func fieldrec() { local I, Phi, PhiAll, r, x, y, z, x1, x2, y1, y2, z1, z2, dS, h, l, r2 sigma = 25*0.3 // uS/um PhiAll = 0 forall { if (ismembrane("extracellular")) { I = 0 for (x,0) { I += i_membrane(x)*area(x) } if( n3d() != 2 ) print "n3d() not 2, line approximation is invalid, section: ", secname() // coordinates of the line ends: x1 = x3d(0) x2 = x3d(1) y1 = y3d(0) y2 = y3d(1) z1 = z3d(0) z2 = z3d(1) dS = arc3d(1) // distances: h = (1/dS) * ( (Ex - x2)*(x2 - x1) + (Ey - y2)*(y2 - y1) + (Ez - z2)*(z2 - z1) ) r2 = (Ex - x2)^2 + (Ey - y2)^2 + (Ez - z2)^2 - h^2 l = h + dS if (h<0 && l<0) { Phi = (I/(4*PI*sigma*dS)) * log( (sqrt(h^2 + r2) - h) / (sqrt(l^2 + r2) - l) ) } if (h<0 && l>0) { Phi = (I/(4*PI*sigma*dS)) * log( (sqrt(h^2 + r2) - h) * ( l + sqrt(l^2 + r2) ) / r2) } if (h>0 && l>0) { Phi = (I/(4*PI*sigma*dS)) * log( (l + sqrt(l^2 + r2) ) / ( sqrt(h^2 + r2) + h )) } ifsec "soma" { //treat it as a sphere x = (x2 + x1)/2 y = (y2 + y1)/2 z = (z2 + z1)/2 r = sqrt( (Ex-x)^2 + (Ey-y)^2 + (Ez-z)^2 ) Phi = I/(4*PI*sigma*r) } PhiAll += Phi } } return PhiAll } func totalI() { local I I = 0 forall { if (ismembrane("extracellular")) { for (x,0) { I += i_membrane(x)*area(x) } } } return I } vrec = 0 //irec = 0 proc init() { finitialize(v_init) fcurrent() vrec = fieldrec() // irec = totalI() } proc advance() { fadvance() vrec = fieldrec() // irec = totalI() } objref Vrec Vrec = new Vector() Vrec.record(&vrec,0.2) // Dt=0.2 ms, so sampling f=5 KHz proc writeLFP() { localobj fout // arg $1 must be file name, e.g. "myfile.ext" fout=new File() fout.wopen($s1) Vrec.printf(fout, "%4.2f\t") fout.close() }