_kap=KMULTP
                insert pas e_pas=Vrest g_pas = 1/RmDend Ra=RaAll cm=CmDend
}
                
for i=0,NumApical-1 apical[i] {
              insert pas e_pas=Vrest g_pas = 1/RmDend Ra=RaAll  cm=CmDend
		insert hd ghdbar_hd=ghd
                insert nas sh_nas=nash ar_nas=1	gbar_nas=gna
                insert kdr gkdrbar_kdr=gkdr
		insert kap
		insert kad
		gkabar_kap=0
		gkabar_kad=0

              for (x){ xdist = distance(x)
                if (xdist>500) {xdist=500}
                ghdbar_hd(x) = ghd*(1+3*xdist/100)
                if (xdist > 100){
			if (xdist>300) {ndist=300} else {ndist=xdist}
			sh_nas=nash-8*(ndist-100)/200
			vhalfl_hd(x)=-73-8*(ndist-100)/200
                        gkabar_kad(x) = KMULT*(1+xdist/100)
			gkabar_kap(x)=0
                } else {
			vhalfl_hd(x)=-73
                        gkabar_kap(x) = KMULTP*(1+xdist/100)
			gkabar_kad(x)=0
                	}
              				}
				}


proc init() {
	t=0
        forall {
	v=Vrest
        if (ismembrane("nas") || ismembrane("nax") ) {ena=55}
        if (ismembrane("kdr") || ismembrane("kap") || ismembrane("kad")) {ek=-90}
        if (ismembrane("hd") ) {ehd_hd=-30 }
}
	finitialize(v)
        fcurrent()

        forall {
	for (x) {
		e_pas(x)=v(x)
		if (!ismembrane("hd")){e_pas(x)=e_pas(x)+(ina(x)+ik(x))/g_pas(x)}
		if (ismembrane("hd")) {e_pas(x)=e_pas(x)+(ina(x)+ik(x)+i_hd(x))/g_pas(x)}
}
		}

cvode.re_init()
cvode.event(tstop)
g.begin()
g.plot(t)

}

proc advance() {
	fadvance()
	g.plot(t)
	g.flush()
	doNotify()
}

proc runm() {
apical[dist] {
for i=0, nsyn-1 {
	rsyn[i] = new Exp2Syn(rel)
	rsyn[i].e=0
	rsyn[i].tau1 = .5
	rsyn[i].tau2 = 1
	nc[i] = new NetCon(s[i],rsyn[i],0,0,4.2e-3)
	ncnmda[i].weight = 1*0.015
	}
}
g.erase()
g.color(1)
g.label(0.5,0.8,"dt = .1")
	for i=0, nsyn-1 {s[i].start=1+i*.1}
run()

g.color(2)
g.label(0.5,0.75,"dt = 0.2")
	for i=0, nsyn-1 {s[i].start=1+i*0.2}
run()

g.color(3)
g.label(0.5,0.7,"dt = 0.5")
	for i=0, nsyn-1 {s[i].start=1+i*0.5}
run()

g.color(4)
g.label(0.5,0.65,"dt = 1")
	for i=0, nsyn-1 {s[i].start=1+i*1}
run()
}

proc runa() {
apical[dist] {
for i=0, nsyn-1 {
	rsyn[i] = new Exp2Syn(rel)
	rsyn[i].e=0
	rsyn[i].tau1 = .5
	rsyn[i].tau2 = 1
	nc[i] = new NetCon(s[i],rsyn[i],0,0,5.2e-3)
	ncnmda[i].weight = 0*0.015
	}
}
g.erase()
g.color(1)
g.label(0.5,0.8,"dt = .1")
	for i=0, nsyn-1 {s[i].start=1+i*.1}
run()

g.color(2)
g.label(0.5,0.75,"dt = 0.2")
	for i=0, nsyn-1 {s[i].start=1+i*0.2}
run()

g.color(3)
g.label(0.5,0.7,"dt = 0.5")
	for i=0, nsyn-1 {s[i].start=1+i*0.5}
run()

g.color(4)
g.label(0.5,0.65,"dt = 1")
	for i=0, nsyn-1 {s[i].start=1+i*1}
run()
}

proc runc() {
apical[dist] {
for i=0, nsyn-1 {
	rsyn[i] = new Exp2i(rel)
	rsyn[i].tau1 = .5
	rsyn[i].tau2 = 1
	nc[i] = new NetCon(s[i],rsyn[i],0,0,.24)
	ncnmda[i].weight = 0*0.015
	}
}
g.erase()
g.color(1)
g.label(0.5,0.8,"dt = .1")
	for i=0, nsyn-1 {s[i].start=1+i*.1}
run()

g.color(2)
g.label(0.5,0.75,"dt = 0.2")
	for i=0, nsyn-1 {s[i].start=1+i*0.2}
run()

g.color(3)
g.label(0.5,0.7,"dt = 0.5")
	for i=0, nsyn-1 {s[i].start=1+i*0.5}
run()

g.color(4)
g.label(0.5,0.65,"dt = 1")
	for i=0, nsyn-1 {s[i].start=1+i*1}
run()
}

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