TITLE N-type calcium channel for nucleus accumbens neuron
: comments at end of file
UNITS {
(mV) = (millivolt)
(mA) = (milliamp)
(S) = (siemens)
(molar) = (1/liter)
(mM) = (millimolar)
FARADAY = (faraday) (coulomb)
R = (k-mole) (joule/degC)
}
NEURON {
SUFFIX can
USEION ca READ cai, cao WRITE ica
RANGE pbar, ica
POINTER mu
}
PARAMETER {
pbar = 1.0e-5 (cm/s) : vh = -100 mV, 120 ms pulse to 0 mV
mvhalf = -8.7 (mV) : Churchill 1998, fig 5
mslope = -7.4 (mV) : Churchill 1998, fig 5
mshift = 0 (mV)
hvhalf = -74.8 (mV) : McNaughton 1997, Table 1
hslope = 6.5 (mV) : McNaughton 1997, Table 1
hshift = 0 (mV)
htau = 70.0 (ms) : McNaughton 1997, Table 2 (tau1 in Ca)
vm = -17.19 (mV) : fit from Kasai 1992, fig 15
k = 15.22 (mV) : fit from Kasai 1992, fig 15
kpr = 23.82 (mV) : fit from Kasai 1992, fig 15
c = 0.03856 (/ms-mV) : fit from Kasai 1992, fig 15
cpr = 0.3842 (/ms) : fit from Kasai 1992, fig 15
a = 0.21 : percent inactivation
qfact = 3 : both m & h recorded at 22 C
}
ASSIGNED {
v (mV)
ica (mA/cm2)
celsius (degC)
cai (mM)
cao (mM)
minf
mtau (ms)
hinf
mu (1)
}
STATE {
m h
}
BREAKPOINT {
SOLVE states METHOD cnexp
ica = ghk(v,cai,cao) * pbar * m * m * (a*h + (1-a))*(1-(mu-1)*0.8) : Kasai 92, Brown 93
}
INITIAL {
settables(v)
m = minf
h = hinf
}
DERIVATIVE states {
settables(v)
m' = (minf - m) / (mtau/qfact)
h' = (hinf - h) / (htau/qfact)
}
PROCEDURE settables( v (mV) ) {
LOCAL malpha, mbeta
TABLE minf, hinf, mtau DEPEND mshift, hshift
FROM -100 TO 100 WITH 201
minf = 1 / ( 1 + exp( (v-mvhalf-mshift) / mslope) )
hinf = 1 / ( 1 + exp( (v-hvhalf-hshift) / hslope) )
malpha = c * (v-vm) / ( exp((v-vm)/k) - 1 )
mbeta = cpr * exp(v/kpr) : Kasai 1992, fig 15
mtau = 1 / (malpha + mbeta)
}
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
: ghk() borrowed from cachan.mod share file in Neuron
FUNCTION ghk(v(mV), ci(mM), co(mM)) (.001 coul/cm3) {
LOCAL z, eci, eco
z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))
eco = co*efun(z)
eci = ci*efun(-z)
:high cao charge moves inward
:negative potential charge moves inward
ghk = (.001)*2*FARADAY*(eci - eco)
}
FUNCTION efun(z) {
if (fabs(z) < 1e-4) {
efun = 1 - z/2
}else{
efun = z/(exp(z) - 1)
}
}
COMMENT
Brown AM, Schwindt PC, Crill WE (1993) Voltage dependence and activation
kinetics of pharmacologically defined components of the high-threshold
calcium current in rat neocortical neurons. J Neurophysiol 70:1530-1543.
Churchill D, Macvicar BA (1998) Biophysical and pharmacological
characterization of voltage-dependent Ca2+ channels in neurons isolated
from rat nucleus accumbens. J Neurophysiol 79:635-647.
Kasai H, Neher E (1992) Dihydropyridine-sensitive and
omega-conotoxin-sensitive calcium channels in a mammalian
neuroblastoma-glioma cell line. J Physiol 448:161-188.
Koch, C., and Segev, I., eds. (1998). Methods in Neuronal Modeling: From
Ions to Networks, 2 edn (Cambridge, MA, MIT Press).
Hille, B. (1992). Ionic Channels of Excitable Membranes, 2 edn
(Sunderland, MA, Sinauer Associates Inc.).
McNaughton NCL, Randall AD (1997) Electrophysiological properties of the
human N-type calcium Ca2+ channel: I. Channel Gating in Ca2+, Ba2+, and
Sr2+ containing solutions. Neuropharm 36:895-915.
This is the w-conotoxin GV1A sensitive current in fig 5 from Churchill.
The standard HH model uses a linear approximation to the driving force
for an ion: (Vm - ez). This is ok for na and k, but not ca - calcium
rectifies at high potentials because
1. internal and external concentrations of ca are so different,
making outward current flow much more difficult than inward
2. calcium is divalent so rectification is more sudden than for na
and k. (Hille 1992, pg 107)
Accordingly, we need to replace the HH formulation with the GHK model,
which accounts for this phenomenon. The GHK equation is eq 6.6 in Koch
1998, pg 217 - it expresses Ica in terms of Ca channel permeability
(Perm,ca) times a mess. The mess can be circumvented using the ghk
function below, which is included in the Neuron share files. Perm,ca
can be expressed in an HH-like fashion as
Perm,ca = pcabar * mca * mca (or however many m's and h's)
where pcabar has dimensions of permeability but can be thought of as max
conductance (Koch says it should be about 10^7 times smaller than the HH
gbar - dont know) and mca is analagous to m (check out Koch 1998 pg 144)
Calcium current can then be modeled as
ica = pcabar * mca * mca * ghk()
Jason Moyer 2004 - jtmoyer@seas.upenn.edu
ENDCOMMENT