"Cerebellar stellate cells are inhibitory molecular interneurons that regulate the firing properties of Purkinje cells, the sole output of cerebellar cortex. Recent evidence suggests that
these cells exhibit temporal increase in excitability during whole-cell patch-clamp configuration in a phenomenon termed runup. They also exhibit a non-monotonic first-spike
latency profile as a function of the holding potential in response to a fixed step-current.
In this study, we use modeling approaches to unravel the dynamics of runup and categorize the firing behavior of cerebellar stellate cells as either type I or type II oscillators. We
then extend this analysis to investigate how the non-monotonic latency profile manifests
itself during runup. We employ a previously developed, but revised, Hodgkin–Huxley type
model to show that stellate cells are indeed type I oscillators possessing a saddle node on
an invariant cycle (SNIC) bifurcation. The SNIC in the model acts as a “threshold” for tonic
firing and produces a slow region in the phase space called the ghost of the SNIC. The
model reveals that (i) the SNIC gets left-shifted during runup with respect to I app = I test
in the current-step protocol, and (ii) both the distance from the stable limit cycle along
with the slow region produce the non-monotonic latency profile as a function of holding
potential. Using the model, we elucidate how latency can be made arbitrarily large for a
specific range of holding potentials close to the SNIC during pre-runup (post-runup). We
also demonstrate that the model can produce transient single spikes in response to step-
currents entirely below I SNIC , and that a pair of dynamic inhibitory and excitatory post-
synaptic inputs can robustly evoke action potentials, provided that the magnitude of the
inhibition is either low or high but not intermediate. Our results show that the topology
of the SNIC is the key to explaining such behaviors."
Reference:
1 .
Mitry J, Alexander RP, Farjami S, Bowie D, Khadra A (2020) Modeling excitability in cerebellar stellate cells: Temporal changes in threshold, latency and frequency of firing Communications in Nonlinear Science and Numerical Simulation 82:105014
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