Cl- homeostasis in immature hippocampal CA3 neurons (Kolbaev et al 2020)

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Accession:266811
Model used for the revision of the manuscript. Insertion of a passive Cl- flux and an active Cl-accumulation. Parameters adapted to match the properties of [Cl-]i determined in immature rat CA3 neurons in-vitro.
Reference:
1 . Kolbaev SN, Mohapatra N, Chen R, Lombardi A, Staiger JF, Luhmann HJ, Jedlicka P, Kilb W (2020) NKCC-1 mediated Cl- uptake in immature CA3 pyramidal neurons is sufficient to compensate phasic GABAergic inputs. Sci Rep 10:18399 [PubMed]
Model Information (Click on a link to find other models with that property)
Model Type: Neuron or other electrically excitable cell; Synapse;
Brain Region(s)/Organism: Hippocampus;
Cell Type(s):
Channel(s): NKCC1;
Gap Junctions:
Receptor(s): GabaA;
Gene(s):
Transmitter(s): Gaba;
Simulation Environment: NEURON;
Model Concept(s): Synaptic Plasticity; Homeostasis;
Implementer(s): Jedlicka, Peter [jedlicka at em.uni-frankfurt.de]; Kilb, Werner [wkilb at uni-mainz.de];
Search NeuronDB for information about:  GabaA; NKCC1; Gaba;
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Models_Kolbaev et al Scientific Reports Revision
Readme.txt
asin.mod *
cldif_CA3_NKCC1_HCO3.mod *
gabaA_Cl_HCO3.mod *
tonic.mod *
vecevent.mod *
Add_tonic_Cl-current.hoc
anyl.m
Block-Tonic-Cl-current.ses
Cell_1_SciRep_ShrinkCorr.hoc
Determine_cl-Flux_w-o_NKCC1_rig.ses
Determine_R_input_rig.ses
Determine_tau_NKCC1_rig.ses
Display_Phasic-Cl-current.ses
Display_Phasic-Cl-current_for_Charge_Transfer.ses
Display_Phasic-Cl-current_forFreq.ses
Phasic_GABA_activity_Div_Freq.hoc
Phasic_GABA_activity_Div_gGABA.hoc
Phasic_GABA_activity_only_soma_Backregul.hoc
Phasic_GABA_activity_only_soma_Div_Freq.hoc
Phasic_GABA_activity_only_soma_Div_gGABA.hoc
Phasic_GABA_activity_only_soma_for_Charge_Transfer.hoc
Phasic_GABA_activity_only_soma_PlaceSynapsesForFigure.hoc
start_Add_Tonic_Cl-currents.hoc
start_Block_Tonic_Cl-currents.hoc
start_Phasic_Cl-currents.hoc
start_Phasic_Cl-currents_for _Change-transfer.hoc
start_Phasic_GABA_activity_Div_Freq.hoc
start_Phasic_GABA_activity_only_soma_Backregul.hoc
start_Phasic_GABA_activity_only_soma_Div_Freq.hoc
start_Phasic_GABA_activity_only_soma_Div_gGABA.hoc
Switch_off_tonic_Cl-current.hoc
                            
clear all 

offcl = importdata('c0.dat');
loc0 = importdata('loc1.dat');
offcl1 = importdata('c1.dat');
offcl2 = importdata('c3.dat');
offcl3 = importdata('c4.dat');

%t = 0:0.025:1000;
dt = 0.25
t100 = (100/dt)+1;
t500 = (500/dt)+1;
t800 = (800/dt)+1;
t1000 = (1000/dt)+1;

plot(loc0,offcl(:,t100),':','Color','red')
hold on 
plot(loc0,offcl(:,t500),':','Color','black')
hold on 
plot(loc0,offcl(:,t800),':','Color','blue')
hold on 
plot(loc0,offcl(:,t1000),':','Color','green')
box off 
hold on 

plot(loc0,offcl1(:,t100),'red')
hold on 
plot(loc0,offcl1(:,t500),'black')
hold on 
plot(loc0,offcl1(:,t800),'blue')
hold on 
plot(loc0,offcl1(:,t1000),'green')
box off 

plot(loc0,offcl2(:,t100),'--','Color','red')
hold on 
plot(loc0,offcl2(:,t500),'--','Color','black')
hold on 
plot(loc0,offcl2(:,t800),'--','Color','blue')
hold on 
plot(loc0,offcl2(:,t1000),'--','Color','green')
box off 
hold on 

plot(loc0,offcl3(:,t100),'-o','Color','red')
hold on 
plot(loc0,offcl3(:,t500),'-o','Color','black')
hold on 
plot(loc0,offcl3(:,t800),'-o','Color','blue')
hold on 
plot(loc0,offcl3(:,t1000),'-o','Color','green')
box off 

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